Influence of food processing environments on structure initiation of static biofilm of Listeria monocytogenes

Pilchová, Tereza; Hernould, Michel; Prévost, Hervé; Demnerová, Kateřina; Pazlarová, J.; Tresse, Odile

doi:10.1016/j.foodcont.2013.07.021

Cited by 54 publications

(26 citation statements)

References 55 publications

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“…Borucki et al 4 reported L. monocytogenes produced a dense 3-D biofilm structure, while Pilchová et al 17 demonstrated also a honeycomb-like, as the present study. The uneven colonization of abiotic surfaces is not uncommon for L. monocytogenes in biofilms,6, 18, 19 although there is also a literature report on a thin biofilm structure covering most of the abiotic surface 3 .…”

Section: Discussionsupporting

confidence: 76%

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces

Reis‐Teixeira

Alves

Martinis

2017

Brazilian Journal of Microbiology

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The pathogenic bacterium Listeria monocytogenes can persist in food processing plants for many years, even when appropriate hygienic measures are in place, with potential for contaminating ready-to-eat products and, its ability to form biofilms on abiotic surfaces certainly contributes for the environmental persistence. In this research, L. monocytogenes was grown in biofilms up 8 days attached to stainless steel and glass surfaces, contributing for advancing the knowledge on architecture of mature biofilms, since many literature studies carried out on this topic considered only early stages of cell adhesion. In this study, biofilm populations of two strains of L. monocytogenes (serotypes 1/2a and 4b) on stainless steel coupons and glass were examined using regular fluorescence microscopy, confocal laser scanning microscopy and classic culture method. The biofilms formed were not very dense and microscopic observations revealed uneven biofilm structures, with presence of exopolymeric matrix surrounding single cells, small aggregates and microcolonies, in a honeycomb-like arrangement. Moreover, planktonic population of L. monocytogenes (present in broth media covering the abiotic surface) remained stable throughout the incubation time, which indicates an efficient dispersal mechanism, since the culture medium was replaced daily. In conclusion, even if these strains of L. monocytogenes were not able to form thick multilayer biofilms, it was noticeable their high persistence on abiotic surfaces, reinforcing the need to focus on measures to avoid biofilm formation, instead of trying to eradicate mature biofilms.

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Section: Discussionsupporting

confidence: 76%

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces

Reis‐Teixeira

Alves

Martinis

2017

Brazilian Journal of Microbiology

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“…Of the L. monocytogenes isolates, strains 1698, 1707, 1720 and 1721 (all from the serogroup 1/2a-3a) were the best biofilm-forming strains. These strains formed significantly stronger biofilms at 15°C than the reference strains ScottA or EGDe, which were used for comparative evaluation of biofilm formation (Rieu et al 2008;Pilchov a et al 2014;Guilbaud et al 2015). Stronger biofilm formation by L. monocytogenes isolates from serogroup 1/2a-3a was also reported by Doijad et al (2015).…”

Section: Discussionmentioning

confidence: 97%

Inhibition ofListeria monocytogenesbiofilms by bacteriocin-producing bacteria isolated from mushroom substrate

et al. 2016

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“…Understanding the initial steps and structure of biofilm formation is important in terms of prevention and removal. In Listeria monocytogenes , the achievement of an initial 2‐D biofilm structure, observed using fluorescence microscopy (Pilchová et al ., ), can be divided into four steps: (i) cell cluster formation with attached cells side‐by‐side; (ii) branch elongation by cell division; (iii) open geometric forms connected through branches; and (iv) a honeycomb‐like structure. In this study, among the four test strains able to reach a honeycomb‐like structure, there was one (EGD‐e) which could not enter to the second step and subsequently could not develop complex biofilms.…”

Section: Development Of Biofilmsmentioning

confidence: 99%

“…In this study, among the four test strains able to reach a honeycomb‐like structure, there was one (EGD‐e) which could not enter to the second step and subsequently could not develop complex biofilms. Therefore, the biofilm primary structure appears to be an essential stage for the development of listerial biofilms with elaborated structure (Pilchová et al ., ).…”

Section: Development Of Biofilmsmentioning

confidence: 99%

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

Phillips

2016

Int J of Food Sci Tech

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Biofilms are immobile communities of micro-organisms attached to any surface, such as stainless steel or a food matrix surface or on packaging material. They may be composed of a single species, but more generally, in the natural environment, they consist of mixed species together with an extracellular matrix. Biofilms provide a common mechanism of persistence for a number of bacterial species especially in food processing environments, and therefore, prevention of biofilm formation and the removal of preformed biofilms are an important issue for the food industry. This article reviews the current understanding on the formation of biofilms and recent developments in biological and chemical methods for prevention and removal.

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Influence of food processing environments on structure initiation of static biofilm of Listeria monocytogenes

Cited by 54 publications

References 55 publications

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces

Inhibition ofListeria monocytogenesbiofilms by bacteriocin-producing bacteria isolated from mushroom substrate

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

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