Influence of Follicular Maturation on Progesterone Release in Chicken Granulosa Cells in Response to Turkey and Ovine Gonadotropins

Hammond, R.W.; Burke, W. H.; Hertelendy, F.

doi:10.1095/biolreprod24.5.1048

Cited by 95 publications

(28 citation statements)

References 18 publications

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“…Taken together, some of the discrepancies in the relative amount of FSH bioactivity (limited vs. none) in preovulatory follicle granulosa cells may be attributed to species differences within the FSH protein (e.g., ability to bind to the cFSH-R, as discussed above), variability in the purity of the FSH preparation (i.e., amount of contamination with LH [7]), and/or the stage of follicle development investigated [32]. For instance, mammalian species are generally proposed to contain three (e.g., bovine [24], rat [23], ovine [33]), or four (human [16,27], Macaca [28], equine [34]) glycosylation sites (important in peptide ligand recognition) within the extracellular binding domain of the FSH-R; yet only one of three glycosylation sites predicted within the cFSH-R amino acid sequence are conserved compared to the rat, ovine and bovine sequences.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Chicken Follicle-Stimulating Hormone Receptor (cFSH-R) Complementary Deoxyribonucleic Acid, and Expression of cFSH-R Messenger Ribonucleic Acid in the Ovary1

You

Bridgham

Foster

et al. 1996

Biology of Reproduction

122

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Studies were conducted to characterize the chicken (c) FSH receptor (R) cDNA, and to evaluate expression of cFSH-R mRNA in the hen ovary at known stages during follicle development. A total of 2.5 kb of nucleic acid sequence including the complete cFSH-R coding region was isolated by a combination of the reverse-transcription polymerase chain reaction and 5'- and 3'-rapid amplification of cDNA ends techniques. Overall, the nucleic acid sequence homology of the cFSH-R cDNA coding region is 71.8% and 72.2% compared to the rat and bovine FSH-R, respectively, while the deduced amino acid sequence identity for the receptor protein (693 amino acids) is 71.9% and 72.4%, respectively. By comparison, the cFSH-R nucleic acid and amino acid sequences are 60.1% and 49.4% identical to the respective cLH-R sequences. Northern blot analysis detected a single 4.3-kb cFSH-R mRNA transcript, which was selectively expressed in ovarian (granulosa, theca, and stromal) tissues, but not the oviduct, adrenal, liver, muscle, or brain. As the follicle developed from the prehierarchical (6- to 8-mm diameter) to the largest preovulatory (F1 follicle) stage, cFSH-R mRNA levels progressively declined within both the granulosa and theca layers (p < 0.05). Moreover, cFSH-R mRNA levels were lower in whole atretic than in morphologically normal 3- to 5-mm follicles (p = 0.0015). The pattern of cFSH-R mRNA expression within the granulosa layer during follicle development was notably different from that of the recently reported cLH-R, in that cLH-R mRNA levels increase to become readily detectable coincident with dramatically increased steroidogenic capacity during the last few days before ovulation of the follicle. On the other hand, highest levels of cFSH-R mRNA in 6- to 8-mm (prehierarchical) follicles were consistent with a role for the cFSH-R in maintaining the viability of prehierarchical follicles and in initiating granulosa cell differentiation at the time when follicles are selected into the preovulatory hierarchy.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the Chicken Follicle-Stimulating Hormone Receptor (cFSH-R) Complementary Deoxyribonucleic Acid, and Expression of cFSH-R Messenger Ribonucleic Acid in the Ovary1

You

Bridgham

Foster

et al. 1996

Biology of Reproduction

122

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“…While this decline in steroidogenesis (decreased C17-20 lyase and aromatase activity) has previously been attributed to the loss of LH-stimulated steroidogenesis in mature theca cells [28], the present data suggest that there may be a concomitant decrease in tLH-R intact expression. By comparison, in the chicken, the responsiveness of granulosa cells to LH stimulation, in terms of enhanced cAMP and steroidogenesis, showed a marked increase during the last few days of follicular maturation, peaking shortly after ovulation [29,30]. Also, the increased LH responsiveness of these cells during follicular maturation correlates well with significant increase in the tLHR intact expression.…”

Section: Discussionmentioning

confidence: 90%

Three Different Turkey Luteinizing Hormone Receptor (tLH-R) Isoforms II: Characterization of Differentially Regulated tLH-R Messenger Ribonucleic Acid Isoforms in the Ovary

You

Kim

Halawani

et al. 2000

Biology of Reproduction

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We have recently characterized three different, alternatively spliced, partial turkey LH receptor (tLH-R) cDNA isoforms by the combination of reverse transcription-polymerase chain reaction (RT-PCR) and 5'- and 3'-rapid amplification of cDNA ends. The first cDNA (intact form: tLH-R(intact)) showed 98% and 72-75% similarity with chicken and mammalian LH receptor sequences, respectively. The other two cloned cDNA isoforms (insertion and truncated forms: tLH-R(insert) and tLH-R(trunc)) could encode truncated soluble protein isoforms that lack the transmembrane region. Northern blot analysis detected two transcripts of 3.0 kilobases (kb) (tLH-R(intact)) and 1.5 kb (tLH-R(trunc)) in the turkey ovary but could not discriminate a third alternatively spliced transcript (tLH-R(insert)) due to the small 86-base pair difference in the size range of approximately 3.0-kb mRNAs. But with the combination of RNase protection assay, RT-PCR, and Northern blot analysis, three different alternatively spliced tLH-R mRNA isoforms were quantified. Differential expression of the tLH-R mRNA isoforms was demonstrated in ovarian stromal tissue during various reproductive stages and in the theca and granulosa layer through follicular development. To gain a better understanding of the physiological significance of the three different tLH-R isoforms, total RNA from the theca layer through follicular development after prolactin (PRL) treatment was analyzed by RT-PCR. PRL treatment for 8-14 days significantly increased the steady-state levels of total tLH-R mRNAs, including tLH-R(insert) and tLH-R(trunc) mRNAs, compared to those in nontreated controls. In contrast, the steady-state levels of tLH-R(intact) mRNA during the same period was not significantly changed when compared to that in nontreated controls. The present study shows that tLH-R transcripts are alternatively spliced in a tissue-specific manner in the turkey and that the mechanism may, in part, be controlled hormonally.

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“…Follicle stimulating hormone (FSH) also plays a major role in the ovulatory cycle, but the actions are less well defined than those of LH (Hammond, Burke, & Hertelendy, 1981). Like LH, FSH stimulates the production and release of progesterone from small yellow follicles.…”

Section: Hormone Regulation Of the Reproductive Systemmentioning

confidence: 99%

Salmonella Enteritidis in shell eggs: Current issues and prospects for control

Howard

O’Bryan

Crandall

et al. 2012

Food Research International

151

111

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Influence of Follicular Maturation on Progesterone Release in Chicken Granulosa Cells in Response to Turkey and Ovine Gonadotropins

Cited by 95 publications

References 18 publications

Characterization of the Chicken Follicle-Stimulating Hormone Receptor (cFSH-R) Complementary Deoxyribonucleic Acid, and Expression of cFSH-R Messenger Ribonucleic Acid in the Ovary1

Characterization of the Chicken Follicle-Stimulating Hormone Receptor (cFSH-R) Complementary Deoxyribonucleic Acid, and Expression of cFSH-R Messenger Ribonucleic Acid in the Ovary1

Three Different Turkey Luteinizing Hormone Receptor (tLH-R) Isoforms II: Characterization of Differentially Regulated tLH-R Messenger Ribonucleic Acid Isoforms in the Ovary

Salmonella Enteritidis in shell eggs: Current issues and prospects for control

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