Influence of fluoride concentration and ethanol pre-treatment on the reduction of the acid susceptibility of enamel

Wegehaupt, Florian J; Solt, Barbara; Sener, Beatrice; Wiegand, Annette; Schmidlin, Patrick R.; Attin, Thomas

doi:10.1016/j.archoralbio.2009.06.006

Cited by 12 publications

(11 citation statements)

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“…This method has been used in numerous other studies concerning dental hard tissue wear due to erosion [West et al, 2000], abrasion [Wegehaupt et al, 2010] or erosion/ abrasion [Attin et al, 2000a, b;Hooper et al, 2007]. Tooth wear has also been measured with other methods, such as assessment of the calcium diluted in the acid of the erosive attack [Wegehaupt et al, 2009b], calculating the wear due to the length change of previously applied Knoop indentations [Joiner et al, 2005;Wiegand et al, 2007] or measuring the amount of radioactive phosphate in the slurry after brushing radioactivated tooth structure [Imfeld, 2001]. These methods are used for the determination of tooth wear caused either by erosion or abrasion.…”

Section: Discussionmentioning

confidence: 99%

The Role of Fluoride and Casein Phosphopeptide/Amorphous Calcium Phosphate in the Prevention of Erosive/Abrasive Wear in an in vitro Model Using Hydrochloric Acid

Wegehaupt

Attin

2010

Caries Res

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Objective: To investigate the effect of various fluoride compounds and casein phosphopeptide/amorphous calcium phosphate (CPP-ACP) on the reduction of erosive/abrasive tooth wear. Methods: Forty enamel samples were prepared from bovine lower incisors, stratified and allocated to 4 groups (1–4). Samples in group 1 remained untreated and served as negative controls. The test samples were treated for 2 min/day as follows: group 2 amine/sodium fluoride gel (pH 4.8; 12,500 ppm), group 3 sodium fluoride gel (pH 7.1; 12,500 ppm) and group 4 CPP-ACP-containing mousse. De- and remineralization cycling was performed for 20 days with 6 erosive attacks for 20 s with HCl (pH 3.0) per day. Samples were stored in artificial saliva between cycles and overnight. Toothbrushing (15 s; 60 strokes/min; load 2.5 N) with a toothpaste slurry was performed each day before the first and 1 h after the last erosive exposure. Tooth wear was measured by comparing baseline surface profiles with the corresponding posttreatment profiles. Results: Tooth wear was significantly reduced in groups 2 and 3 compared with group 1, while the enamel loss of group 4 was not significantly lower compared to the negative control group 1. Between the fluoride groups 2 and 3, no significant difference in tooth wear was recorded. Conclusion: Erosive/abrasive tooth wear under the conditions used could be reduced significantly by the daily application of fluoride gels, irrespective of the fluoride compound, while the application of CPP-ACP-containing mousse was less effective.

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Section: Discussionmentioning

confidence: 99%

The Role of Fluoride and Casein Phosphopeptide/Amorphous Calcium Phosphate in the Prevention of Erosive/Abrasive Wear in an in vitro Model Using Hydrochloric Acid

Wegehaupt

Attin

2010

Caries Res

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“…As the measurement of the calcium dissolved in the acid of the acidic attack has been determined to be a sufficient method for the determination of mineral loss during erosion 33 and has been used in various other studies 13,14 , this method was also employed in the present study.…”

Section: Zhang Et Al (1999)mentioning

confidence: 99%

“…Since then numerous other in vitro and in situ studies have been performed to examine erosive tooth wear and its prevention, especially focussing on enamel [8][9][10][11][12][13] . Studies dealing with dentine as a substrate are scarce 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Application of cerium chloride to improve the acid resistance of dentine

Wegehaupt

Sener

Attin

et al. 2010

Archives of Oral Biology

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R (2010). Application of cerium chloride to improve the acid resistance of dentine. Archives of Oral Biology, 55(6): [441][442][443][444][445][446] Application of cerium chloride to improve the acid resistance of dentine Abstract OBJECTIVE: To investigate the effect of cerium chloride, cerium chloride/fluoride and fluoride application on calcium release during erosion of treated dentine. METHODS: Forty dentine samples were prepared from human premolars and randomly assigned to four groups (1-4). Samples were treated twice a day for 5 days, 30s each, with the following solutions: group 1 placebo, group 2 fluoride (Elmex fluid), group 3 cerium chloride and group 4 combined fluoride and cerium chloride. For the determination of acid resistance, the samples were consecutively eroded six times for 5 min with lactic acid (pH 3.0) and the calcium release in the acid was determined. Furthermore, six additional samples per group were prepared and used for EDS analysis. SEM pictures of these samples of each group were also captured. RESULTS: Samples of group 1 presented the highest calcium release when compared with the samples of groups 2-4. The highest acid resistance was observed for group 2. Calcium release in group 3 was similar to that of group 4 for the first two erosive attacks, after which calcium release in group 4 was lower than that of group 3. Generally, the SEM pictures showed a surface coating for groups 2-4. No deposits were observed in group 1. CONCLUSION: Although fluoride showed the best protective effect, cerium chloride was also able to reduce the acid susceptibility of dentine significantly, which merits further investigation. Key words: dentine, fluoride, cerium chloride, acid, erosion Application of cerium chloride to improve the acid resistance of dentine Abstract:Objective: To investigate the effect of cerium chloride, cerium chloride/fluoride and fluoride application on calcium release during erosion of treated dentine.Methods: Forty dentine samples were prepared from human premolars and randomly assigned to four groups (1 -4). Samples were treated twice a day for 5 days, 30s each, with the following solutions: group 1 placebo, group 2 fluoride (Elmex fluid), groups 3 cerium chloride and group 4 combined fluoride and cerium chloride. For the determination of acid resistance, the samples were consecutively eroded six times for 5 min with lactic acid (pH 3.0) and the calcium release in the acid was determined.Furthermore, six additional samples per group were prepared and used for EDS analysis. SEM pictures of these samples of each group were also captured.

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“…1,2 Although the efficacy of fluoride application in preventing dental erosion is still under debate, many studies have shown a protective effect of fluoride agents on erosion of enamel and dentine. [3][4][5][6][7][8][9] Recent work demonstrated that fluoride preparations containing stannous ions showing promising protective effects against cyclic erosive attacks in enamel. 3,10 After the application of a tincontaining fluoride solution, a relatively dense tin-rich layer was present on enamel surface, thus reducing the erosive substance loss.…”

Section: Introductionmentioning

confidence: 99%

Erosion-inhibiting potential of a stannous chloride-containing fluoride solution under acid flow conditions in vitro

Yu¹,

Wegehaupt²,

Zaruba³

et al. 2010

Archives of Oral Biology

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This study aimed to analyse the erosion-inhibiting potential of a single application of stannous chloride-containing fluoride solution on pellicle-covered enamel and dentine under constant acid flow conditions in vitro. DESIGN: Bovine enamel (n=60) and dentine (n=60) samples were exposed 1h to the oral cavity of 4 healthy volunteers to allow for in situ pellicle formation. Pellicle-covered samples were randomly assigned to three groups (each n=20 enamel and n=20 dentine samples; 5 enamel and 5 dentine samples/volunteer) and treated once with a SnCl2/AmF/NaF (800 ppm Sn(II), 500 ppm F, pH 4.5) or a NaF solution (500 ppm F, pH 4.5) for 2 min or remained untreated (controls). Samples were eroded with hydrochloric acid (pH 2.6) in a small erosion chamber at 60 microl/min for 25 min. Calcium release into the acid was monitored in consecutive 30s intervals for 5 min, then at 1 min intervals up to a total erosion time of 25 min using the Arsenazo III procedure. Data were statistically analysed by random-effects linear models (p<0.05). RESULTS: The stannous chloride-containing fluoride solution reduced calcium loss of enamel and dentine to up to 6 min and 3.5 min, respectively. Calcium loss (% of control) amounted from 24+/-7 (30s) up to 93+/-14 (6 min) in enamel and from 38+/-13 (30s) to 87+/-15 (3.5 min) in dentine. The sodium fluoride solution was unable to reduce enamel and dentine erosion at any time point. CONCLUSION: A single application of a stannous chloride-containing fluoride solution reduced enamel and dentine erosion up to 6 min and 3.5 min of constant acid flow, respectively. Design: Bovine enamel (n = 60) and dentine (n = 60) samples were exposed 1 h to the oral cavity of four healthy volunteers to allow for in situ pellicle formation. Pellicle-covered samples were randomly assigned to three groups (each n = 20 enamel and n = 20 dentine samples; 5 enamel and 5 dentine samples/ volunteer) and treated once with a SnCl 2 /AmF/NaF (800 ppm Sn(II), 500 ppm F, pH 4.5) or a NaF solution (500 ppm F, pH 4.5)for 2 min or remained untreated (controls). Samples were eroded with hydrochloric acid (pH 2.6) in a small erosion chamber at 60 µl/min for 25 min. Calcium release into the acid was monitored in consecutive 30 s intervals for 5 min, then at 1 min intervals up to a total erosion time of 25 min using the Arsenazo III procedure. Data were statistically analysed by randomeffects linear models (p < 0.05). Results:The stannous chloride-containing fluoride solution reduced calcium loss of enamel and dentine to up to 6 min and 3.5 min, respectively. Calcium loss (% of control) amounted from 24±7 (30 s) up to 93±14 (6 min) in enamel and from 38±13 (30 s) to 87±15 (3.5 min) in dentine. The sodium fluoride solution was unable to reduce enamel and dentine erosion at any time point. Conclusion:A single application of a stannous chloride-containing fluoride solution reduced enamel and dentine erosion up to 6 min and 3.5 min of constant acid flow, respectively.3

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Influence of fluoride concentration and ethanol pre-treatment on the reduction of the acid susceptibility of enamel

Cited by 12 publications

References 36 publications

The Role of Fluoride and Casein Phosphopeptide/Amorphous Calcium Phosphate in the Prevention of Erosive/Abrasive Wear in an in vitro Model Using Hydrochloric Acid

The Role of Fluoride and Casein Phosphopeptide/Amorphous Calcium Phosphate in the Prevention of Erosive/Abrasive Wear in an in vitro Model Using Hydrochloric Acid

Application of cerium chloride to improve the acid resistance of dentine

Erosion-inhibiting potential of a stannous chloride-containing fluoride solution under acid flow conditions in vitro

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