“…Authors of various articles were concerned with FFA analytical methods (Sjaunja, 1984;Koops et al, 1990;Foss, 2001Foss, , 2004Bijgaart, 2006;Hanuš et al, a, 2009) and above mentioned methodical complications ensued on these papers. Reference and routine FFA analytical methods can be so called extraction-titration method, churning-titration method, BDI and mid infrared spectroscopy (MIR) also in modifi cation with Fourier's transformations (MIR-FT; CSN 57 0533; Koops et al, 1990;IDF, 1991;Cvak et al, 1: Lipolysis rise of fat in raw milk, content increase of free fatty acids (FFA), threat of milk and dairy product quality -factors and their combinations, related to animal and technology (modified according to Sjaunja (1984), Shelley et al (1987), O'Brian et al (1998), , Ma et al (2000), Antonelli et al (2002), Santos et al (2003), Wiking et al (2003Wiking et al ( , 2006, Hanuš et al (2004, Thomson et al (2005), Ferlay et al (2006), Genčurová et al (2009) and Mikulová (2011 1992; Foss, 2001Foss, , 2004Thomson et al, 2005;Bijgaart, 2006;Mikulová, 2011).…”