Influence of extracellular matrix proteins on human keratinocyte attachment, proliferation and transfer to a dermal wound model

Dawson, Rebecca; Goberdhan, N.J.; Freedlander, E.; MacNeil, Sheila

doi:10.1016/0305-4179(95)00092-5

Cited by 62 publications

(39 citation statements)

References 29 publications

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“…Keratinocytes strongly depend on secreted factors and ECM produced from fibroblasts to properly differentiate into a stratified epidermis. [46][47][48] Thus, printed dermal models were cultured for up to 7 weeks and were seeded with human dermal keratinocytes at different time points to determine the optimal seeding procedure. In the printed dermal models, ECM expression increased throughout culture time as verified with collagen type I expression.…”

Section: Discussionmentioning

confidence: 99%

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

et al. 2016

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“…Each type of biomaterials has desirable traits which are exclusive of the other. Acellular tissue matrices possess the desired biocompatibility (11)(12)(13), contain biomimetic factors (14)(15)(16)) that promote tissue development and have adhesion domain sequences (e.g., RGD) that may assist in retaining the phenotype and activity of many types of cells (17). These matrices are known to slowly degrade upon implantation and are usually replaced and remodeled by ECM proteins synthesized and secreted by transplanted or ingrowing cells (18)(19)(20)(21)(22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Composite scaffolds for the engineering of hollow organs and tissues

Eberli¹,

Filho²,

Atala³

et al. 2009

Methods

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Composite scaffolds for the engineering of hollow organs and tissues AbstractSeveral types of synthetic and naturally derived biomaterials have been used for augmenting hollow organs and tissues. However, each has desirable traits which were exclusive of the other. We fabricated a composite scaffold and tested its potential for the engineering of hollow organs in a bladder tissue model. The composite scaffolds were configured to accommodate a large number of cells on one side and were designed to serve as a barrier on the opposite side. The scaffolds were fabricated by bonding a collagen matrix to PGA polymers with threaded collagen fiber stitches. Urothelial and bladder smooth muscle cells were seeded on the composite scaffolds, and implanted in mice for up to 4 weeks and analyzed. Both cell types readily attached and proliferated on the scaffolds and formed bladder tissue-like structures in vivo. These structures consisted of a luminal urothelial layer, a collagen rich compartment and a peripheral smooth muscle layer. Biomechanical studies demonstrated that the tissues were readily elastic while maintaining their pre-configured structures. This study demonstrates that a composite scaffold can be fabricated with two completely different polymer systems for the engineering of hollow organs. The composite scaffolds are biocompatible, possess adequate physical and structural characteristics for bladder tissue engineering, and are able to form tissues in vivo. This scaffold system may be useful in patients requiring hollow organ replacement. Title: COMPOSITE SCAFFOLDS FOR THE ENGINEERING OF HOLLOW ORGANS AND TISSUES Authors Abstract:Several types of synthetic and naturally derived biomaterials have been used for augmenting hollow organs and tissues. However, each has desirable traits which were exclusive of the other. We fabricated a composite scaffold and tested its potential for the engineering of hollow organs in a bladder tissue model. The composite scaffolds were configured to accommodate a large number of cells on one side and were designed to serve as a barrier on the opposite side. The scaffolds were fabricated by bonding a collagen matrix to PGA polymers with threaded collagen fiber stitches. Urothelial and bladder smooth muscle cells were seeded on the composite scaffolds, and implanted in mice for up to 4 weeks and analyzed. Both cell types readily attached and proliferated on the scaffolds and formed bladder tissue-like structures in vivo. These structures consisted of a luminal urothelial layer, a collagen rich compartment and a peripheral smooth muscle layer. Biomechanical studies demonstrated that the tissues were readily elastic while maintaining their pre-configured structures. This study demonstrates that a composite scaffold can be fabricated with two completely different polymer systems for the engineering of hollow organs. The composite scaffolds are biocompatible, possess adequate physical and structural characteristics for bladder tissue engineering, and are able to form tissues in vivo. Thi...

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“…During injury repair, the ECM proteins provide an acellular scaffold for epithelial cell attachment and cell-cell interactions (Dawson et al, 1996). Microscopic examination of the wounded skin tissues showed that skin injury caused an increase of intercellular spaces in the basal layer epithelial cells.…”

Section: Mekk1 Promotes Wounding Edge Epithelium Restorationmentioning

confidence: 99%

A Role for the Mitogen-activated Protein Kinase Kinase Kinase 1 in Epithelial Wound Healing

Deng

Chen

Takatori

et al. 2006

MBoC

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The mitogen-activated protein kinase kinase (MEK) kinase 1 (MEKK1) mediates activin B signals required for eyelid epithelium morphogenesis during mouse fetal development. The present study investigates the role of MEKK1 in epithelial wound healing, another activin-regulated biological process. In a skin wound model, injury markedly stimulates MEKK1 expression and activity, which are in turn required for the expression of genes involved in extracellular matrix (ECM) homeostasis. MEKK1 ablation or down-regulation by interfering RNA significantly delays skin wound closure and impairs activation of Jun NH 2 -terminal kinases, induction of plasminogen activator inhibitor (PAI)-1, and restoration of cell-cell junctions of the wounded epidermis. Conversely, expression of wild-type MEKK1 accelerates reepithelialization of full-thickness skin and corneal debridement wounds by mechanisms involving epithelial cell migration, a cell function that is partially abolished by neutralizing antibodies for PAI-1 and metalloproteinase III. Our data suggest that MEKK1 transmits wound signals, leading to the transcriptional activation of genes involved in ECM homeostasis, epithelial cell migration, and wound reepithelialization.

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Influence of extracellular matrix proteins on human keratinocyte attachment, proliferation and transfer to a dermal wound model

Cited by 62 publications

References 29 publications

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

Composite scaffolds for the engineering of hollow organs and tissues

A Role for the Mitogen-activated Protein Kinase Kinase Kinase 1 in Epithelial Wound Healing

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