A critical first step in establishing biosphere reserves-under the Man and Biosphere Programme of UNESCO-is to generate baseline information for future courses of action. The present study aims to assess the structure and composition of forests-along with anthropogenic pressures mounting on these forests in the buffer zone of one such biosphere reserves-the Pachmarhi biosphere reserve of India. The quadrat method was employed for sampling vegetation, and information on anthropogenic pressures was collected by conducting interviews with local people and forest officials and collecting it from secondary sources. A total of 39 tree species were sampled in 82 quadrats; of these 26 tree species were in standing stage, 25 in sapling, and 35 in seedling. Chloroxylon swietenia emerged as the most dominant tree species having highest importance value index, followed by Tectona grandis, Terminalia tomentosa, and Hardwickia binata. Nine tree species and their saplings, including Sterculia urens and Terminalia arjuna, were exploited so badly that they were only found in the seedlings stage. The unavailability of standing trees of 12 important tree species including Aegle marmelos and Phyllanthus emblica indicates the intensity and gravity of anthropogenic pressures on these important tree species. If the present anthropogenic pressure continues, which has inhibited the regeneration of several tree species, then substantial negative ecological and societal consequences can be expected.