Influence of excitation light intensity and leaf age on the slow chlorophyll fluorescence transient in radish

Nesterenko, T. V.; Tikhomirov, A. A.; Shikhov, V. N.

doi:10.1134/s0006350912040136

Cited by 6 publications

(8 citation statements)

References 22 publications

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“…The changes observed during the transition from P to S phase of the Kautsky kinetics indicate the actual proportion between the mechanisms involved in photochemical and non-photochemical quenching [36]. Since the parameters derived from the slow Kautsky kinetics responded to heat treatment, it might be concluded that they have a high potential in the evaluation of heat effects on the chloroplast function of tomato, as shown for light stress and leaf age effects by Nesterenko et al [37]. Many studies support the idea that a sustained increase in leaf photosynthesis can also lead to an increase in total biomass production [38].…”

Section: Discussionmentioning

confidence: 95%

Eco-Physiological Screening of Different Tomato Genotypes in Response to High Temperatures: A Combined Field-to-Laboratory Approach

Arena

Conti

Francesca

et al. 2020

Plants

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High temperatures represent a limitation for growth and development of many crop species. Several studies have demonstrated that the yield reduction of tomato under high temperatures and drought is mainly due to a photosynthetic decline. In this paper, a set of 15 tomato genotypes were screened for tolerance to elevated temperatures by cultivating plants under plastic walk-in tunnels. To assess the potential tolerance of tomato genotypes to high temperatures, measurements of chlorophyll fluorescence, pigments content and leaf functional traits have been carried out together with the evaluation of the final yields. Based on the greenhouse trials, a group of eight putative heat-sensitive and heat-tolerant tomato genotypes was selected for laboratory experiments aimed at investigating the effects of short-term high temperatures treatments in controlled conditions. The chlorophyll fluorescence induction kinetics were recorded on detached leaves treated for 60 min at 35 • C or at 45 • C. The last treatment significantly affected the photosystem II (PSII) photochemical efficiency (namely maximum PSII quantum efficiency, F v /F m, and quantum yield of PSII electron transport, Φ PSII ) and the non-photochemical quenching (NPQ) in the majority of genotypes. The short-term heat shock treatments also led to significant differences in the shape of the slow Kautsky kinetics and its significant time points (chlorophyll fluorescence levels minimum O, peak P, semi-steady state S, maximum M, terminal steady state T) compared to the control, demonstrating heat shock-induced changes in PSII functionality. Genotypes potentially tolerant to high temperatures have been identified. Our findings support the idea that chlorophyll fluorescence parameters (i.e., Φ PSII or NPQ) and some leaf functional traits may be used as a tool to detect high temperatures-tolerant tomato cultivars.Plants 2020, 9, 508 2 of 16 and quality [5,6]. Tomato (Solanum lycopersicum), being an excellent source of health-promoting compounds, is one of the most important crops cultivated worldwide and its heat sensitivity varies among different genotypes [4,7,8]. Generally, the optimal temperature range for photosynthesis is considered to be between 25 • C and 30 • C [6]. The rising of average temperatures due to the ongoing climate change will cause extensive productivity losses in Mediterranean areas, where tomato is traditionally cultivated [9][10][11]. In this framework, it becomes important to perform studies that are able to identify the most promising genotypes able to face heat stress.The relationship between gas exchange and crop yield has been largely studied in tomato, suggesting leaf transpiration as the most reliable indicator for yield prediction under drought [12]. However, beside gas exchange, other photosynthesis related parameters [13] should be taken into account to build a "eco-physiological identity card" for different genotypes.Chlorophyll fluorescence represents a good tool to rapidly and accurately detect plant health status, the occurrence of...

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Section: Discussionmentioning

confidence: 95%

Eco-Physiological Screening of Different Tomato Genotypes in Response to High Temperatures: A Combined Field-to-Laboratory Approach

Arena

Conti

Francesca

et al. 2020

Plants

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“…The second approach consists in careful adjustment of experimental conditions, especially actinic PFD, in order to minimize the influence of leaf age factor. For example, Nesterenko et al [26] found that age dependent variability of CFI ampli tude and temporal parameters was largely reduced at actinic irradiances above 200 µmol/(m 2 s) PAR. Table 1 suggest that, at actinic PFD of 380 µmol/(m 2 s), the age dependent condition of the leaf PSA cannot be determined with sufficient accuracy based on individual fluorescence parameters or their combination.…”

Section: Discussionmentioning

confidence: 99%

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

Nesterenko

Shikhov

Tikhomirov

2015

Russ J Plant Physiol

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Wheat (Triticum sativus L.) seedlings of various ages (2 to 16 day old plants) were used to study age dependent changes in the chlorophyll fluorescence induction (CFI) at various light intensities during flu orescence measurements. Plants were raised in a growth chamber using hydroponics with expanded clay, con trolled environmental conditions, and 690 µmol/(m 2 s) photon flux density (PFD) of photosynthetically active radiation (PAR). Parameters of CFI were determined under actinic PFD of 380, 580, 820, and 1340 µmol/(m 2 s) PAR. The fifth leaf from the stem base, exposed to uniform lighting, was sampled for mea surements. This leaf emerged at the plant age of 16 days. Based on fluorescence data, we calculated the max imal photochemical quantum yield of photosystem II (F v /F m ), the effective photochemical quantum yield of PSII (Yield), parameters of photochemical (qP) and non photochemical (qN and NPQ) quenching of chlo rophyll fluorescence, the F p /F t ratio, and the "vitality index" (fluorescence decrease ratio, R fd ). At moderate actinic PFD, applied commonly in PAM fluorometers (about 380 µmol/(m 2 s)), age dependent changes in NPQ, F p /F t , and R fd were observed. Analysis of CFI parameters in wheat leaves of different ages at PFD increasing from 380 to 820 µmol/(m 2 s) revealed that R fd , NPQ, and qN are the most sensitive markers of the leaf age among all parameters tested. These suitable indicators can be used for rapid assessment of the leaf age.

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“…(2) the parameter must remain sufficiently stable under widely varying fluorescence-exciting light -actinic light (AL) -intensity (Nesterenko et al 2012(Nesterenko et al , 2015; and (3) the parameter must be sensitive to changes in PSA activity under stress and recovery of the PSA state once the stress ceases (Li et al 2013, Shikhov et al 2016. Nesterenko et al (2014Nesterenko et al ( , 2015 studied age dependence of the main characteristics of CFI and the effect of AL intensity on their values in wheat (Triticum aestivum L.) leaves. The authors determined such para meters as quantum yield in PSII (Fv/Fm), effective quantum yield in PSII (ΦPSII); qP, qN, and NPQ -parameters of photochemical and nonphotochemical quenching of Chl fluorescence; the FP/Ft ratio; and vitality index Rfd.…”

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confidence: 99%

“…Kinetics of fluorescence intensity decrease in the light is often nonmonotonic, and, thus, it cannot be described by a sum of exponential curves. Therefore, we and other researchers used a formal parameter -τ0.5, an integrated parameter of the activation rates of certain photoassimilative and photoprotective processes in the leaf, as a temporal parameter of the CFI curve (Nesterenko and Siďko 1993, Kharuk et al 1994, Šesták and Šiffel 1997, Nesterenko et al 2012, Ptushenko et al 2013.…”

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confidence: 99%

“…A previous study showed that an increase in the intensity of the fluorescence-exciting light from 20 to 80 W m -2 [PPFD from 90 to 370 μmol(photon) m -2 s -1 ] did not cause any significant changes in the τ0.5 parameter of the CFI curves for a mature 18-d-old radish leaf (Nesterenko et al 2012). Analysis of the age dependence of τ 0.5 showed that it remained relatively stable over a certain period of time, which corresponded to the maturity stage of the leaf (Kharuk et al 1994, Šesták and Šiffel 1997, Nesterenko et al 2006.…”

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BRIEF COMMUNICATIONEstimation of changes in the activity of photosynthetic apparatus of plant leaves based on half-time of fluorescence intensity decrease

Nesterenko¹,

Shikhov²,

Tikhomirov³

2019

Photosynt.

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The range of variations in parameter τ0.5 -half-time of fluorescence intensity decrease during the slow phase of chlorophyll fluorescence induction (CFI) -has been studied during ontogeny of leaves of monocotyledonous and dicotyledonous plants in plant communities of different structures. Plants were grown hydroponically on expanded clay aggregate in growth chambers, under PPFD of 400 μmol(photon) m -2 s -1 , under controlled conditions. Analysis of the literature data and results of experimental observations of τ0.5 behavior compared to other CFI parameters, nonphotochemical quenching coefficient, qN, in particular, leads to the conclusion that parameter τ0.5 can be effectively used for indirect estimation of variations in the activity of photosynthetic apparatus during ontogeny of plant leaves.

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Influence of excitation light intensity and leaf age on the slow chlorophyll fluorescence transient in radish

Cited by 6 publications

References 22 publications

Eco-Physiological Screening of Different Tomato Genotypes in Response to High Temperatures: A Combined Field-to-Laboratory Approach

Eco-Physiological Screening of Different Tomato Genotypes in Response to High Temperatures: A Combined Field-to-Laboratory Approach

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

BRIEF COMMUNICATIONEstimation of changes in the activity of photosynthetic apparatus of plant leaves based on half-time of fluorescence intensity decrease

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