Influence of ethylene glycol on Eucalyptus grandis cryopreservation using the V cryo-plate technique

Oliveira, Kamila Ellen Souza de; Souza, Rafaeli Aparecida Vieira de; Carvalho, Lara Siqueira Oliveira; Paiva, Luciano Vilela

doi:10.1590/1984-70332022v22n2a20

Cited by 3 publications

(2 citation statements)

References 38 publications

(41 reference statements)

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“…Cryopreservation of germplasm of plant material in liquid nitrogen guarantees long-term preservation of the material and at the same time reduces the availability of the occurrence of genetic changes possible in in vitro culture at positive temperatures (Jenderek and Reed, 2017;Kalaiselvi et al, 2017;Kaya et al, 2017). The technique of cryopreservation of plant tissues includes several stages (Burritt, 2012;Wolkers and Oldenhof, 2015;Linde et al, 2018;Oliveira et al, 2022). Primary explants are selected, isolated, sterilized from saprophytic microflora and aseptic plants are obtained in vitro, then optimal nutrient media, cultivation conditions for the growth and development of the explant are selected and multiplied to a sufficient amount for cryofreezing (Hamill and Rames, 2016).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of berry crops for creation of germplasm cryobanks

et al. 2024

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One of the main stages of cryopreservation of meristematic tissues in vegetative plants is a clonal micropropagation, which includes isolating the explants of the raw material in vitro and optimizing the culture medium for micropropagation. As the result of our studies, the optimal periods for in vitro micropropagation are: first - isolation of explants from initiated shoots of dormant buds (blackcurrants and raspberries) in January-March; the second - from actively growing shoots (blackcurrants and raspberries) in May-June, from the formed mustache (strawberry) in July-August. The optimal drugs for sterilization of raspberry explants are: a) 0.1% HgCl2 (6 min), then 3% H2O2 (15 min); b) chlorine-containing bleach «Domestos» in the dilution of H2O 1:9 (10 min). For blackcurrant: a) 0.1% HgCl2 (5 min) in combination with 0.1% fungicide “Topaz” (30 min); b) 0.1% HgCl2 (5 min) in combination with the treatment with KMnO4 (30 min); c) “Domestos” in the dilution of H2O 1:5 (20 min). For strawberry: a) 0.1% HgCl2 (6 min) followed by treatment with 3% H2O2 10 (min); b) 1% deochlor (7 min), 3% H2O2 (10 min); c) “Domestos” in the dilution of H2O 1:5 (8 min) with subsequent treatment 0,1% HgCl2 -7 min, then 0,20 mg/l КМnO4 - 30 min. Optimal compositions of culture media for micropropagation of blackcurrant - Murashige and Skoog (MS) medium with 0.5 mg L-1 BAP, 0.5 mg L-1 GA3, 0.1 mg L-1 IBA and 20 g L-1 glucose. For raspberry -MS medium with 0.5 mg L-1 BAP, 0.1 mg L-1 IBA, 10 mg L-1 iron chelate and 30 g L-1 sucrose. For strawberry - MS medium with 0.3 mg L-1 BAP, 0.01 mg L-1 IBA, 0.2 mg L-1 GA3, 10 mg L-1 iron chelate and 30 g L-1 sucrose. Based on these studies, the cryobank was created, which include the germplasm of in vitro meristematic tissues in 66 cultivars, hybrids and wild-growing forms of blackcurrant, raspberry and strawberry. Therefore, the aim of the research was to obtain aseptic plants, clonal micropropagation and the creation of a cryogenic collection of germplasm based on the developed technology.

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Section: Introductionmentioning

confidence: 99%

Micropropagation of berry crops for creation of germplasm cryobanks

et al. 2024

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“…Encapsulation/dehydration [16] and vitrification methods [17] are the most commonly used cryogenic methods. High applicability and rapid implementation of the vitrification technique have led to numerous variations of this method [18][19][20][21][22]. Despite the numerous advantages of cryopreservation, only few available viable protocols can guarantee good regeneration rates of the genetic material, therefore limiting application of cryopreservation for long-term germplasm conservation [23].…”

Section: Introductionmentioning

confidence: 99%

Development of Cryopreservation Technique for Meristems of Syringa vulgaris L. Cultivars

Королева

Molkanova

Vysotskaya

2023

IJPB

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Cryopreservation is considered to be one of the most effective methods for long-term storage of plant genetic resources, particularly for ornamental species. However, there is a very little research on cryopreservation of lilacs. In this study, for the first time the cryopreservation protocol (a variation of a pregrowth-dehydration method) was successfully applied to two cultivars of Syringa vulgaris: ‘Aucubaefolia’ and ‘Polina Osipenko’. Explants of both cultivars were able to withstand the different steps of the protocol, and high survival and regrowth percentages were obtained after exposure to liquid nitrogen (67–100% and 63–88%, respectively). The current study is mainly focused on the preculture conditions of the applied method. Based on our results, we propose the use of paclobutrazol (PBZ) with the combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the preculture medium for increasing explant tolerance to subsequent dehydration and freezing. During post-LN recovery, the explants appeared morphologically normal, and after 12–16 weeks after thawing, they were propagated and cultured as normal plantlets. Therefore, the reported method is effective for long-term storage of lilac meristems and could be used to create a cryobank of achievements in lilac breeding.

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Tissue Culture Innovations for Propagation and Conservation of Myrteae—A Globally Important Myrtaceae Tribe

Bao,

O’Donohue,

Sommerville

et al. 2024

Plants

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Myrteae is the most species-rich tribe in the Myrtaceae family, represented by a range of socioeconomically and ecologically significant species. Many of these species, including commercially relevant ones, have become increasingly threatened in the wild, and now require conservation actions. Tissue culture presents an appropriate in vitro tool to facilitate medium-term and long-term wild germplasm conservation, as well as for commercial propagation to maintain desirable traits of commercial cultivars. So far, tissue culture has not been extensively achieved for Myrteae. Here, tissue culture for Eugenia, one of the most species-rich genera in Myrteae, is reviewed, giving directions for other related Myrteae. This review also focuses on ex situ conservation of Australian Myrteae, including using seed banking and field banking. Despite some progress, challenges to conserve these species remain, mostly due to the increasing threats in the wild and limited research. Research into in vitro methods (tissue culture and cryopreservation) is paramount given that at least some of the species are ‘non-orthodox’. There is an urgent need to develop long-term in vitro conservation for capturing the remaining germplasm of threatened Myrteae.

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Influence of ethylene glycol on Eucalyptus grandis cryopreservation using the V cryo-plate technique

Cited by 3 publications

References 38 publications

Micropropagation of berry crops for creation of germplasm cryobanks

Micropropagation of berry crops for creation of germplasm cryobanks

Development of Cryopreservation Technique for Meristems of Syringa vulgaris L. Cultivars

Tissue Culture Innovations for Propagation and Conservation of Myrteae—A Globally Important Myrtaceae Tribe

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