Influence of Escherichia Coli on Urease-Induced Crystallisation in Human Urine

Abstract

Urease was added to urines inoculated with Escherichia coli 24 hours earlier and to control urines not inoculated with E. coli. The inoculation did not change the concentration of the measured urine components. The urease-induced ammonium ion production and pH increase was reduced in E. coli-inoculated urines compared to control urines. This suggests that E. coli can inhibit urease. The precipitation of both phosphate and magnesium on glass rods inserted in the urine was reduced with 40-50% in the E. coli-inoculated urines. The results demonstrate that E. coli can influence urease-induced crystallisation.

Impact of citrate therapy on the circadian rhythm of urinary magnesium ammonium phosphate saturation in normal individuals

“…11,12 Escherichia coli is not a urease producer; however, E. coli was found to reduce urine citrate and was speculated to influence ureaseinduced crystalization. 13 The present study also showed that the magnesium ammonium phosphate saturation was significantly decreased between 08.00 and 20.30 h on day 5 in comparison with day 1, despite the 3 g t.i.d. regimen.…”

The Role of Escherichia coliform in the Biomineralization of Calcium Oxalate Crystals

“…The common bacterium Escherichia coliform ( E. coli ) is often found in urinary stones and it could damage the protective anti‐adhesive GAG layer and cause crystal retention or alter urine composition in such a way that favors stone formation 26,27. It has also been found that E. coli plays an important role in urinary‐tract infections 28.…”