Influence of Epidermal Growth Factor with Cysteamine on in -Vitro Buffalo Embryo Development

El-Naby, Al-Shimaa Al-H H; M., Karima Ghoneimy; Scholkamy, T. H.; Sosa, Gamal A.; El-Roos, Mahmoud Abou

doi:10.21608/ejvs.2016.1080

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Cited by 2 publications

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“…A group of embryos without PES was left as a control. After 72 h of culture, cleavage was determined, and embryos were counted on day 7 (El-Naby et al, 2016).…”

Section: Advances Inmentioning

confidence: 99%

Changes in the Relative Abundance of miR-205, miR-26a-5p, let-7b and their Target Genes in Vitrified Bovine Embryos after Phenazine Ethosulfate Supplementation

Saber¹,

Ibrahim²,

Mahmoud³

et al. 2021

AAVS

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“…A group of embryos without PES was left as a control. After 72 h of culture, cleavage was determined, and embryos were counted on day 7 (El-Naby et al, 2016).…”

Section: Advances Inmentioning

confidence: 99%

Changes in the Relative Abundance of miR-205, miR-26a-5p, let-7b and their Target Genes in Vitrified Bovine Embryos after Phenazine Ethosulfate Supplementation

Saber¹,

Ibrahim²,

Mahmoud³

et al. 2021

AAVS

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Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro

Yousef

Hussein

Badr³

et al. 2018

SVU-International Journal of Veterinary Sciences

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The present study aims to study the effects of epidermal growth factors (EGF) supplementations to the in vitro maturation (IVM) medium of buffalo's oocytes on the oocyte maturation rate and embryo development competence. The ovaries were collected from El-Monieb slaughters house and transferred directly to the laboratory for the experiment processing. Excellent and good cumulus oocytes complexes (COCs) were aspirated from 3-8 mm diameter follicles. TCM-199 medium were used for in vitro maturation. In experiment I, COCs were incubated in EGF-containing IVM medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs at 38.5°C in humidified environment; 5% CO2 and 95% humidity. In experiment II, COCs were incubated in standard in vitro fertilization (IVF) medium with the same concentrations for 24 hrs in the same condition. In experiment III, COCs were incubated in standard in vitro culture (IVC) medium with the same concentrations for 24 hrs in the same condition. According to the expansion of oocytes and attaining the developmental embryo stages of morula and blastocyst, our results have shown that addition of 20 ng/ml of EGF to the IVM medium significantly increased the oocyte maturation and fertilization rates compared to those of CTL (76.96 ± 9.04 vs. 51.3 ± 4.66 and 67.96 ± 2.76 vs. 42.03 ± 4.83 %, respectively) (P<0.05). On the other hand, Addition of 20 ng/ml of EGF to the IVM medium significantly increased the fertilization rates compared to other concentrations (10 and 50 ng/ml) (67.96 ± 02.76 vs. 49.43 ± 03.67 and 34.70 ± 01.40 %, respectively). However, the developmental rates to morula and blastocyst stages in response to addition to IVC medium variably increased rather than those of control despite no significance.

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Influence of Epidermal Growth Factor with Cysteamine on in -Vitro Buffalo Embryo Development

Abstract: There were many growth factors as insulin growth factors (IGFs), epidermal growth factor (EGF), transforming growth factor α, β and activin acted as F AL-SHIMAA A.H. EL-NABY et al.

Cited by 2 publications

References 49 publications

Changes in the Relative Abundance of miR-205, miR-26a-5p, let-7b and their Target Genes in Vitrified Bovine Embryos after Phenazine Ethosulfate Supplementation

Changes in the Relative Abundance of miR-205, miR-26a-5p, let-7b and their Target Genes in Vitrified Bovine Embryos after Phenazine Ethosulfate Supplementation

Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro

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