Influence of environmental factors in the production of R(?)-1, 2-propanediol by clostridium thermosaccharolyticum

Sanchez‐Riera, Fernando; Cameron, Douglas C.; Cooney, Charles L.

doi:10.1007/bf01027450

Cited by 40 publications

(22 citation statements)

References 10 publications

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“…Furthermore, studies on the conversion of sugars to 1,2-PDO have markedly lower yields than 1,3-PDO production from either sugars or glycerol [20]. This is in large part due to the fact that few microorganisms have been shown to naturally produce 1,2-PDO in large amounts, and those that have, such as Thermoanaerobacterium thermosaccharolyticum [44], lack the sufficient genetic knowledge and tools required for effective manipulation. In addition, when considering 1,2-PDO production form glycerol, it is important to note that the typical fermentative pathways for 1,2-PDO production require the conversion of the carbon source to dihydroxyacetone phosphate (DHAP) through glycolytic pathways, in contrast to the 1,3-PDO pathways that convert glycerol directly to 1,3-PDO in two-steps (Figure 4), which requires that the organism of interest possess the ability to utilize glycerol directly.…”

Section: Trends In Biotechnologymentioning

confidence: 93%

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals

Clomburg

González

2013

Trends in Biotechnology

271

184

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Section: Trends In Biotechnologymentioning

confidence: 93%

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals

Clomburg

González

2013

Trends in Biotechnology

271

184

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“…Recent processes for the microbial production of 1,2-PDO from sugars, predominantly glucose, have used microorganisms such as Thermoanaerobacterium thermosaccharolyticum (Altaras et al, 2001;Sanchez-Riera et al, 1987), Clostridium sphenoides (Trandin and Gottschalk, 1985), Saccharomyces cerevisiae (Jung et al, 2008;Lee and DaSilva, 2006), and E. coli Cameron, 1999, 2000;Huang et al, 1999). Utilizing batch cultures, 1,2-PDO titers from glucose ranged from 0.49 g/L with E. coli (Altaras and Cameron, 1999) to 1.11 g/L with S. cerevisiae (Jung et al, 2008) and 9.0 g/L with T. thermosaccharolyticum (Sanchez-Riera et al, 1987). An optimized fed-batch culture was used to achieve the highest titer of 1,2-PDO produced by E. coli from glucose (4.5 g/L) at a yield of 0.19 g/g (Altaras and Cameron, 2000).…”

Section: Introductionmentioning

confidence: 97%

Metabolic engineering of Escherichia coli for the production of 1,2‐propanediol from glycerol

Clomburg

González

2010

Biotech & Bioengineering

121

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Due to its availability, low-price, and high degree of reduction, glycerol has become an attractive carbon source for the production of fuels and reduced chemicals. Using the platform we have established from the identification of key pathways mediating fermentative metabolism of glycerol, this work reports the engineering of Escherichia coli for the conversion of glycerol into 1,2-propanediol (1,2-PDO). A functional 1,2-PDO pathway was engineered through a combination of overexpression of genes involved in its synthesis from the key intermediate dihydroxyacetone phosphate (DHAP) and the manipulation of the fermentative glycerol utilization pathway. The former included the overexpression of methylglyoxal synthase (mgsA), glycerol dehydrogenase (gldA), and aldehyde oxidoreductase (yqhD). Manipulation of the glycerol utilization pathway through the replacement of the native E. coli PEP-dependent dihydroxyacetone kinase (DHAK) with an ATP-dependent DHAK from C. freundii increased the availability of DHAP allowing for higher 1,2-PDO production. Analysis of the major fermentative pathways identified ethanol as a required co-product while increases in 1,2-PDO titer and yield were achieved through the disruption of the pathways for acetate and lactate production. Combination of these key metabolic manipulations resulted in an engineered E. coli strain capable of producing 5.6 g/L 1,2-PDO, at a yield of 21.3% (w/w). This strain also performed well when crude glycerol, a by-product of biodiesel production, was used as the substrate. The titer and yield achieved in this study were favorable to those obtained with the use of E. coli for the production of 1,2-PDO from common sugars.

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“…The best substrate for 1,2-PD formation was glucose. The glucose fermentation was further investigated by Sanchez-Riera et al (1987) and fermentation conditions were optimized to obtain a 1,2-PD yield of 0.20 g per gram of glucose consumed.…”

Section: Introductionmentioning

confidence: 99%

Conversion of Sugars to 1,2-Propanediol by Thermoanaerobacterium thermosaccharolyticum HG-8

2001

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The purpose of this study was to explore a fermentation route for the production of 1,2-propanediol (1,2-PD) from renewable sugars: lactose found in cheese whey, and D-glucose, D-galactose, L-arabinose, and D-xylose found in corn and wood byproducts. Thermoanaerobacterium thermosaccharolyticum, a naturally occurring organism, was found to ferment a wider range of sugars to 1,2-PD than previously reported. The specific sugar had a significant effect on the selectivity for 1,2-PD vs other fermentation products such as ethanol, D- and L-lactate, and acetate. T. thermosaccharolyticum potentially provides an environmentally friendly route to a major commodity chemical now made from petrochemicals.

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Influence of environmental factors in the production of R(?)-1, 2-propanediol by clostridium thermosaccharolyticum

Cited by 40 publications

References 10 publications

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals

Metabolic engineering of Escherichia coli for the production of 1,2‐propanediol from glycerol

Conversion of Sugars to 1,2-Propanediol by Thermoanaerobacterium thermosaccharolyticum HG-8

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