Influence of enamel/dentin thickness on the toxic and esthetic effects of experimental in-office bleaching protocols

Duque, Carla Caroline de Oliveira; Soares, Diana Gabriela; Basso, Fernanda Gonçalves; Hebling, Josimeri; Costa, Carlos Alberto de Souza

doi:10.1007/s00784-017-2049-7

Cited by 62 publications

(80 citation statements)

References 21 publications

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“…Therefore, to achieve effective dental color alteration in short periods, highly concentrated H 2 O 2 bleaching gels (35-40%) have been traditionally used for professional tooth-bleaching. 1 However, many studies have shown that such esthetic therapy allows diffusion of high amounts of H 2 O 2 through enamel and dentin, 4,5 causing in vitro [6][7][8][9][10][11] and in vivo [12][13][14][15] toxicity to pulp cells. Therefore, the "non-reacted H 2 O 2 " has been considered the main pathway for bleaching-induced tooth sensitivity, claimed by 80-100% of patients undergoing professional tooth-bleaching performed with high-concentrated gels.…”

Section: Introductionmentioning

confidence: 99%

“…18 Several authors have reported that reducing the H 2 O 2 concentration in bleaching gels and the period of contact of these dental products with enamel may decrease the in vitro and in vivo toxicity caused by these esthetic therapies. [7][8][9][10][12][13][14][15]22 However, additional sessions are needed to achieve bleaching outcomes similar to those of traditional therapies. 7,10,23 Increasing the degradation rate of H 2 O 2 has also been proposed as an alternative to minimizing residual H 2 O 2 diffusion through enamel and dentin.…”

Section: Introductionmentioning

confidence: 99%

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Increased whitening efficacy and reduced cytotoxicity are achieved by the chemical activation of a highly concentrated hydrogen peroxide bleaching gel

et al. 2019

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Objective: This study was designed for the chemical activation of a 35% hydrogen peroxide (H 2 O 2) bleaching gel to increase its whitening effectiveness and reduce its toxicity. Methodology: First, the bleaching gel-associated or not with ferrous sulfate (FS), manganese chloride (MC), peroxidase (PR), or catalase (CT)-was applied (3x 15 min) to enamel/dentin discs adapted to artificial pulp chambers. Then, odontoblast-like MDPC-23 cells were exposed for 1 h to the extracts (culture medium + components released from the product), for the assessment of viability (MTT assay) and oxidative stress (H 2 DCFDA). Residual H2O2 and bleaching effectiveness (DE) were also evaluated. Data were analyzed with one-way ANOVA complemented with Tukey's test (n=8. p<0.05). Results: All chemically activated groups minimized MDPC-23 oxidative stress generation; however, significantly higher cell viability was detected for MC, PR, and CT than for plain 35% H2O2 gel. Nevertheless, FS, MC, PR, and CT reduced the amount of residual H2O2 and increased bleaching effectiveness. Conclusion: Chemical activation of 35% H2O2 gel with MC, PR, and CT minimized residual H2O2 and pulp cell toxicity; but PR duplicated the whitening potential of the bleaching gel after a single 45-minute session.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Increased whitening efficacy and reduced cytotoxicity are achieved by the chemical activation of a highly concentrated hydrogen peroxide bleaching gel

et al. 2019

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“…Furthermore, we were able to determine a time point for cell recovery of 24 h for Zoom ® and 48 h for Zoom ® + irradiation. Previous studies also reported cell recovery at 72 h for other whitening products, so we hypothesize that despite different brand compositions, the presence of agents in the composition of products is responsible for cell protection [10,34]. Although we did not examine the effect of individual ingredients in Zoom ® composition, present compounds as glycerin and some polymers make the product thicker, decreasing the release of ROS from the product, which can explain these results.…”

Section: Discussionmentioning

confidence: 79%

Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts

et al. 2020

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Tooth whitening procedures are increasing; however, side effects can occur, such as damage to pulp cells, by the whitening products. This study aims to assess the cellular effects promoted by a whitening product, namely, the oxidative stress fostered by the active agent hydrogen peroxide, with and without photoactivation. Additionally, if cellular recovery occurred, we intended to determine the time point where cells recover from the tooth whitening induced damage. Human fibroblasts were exposed to hydrogen peroxide, Zoom®, Zoom® + irradiation, and irradiation alone. The following analysis was performed: metabolic activity evaluation by the MTT assay; cell viability, mitochondrial membrane potential, peroxides production, superoxide radical production, and reduced glutathione expression by flow cytometry. We determined the IC50 value for all groups, and a dose-dependent cytotoxic effect was verified. At the times analyzed, hydrogen peroxide groups showed no metabolic activity recovery while a cell recovery was observed after 24 h (Zoom®) and 48 h (Zoom® + irradiation). Cell death was seen in hydrogen peroxide and Zoom® + irradiation groups, mainly by apoptosis, and the irradiation had a cytotoxic effect per se. This in vitro study supports that whitening products with moderate hydrogen peroxide (HP) concentration have a temporary effect on cells, allowing a cellular recovery.

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“…Low cytotoxicity and safe use have been previously reported. Solutions of 6% and 10% hydrogen peroxide showed no cytotoxicity to MDPC‐23 odontoblast‐like cells and pulp cells. Additionally, a clinical study reported that exposures to hydrogen peroxide 3% by oral route are usually asymptomatic and have benign outcomes.…”

Section: Discussionmentioning

confidence: 98%

Control of bacterial biofilms formed on pacifiers by antimicrobial solutions in spray

Pedroso

Sangalli

Brighenti

et al. 2018

Int J Paed Dentistry

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Influence of enamel/dentin thickness on the toxic and esthetic effects of experimental in-office bleaching protocols

Abstract: Bleaching gels with 10% HO applied in small teeth for short periods may be an interesting alternative to obtain whitening effectiveness without causing toxicity to pulp cells, which may be able to reduce the tooth hypersensitivity claimed by patients.

Cited by 62 publications

References 21 publications

Increased whitening efficacy and reduced cytotoxicity are achieved by the chemical activation of a highly concentrated hydrogen peroxide bleaching gel

Increased whitening efficacy and reduced cytotoxicity are achieved by the chemical activation of a highly concentrated hydrogen peroxide bleaching gel

Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts

Control of bacterial biofilms formed on pacifiers by antimicrobial solutions in spray

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