Influence of elevated temperature on bovine oviduct epithelial cells (BOECs)

Rapala, L.; Starzyński, Rafał R.; Trzeciak, P.; Dąbrowski, Sebastian; Gajewska, Małgorzata; Jurka, P.; Smolarczyk, Roman; Duszewska, A. M.

doi:10.1371/journal.pone.0198843

Cited by 8 publications

(6 citation statements)

References 52 publications

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“…Bovine oviduct epithelial cells exposed to heat stress in vitro also showed increased secretion of PGE 2 [57], an important factor for oviduct motility. In another study, long-term (168 h) exposure of bovine oviduct epithelial cells to high temperature (41 • C) increased the expression of HSP70 with no effect on cell viability [107], in accordance with the putative protective role of heat shock proteins. However, such elevated temperature decreased the cell expression of OVGP1 and prevented the embryos co-cultured with oviductal cells from developing beyond the four-cell stage [107].…”

Section: Heat Stressmentioning

confidence: 72%

“…In another study, long-term (168 h) exposure of bovine oviduct epithelial cells to high temperature (41 • C) increased the expression of HSP70 with no effect on cell viability [107], in accordance with the putative protective role of heat shock proteins. However, such elevated temperature decreased the cell expression of OVGP1 and prevented the embryos co-cultured with oviductal cells from developing beyond the four-cell stage [107]. Thus, the thermosensitivity of the oviduct and the protective role-or, conversely, the aggravating role-this organ could play on embryo development in the case of heat stress need further investigation.…”

Section: Heat Stressmentioning

confidence: 72%

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Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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The oviductal fluid is the first environment experienced by mammalian embryos at the very beginning of life. However, it has long been believed that the oviductal environment was not essential for proper embryonic development. Successful establishment of in vitro embryo production techniques (which completely bypass the oviduct) have reinforced this idea. Yet, it became evident that in vitro produced embryos differ markedly from their in vivo counterparts, and these differences are associated with lower pregnancy outcomes and more health issues after birth. Nowadays, researchers consider the oviduct as the most suitable microenvironment for early embryonic development and a substantial effort is made to understand its dynamic, species-specific functions. In this review, we touch on the origin and molecular components of the oviductal fluid in mammals, where recent progress has been made thanks to the wider use of mass spectrometry techniques. Some of the factors and processes known to regulate oviductal secretions, including the embryo itself, as well as ovulation, insemination, endogenous and exogenous hormones, and metabolic and heat stress, are summarized. Special emphasis is laid on farm animals because, owing to the availability of sample material and the economic importance of fertility in livestock husbandry, a large part of the work on this topic has been carried out in domestic animals used for dairy and/or meat production.

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Section: Heat Stressmentioning

confidence: 72%

Section: Heat Stressmentioning

confidence: 72%

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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“…OVGP1 is an essential factor that interacts with the zona pellucida (ZP), resulting in the formation of the barrier against polyspermy. In an in vitro experiment conducted by Rapala et al [197], the effect BOECs in the culture medium of developing embryos under heat stress conditions was investigated. They showed that HS did not affect the viability of BOECs; however, the expression of HSP70 was upregulated, while the expression of OVGP1 was significantly downregulated, negatively affecting the developmental potential of the embryos.…”

Section: Oviduct-gravid Uterusmentioning

confidence: 99%

Heat Stress: A Serious Disruptor of the Reproductive Physiology of Dairy Cows

Dovolou

Giannoulis

Nanas

et al. 2023

Animals

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Global warming is a significant threat to the sustainability and profitability of the dairy sector, not only in tropical or subtropical regions but also in temperate zones where extreme summer temperatures have become a new and challenging reality. Prolonged exposure of dairy cows to high temperatures compromises animal welfare, increases morbidity, and suppresses fertility, resulting in devastating economic losses for farmers. To counteract the deleterious effects of heat stress, cattl e employ various adaptive thermoregulatory mechanisms including molecular, endocrine, physiological, and behavioral responses. These adaptations involve the immediate secretion of heat shock proteins and cortisol, followed by a complex network of disrupted secretion of metabolic and reproductive hormones such as prolactin, ghrelin, ovarian steroid, and pituitary gonadotrophins. While the strategic heat stress mitigation measures can restore milk production through modifications of the microclimate and nutritional interventions, the summer fertility records remain at low levels compared to those of the thermoneutral periods of the year. This is because sustainment of high fertility is a multifaceted process that requires appropriate energy balance, undisrupted mode of various hormones secretion to sustain the maturation and fertilizing competence of the oocyte, the normal development of the early embryo and unhampered maternal—embryo crosstalk. In this review, we summarize the major molecular and endocrine responses to elevated temperatures in dairy cows, as well as the impacts on maturing oocytes and early embryos, and discuss the consequences that heat stress brings about in dairy cattle fertility.

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“…Another mechanism is that BOEC synthesizes growth factors, embryothropic proteins such as OVGP1, and other factors (25,29,30). In addition to synthesis of such factors, BOEC cells also express a scavenging ability to remove toxic metabolites (31). Cultured BOEC cells exhibit a variety of secretory activities; it is reported that these secretory contents may affect sperm function and early embryonic development.…”

mentioning

confidence: 99%

“…Monolayer BOEC cultures are used as coculture for in vitro preimplantation of bovine embryos (3). BOEC is also used as coculture in cattle embryos as well as in embryos of other species (31).…”

mentioning

confidence: 99%

Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

Kürüm¹,

Karahan²,

Kocamiş³

et al. 2019

Turk J Vet Anim Sci

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The oviduct creates optimal conditions for oocyte maturation, sperm capacitation, fertilization, and embryo and gamete transport (1). The different segments of the oviduct vary by means of effects on spermatozoon functions. The isthmus is the section of the oviduct which serves as a reservoir for sperm, while the ampulla is known as the fertilization area (2). The oviduct epithelial lining is composed of two kinds of cells: ciliated and secretory cells. The ciliated cells play a role in oocyte, spermatozoon, and embryo transport, while secretory cells secrete substances which are important in sperm function and early embryonal development (3). While the oviduct epithelium improves the vitality and motility of the sperm, it also fosters an environment for sperm capacitation (4). The secretions of oviduct epithelial cells vary over the cycle (2). Ovarian-derived steroids regulate oviduct physiology and reproductive events in the oviduct (5). Both estrogen and progesterone receptors are found in the bovine oviduct epithelium. Gamete maturation and breeding occur during estrogen-dominant phases of the cycle, while fertilization, early stages of clefts, and morphogenesis of Abstract: Oxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the isthmus of each sexual phase without statistical significance. The SOD activity remained steady through the cell passages in both sexual phases. CAT activity at the primary culture was higher in the ampulla compared to the isthmus in both sexual phases with a significant difference for the estral phase (P < 0.05), and it decreased over the passages with no significant differences. In conclusion, the antioxidant enzyme activity profile of BOEC did not differ by region or sexual cycle except for that of CAT, which was higher in the ampulla. Further studies should focus on SOD, GPX, and CAT activity for the mechanism of BOEC adaptation to an in vitro environment.

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Influence of elevated temperature on bovine oviduct epithelial cells (BOECs)

Cited by 8 publications

References 52 publications

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Heat Stress: A Serious Disruptor of the Reproductive Physiology of Dairy Cows

Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle

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