Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi

Osuna, Antonio; Adroher, Francisco Javier; Lupiáñez, José A.

doi:10.1016/0922-3371(90)90077-a

Cited by 36 publications

(32 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The posterior intestine, rectal ampolla, and salivary glands from wild triatomines were homogenized, and any trypanosomes present were observed with a microscope or cultured in Grace medium to facilitate detection of T. cruzi and T. rangeli (20,21). T. cruzi and T. rangeli were also detected by PCR, as previously described (7,45,46).…”

mentioning

confidence: 99%

Detection of Wolbachia Bacteria in Multiple Organs and Feces of the Triatomine Insect Rhodnius pallescens (Hemiptera, Reduviidae)

Espino

Gomez

González

et al. 2009

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

At least two types of Wolbachia bacteria were detected in wild and insectarium-raised Rhodnius pallescens, a natural vector of Trypanosoma cruzi and Trypanosoma rangeli. Wolbachia was detected in all the organs and tissues studied and in the feces, and this provided a methodological advantage for determining the presence of this endosymbiont in this host, obviating the need to kill the specimens. The occurrence of trypanosomatids in wild individuals was also studied.

show abstract

mentioning

confidence: 99%

Detection of Wolbachia Bacteria in Multiple Organs and Feces of the Triatomine Insect Rhodnius pallescens (Hemiptera, Reduviidae)

Espino

Gomez

González

et al. 2009

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…These data agree with those reported previously. 33 When the trypanosomatids were treated with Compound 2, the excretion of some of these catabolites (mainly acetate) was clearly disturbed ( Figure 4B) at the dosages assayed (IC 25 ). Similarly, succinate, L-alanine and ethanol levels rose in comparison with the control experiment.…”

Section: Resultsmentioning

confidence: 99%

“…Metacyclogenesis was induced by culturing parasites at 28 C in modified Grace's medium (Gibco) for 12 days as described previously. 25 Twelve days after cultivation at 28 C, metacyclic forms were counted to infect Vero cells. The proportion of metacyclic forms was around 40% at this stage.…”

Section: Chemicalmentioning

confidence: 99%

“…Afterward, the cells were infected in vitro with metacyclic forms of T. cruzi at a ratio of 10:1. The compounds (IC 25 concentrations, concentration required to give 25% of inhibition) were added immediately after infection and were incubated for 12 h at 37 C in a 5% CO 2 . The non-phagocytosed parasites and the drugs were removed by washing, and the infected cultures were then grown for 10 days in fresh medium without drugs.…”

Section: Chemicalmentioning

confidence: 99%

“…The parasites, at a density of 5 + 10 5 cells/mL, were cultured in their corresponding medium, containing the drugs at the IC 25 …”

mentioning

confidence: 99%

See 2 more Smart Citations

In Vitro and In Vivo Studies of the Trypanocidal Activity of Four Terpenoid Derivatives against Trypanosoma cruzi

Ramírez-Macías¹,

Marı́n²,

Chahboun³

et al. 2012

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Four terpenoid derivatives were examined for their activity against Trypanosoma cruzi. Our results show that two compounds were very active in vitro against both extra-and intracellular forms. These compounds, non-toxic for the host cells, are more effective than the reference drug benznidazole. The capacity to infect cells was negatively affected and the number of amastigotes and trypomastigotes was reduced. A wide range of ultrastructural alterations was found in the epimastigote forms treated with these compounds. Some metabolic changes occurred presumably at the level of succinate and acetate production, perhaps caused by the disturbance of the enzymes involved in sugar metabolism inside the mitochondria. In vivo results were consistent with those observed in vitro. The parasitic load was significantly lower than in the control assay with benznidazole. The effects of these products showed the reduction of the anti-T. cruzi antibodies level during the chronic stage.

show abstract

In vitro Leishmanicidal and Trypanosomicidal Properties of Imidazole‐Containing Azine and Benzoazine Derivatives

et al. 2021

View full text Add to dashboard Cite

Leishmaniasis and Chagas diseases are two of the most important parasitic diseases in the world. Both belong to the category of Neglected Tropical Diseases, and they cannot be prevented by vaccination. Their treatments are founded in outdated drugs that possess many pernicious side-effects and they're not easy to administer. With the aim of discovering new compounds that could serve as anti-trypanosomal drugs, an antiparasitic study of a synthetic compound family has been conducted. A series of new 1,4-bis(alkylamino)-and 1alkylamino-4-chloroazine and benzoazine derivatives 1-4 containing imidazole rings have been synthesized and identified.Their structures showed a possible interest based on previous work. Their in vitro anti-Leishmania infantum, anti-L. braziliensis, anti-L. donovani and anti-T. cruzi activity were tested, as well as the inhibition of Fe-SOD enzymes. It was found that some of them exhibited quite relevant values indicative of being worthy of future more detailed studies, as most of them showed activity to more than only one parasite species, especially compound 3 c was active for the three studied Leishmania species and also for T. cruzi, which is a very interesting trait as it covers a wide spectrum.

show abstract

Influence of electrolytes and non-electrolytes on growth and differentiation of Trypanosoma cruzi

Cited by 36 publications

References 11 publications

Detection of Wolbachia Bacteria in Multiple Organs and Feces of the Triatomine Insect Rhodnius pallescens (Hemiptera, Reduviidae)

Detection of Wolbachia Bacteria in Multiple Organs and Feces of the Triatomine Insect Rhodnius pallescens (Hemiptera, Reduviidae)

In Vitro and In Vivo Studies of the Trypanocidal Activity of Four Terpenoid Derivatives against Trypanosoma cruzi

In vitro Leishmanicidal and Trypanosomicidal Properties of Imidazole‐Containing Azine and Benzoazine Derivatives

Contact Info

Product

Resources

About