Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts

Abstract

In this work, an electrically conductive composite based on thermoplastic polyimide and graphene was obtained and used as a bioelectrode for electrical stimulation of human dermal fibroblasts. The values of the electrical conductivity of the obtained composite films varied from 10−15 to 102 S/m with increasing graphene content (from 0 to 5.0 wt.%). The characteristics of ionic and electronic currents flowing through the matrix with the superposition of cyclic potentials ± 100 mV were studied. The high stability of the composite was established during prolonged cycling (130 h) in an electric field with a frequency of 0.016 Hz. It was established that the composite films based on polyimide and graphene have good biocompatibility and are not toxic to fibroblast cells. It was shown that preliminary electrical stimulation increases the proliferative activity of human dermal fibroblasts in comparison with intact cells. It is revealed that an electric field with a strength E = 0.02–0.04 V/m applied to the polyimide films containing 0.5–3.0 wt.% of the graphene nanoparticles activates cellular processes (adhesion, proliferation).

Synthesis and characterization of polypyrrole composites: Optimization strategies for tailored material qualities and enhanced applications

“…The versatility of polypyrrole is further enhanced through the incorporation of various composites. Combinations with nanoparticles, carbonbased materials, or other polymers contribute to synergistic effects, offering improved mechanical strength, enhanced conductivity, and expanded functionality (Díaz-Muñoz et al, 2022; Kamalov et al, 2022;Krishna Prasad et al, 2022;Nanoparticles et al, 2023) [6,13,14,15] . The study systematically investigates the impact of different composite formulations on the overall performance of the materials, elucidating the mechanisms underlying their enhanced properties.…”

Electrically stimulated gene expression under exogenously applied electric fields

“…Electro-mechanical coupling in tissue maturation is well known for maturing cardiomyocytes from neonatal rats or from hiPSC differentiation; such maturation being facilitated by electrical or mechanical stimulation ( Godier-Furnémont et al, 2015 ; Zhang et al, 2020 ). EFs have also been shown to influence gross cellular behavior in wound healing, influencing cell proliferation ( Kamalov et al, 2022 ) and migration ( Song et al, 2004 ; Zhao et al, 2004 ; Long et al, 2018 ). Taken together, this suggests a deep connection between the voltage potentials and forces induced by EFs, ion channel signaling, and the influence these may have on cellular genetics and biochemistry.…”