Influence of eggshell matrix proteins on the precipitation of calcium carbonate (CaCO3)

Hernández-Hernández, A.; Vidal, Mary-Laure; Gómez‐Morales, Jaime; Rodríguez‐Navarro, Alejandro B.; Labas, Valérie; Gautron, Joël; Nys, Yves; García‐Ruiz, Juan Manuel

doi:10.1016/j.jcrysgro.2007.11.170

Cited by 61 publications

(58 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The same device was previously used by some authors of this paper to produce calcium carbonate crystals by the vapor diffusion sitting drop technique. [17][18][19] The above works inspired the present investigation with the aim to use the crystallization mushroom to produce carbonate substituted HA nanocrystals instead of calcium carbonate crystals. This novel method consists on the diffusion of NH 3 In order to optimize the HA crystallization, different experiments have been carried out using several calcium and phosphates salts at different concentrations by keeping the Ca/P ratio constant and equal to the HA stoichiometric value of 1.67 (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…The crystals obtained are very similar to the biological ones. [17][18][19] Both HA and CaCO 3 are known for its capability to interact with a wide variety of biomolecules like small peptides and macromolecules. [20,21] In the case of HA the study of the interaction and/or its co-crystallization with biomolecules is of great importance for several reasons: to learn more about the chemical process of the HA formation in vivo, to test the material toward the biological environment and tissues and to check the ability of HA to act as carrier for biomolecules.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Biomimetic Carbonate–Hydroxyapatite Nanocrystals Prepared by Vapor Diffusion

et al. 2010

View full text Add to dashboard Cite

Biomimetic carbonate–hydroxyapatite (HA) nanocrystals have been synthesized by using the sitting drop vapor diffusion technique, for the first time. The method consists of diffusing vapors of an aqueous solution of NH4HCO3 through drops containing an aqueous mixture of (CH3COO)2Ca and (NH4)2HPO4 in order to increase slowly their pH. This synthesis has been performed in a crystallization mushroom, a glass device developed for protein and small molecules crystallization. The concentrations of the reagents, the final pH and the crystallization time have been optimized to produce pure carbonate–HA as a single phase. X‐Ray diffraction, Fourier transformed infrared spectroscopy, and transmission electron microscopy have been utilized to characterize the synthesized carbonated substituted HA crystals which display nanometric dimensions, plate‐like morphology, and low crystallinity degree, closely resembling the inorganic phase of bones, teeth, and many pathological calcifications. This novel method may prove to be suitable for the study of the interactions and/or the co‐crystallization of hydroxyapatite with minute amounts of biomolecules, polymers, or drugs.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Biomimetic Carbonate–Hydroxyapatite Nanocrystals Prepared by Vapor Diffusion

et al. 2010

View full text Add to dashboard Cite

show abstract

“…It is abundant in eggshell membranes and is also rich in the uterine fluid at the initial stage of eggshell formation [21]. It is hence thought that lysozyme plays a regulatory role in the formation of the calcium carbonate eggshell [21][22][23][24]. In this work, we have undertaken a combined study of FTIR, XRD, and SEM to investigate the role of lysozyme on the precipitation of calcium carbonate in vitro.…”

Section: Introductionmentioning

confidence: 99%

Lysozyme mediated calcium carbonate mineralization

Wang

Sun

Xia

et al. 2009

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

“…This layer is important in its role as a barrier against microorganism penetration. In this membranes, different types of collagens (I, V, and X) have been detected (Solomon, 1991;Arias et al, 1997;Wang, Ford, Prau, & Leach, 2002;Hernández-Hernández et al, 2008). No apparent effect of thermoultrasonic treatment was observed.…”

Section: Eggshell Microstructurementioning

confidence: 70%