Influence of egg investments on in-vitro penetration of mouse eggs by misshapen spermatozoa

Krzanowska, H; Lorenc, E

doi:10.1530/jrf.0.0680057

Cited by 52 publications

(28 citation statements)

References 11 publications

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“…Abnormal sperm head shapes often arise as byproducts of aneuploidy (Prisant et al 2007;Perrin et al 2008;Revay et al 2009). In rodents, alterations of the sperm hook are correlated with decreases in fertilization success (Immler et al 2007;Firman and Simmons 2009), and severely amorphous sperm heads are inefficient at penetrating the ova (Krzanowska and Lorenc 1983;Oka et al 2007;Styrna 2008). Severity of these phenotypes also varies widely among the classical inbred strains of house mice, where the incidence of abnormal sperm is negatively correlated with fertilization success (Kawai et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

et al. 2011

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Reproductive isolation between species is often caused by deleterious interactions among loci in hybrids. Finding the genes involved in these incompatibilities provides insight into the mechanisms of speciation. With recently diverged subspecies, house mice provide a powerful system for understanding the genetics of reproductive isolation early in the speciation process. Although previous studies have yielded important clues about the genetics of hybrid male sterility in house mice, they have been restricted to F 1 sterility or incompatibilities involving the X chromosome. To provide a more complete characterization of this key reproductive barrier, we conducted an F 2 intercross between wild-derived inbred strains from two subspecies of house mice, Mus musculus musculus and Mus musculus domesticus. We identified a suite of autosomal and X-linked QTL that underlie measures of hybrid male sterility, including testis weight, sperm density, and sperm morphology. In many cases, the autosomal loci were unique to a specific sterility trait and exhibited an effect only when homozygous, underscoring the importance of examining reproductive barriers beyond the F 1 generation. We also found novel two-locus incompatibilities between the M. m. musculus X chromosome and M. m. domesticus autosomal alleles. Our results reveal a complex genetic architecture for hybrid male sterility and suggest a prominent role for reproductive barriers in advanced generations in maintaining subspecies integrity in house mice.

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Section: Discussionmentioning

confidence: 99%

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

et al. 2011

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“…The proportion varies depending upon the species or the strains (Beatty & Sharma, 1960;Beatty, 1972; Wyrobek, 1979;Krzanowska, 1981), from 4% in SW mice (Wyrobek et ai, 1975;Pogany, 1987) to 99% in the azh/azh strain (Kot & Handel, 1987). The mechanisms responsible for nuclear deformation in these cells are not understood, but deformed spermatozoa are known to penetrate the zona pellucida of oocytes in vitro (Kot & Handel, 1987) and to fertilize eggs (Moore Smith, 1970; Krzanowska & Lorenc, 1983). Deformed spermatozoa may also contribute to reproductive dysfunctions and are suspected to cause habitual abortions (Giarola & Ballerio, 1962).…”

Section: Introductionmentioning

confidence: 99%

Quantitative fluorometry of abnormal mouse sperm nuclei

Pogany¹,

Balhorn²

1992

Reproduction

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Summary. An extensive quantitative analysis of deformed mouse spermatozoa was undertaken. Improvements over previous studies included the isolation and purification of sperm nuclei, a multifaceted analytical approach using several fluorochromes and the analysis of individual nuclei classified into shape categories.Malformed sperm nuclei in BALB/c mice could not be distinguished from normal ones in terms of total and basic proteins, sulfhydryl and disulfide group concentration, DNA concentration and chromatin organization. The shape of sperm nuclei is therefore probably determined by the manner in which the internal biochemical components are assembled.

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“…Certainly more subtle changes in the sperm head may be occurring and a closer examination of head dimensions may reveal greater differences. In vitro, spermatozoa having minor changes in the head are more likely to penetrate the zona pellucida and fertilize ova than those with gross malformations (Krzanowska & Lorenc, 1983 In addition to the quality, the quantity of spermatozoa produced by aged male mice may be a factor in fertilization. The epididymal sperm concentration in aged mice which produced young was approximately twice that of aged mice failing to mate.…”

Section: Discussionmentioning

confidence: 99%

Fertilizing ability of spermatozoa from aged C57BL/6NNia mice

Parkening¹

1989

Reproduction

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Summary. Spermatozoa from C57BL/6NNia mice (7-and 25-month-old males that produced offspring and 25-month-old males incapable of producing offspring which either mated or did not mate after being paired for 1 month with proven-fertile females) were tested in in-vitro fertilization studies. The 7-month-old males fertilized the largest number of oocytes (80\p=n-\86%) in vitro and 79% of them subsequently developed into blastocysts in culture. Aged males which failed to mate fertilized the lowest number of oocytes (11\p=n-\19%) with 48% developing to blastocysts. This group of mice had the lowest number of spermatozoa in the cauda epididymidis (3\m=.\2\ m=+-\ 0\m=.\4\ m=x\105/mg tissue) with fewer motile spermatozoa (22\m=.\3\ m=+-\5\m=.\1%) than younger males. The percentage of spermatozoa retaining their acrosome after 3 h in culture was higher in aged males which had not mated when compared to younger males that had mated. After 4 h in culture, however, the number of spermatozoa that had lost their acrosome was almost identical in the two groups. Superovulated mice which were artificially inseminated with spermatozoa from 25-month-old mice that had not mated did not become pregnant. Testosterone concentrations were lowest in aged mice not mating. These concentrations may explain the poor behavioural response of these males, but whether they account for the inability of spermatozoa to fertilize ova in vitro or in vivo after artificial insemination is not known.

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Influence of egg investments on in-vitro penetration of mouse eggs by misshapen spermatozoa

Cited by 52 publications

References 11 publications

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

Quantitative fluorometry of abnormal mouse sperm nuclei

Fertilizing ability of spermatozoa from aged C57BL/6NNia mice

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