Influence of effective number of pulses on the morphological structure of teeth and bovine femur after femtosecond laser ablation

Nicolodelli, Gustavo; Lizarelli, Rosane de Fátima Zanirato; Bagnato, Vanderlei Salvador

doi:10.1117/1.jbo.17.4.048001

Cited by 18 publications

(21 citation statements)

References 30 publications

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“…No new phases such as amorphous carbon peak at 1580 and 1350 cm −1 were observed due to the laser ablation under different conditions which indicate that no carbonization occurred . Despite the laser's high fluence and low scanning speed, we observed no thermal damage in any experimental conditions, in stark contrast to previous studies . This is likely because previous studies had more overlapping pulses (the effective number of pulses per spot), increasing heat accumulation.…”

Section: Resultscontrasting

confidence: 68%

“…This is likely because previous studies had more overlapping pulses (the effective number of pulses per spot), increasing heat accumulation. In this study, there were only 94 pulses per spot, compared with 1000 and 6283 pulses per spot in Nicolodelli et al and Cangueiro's studies , respectively. These results indicate that a high fluence, when mitigated by low overlapping pulses, is less likely to cause thermal damage.…”

Section: Resultscontrasting

confidence: 62%

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Influence of environmental conditions in bovine bone ablation by ultrafast laser

Aljekhedab

Zhang

Haugen

et al. 2019

Journal of Biophotonics

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Ultrafast lasers are promising tools for surgical applications requiring precise tissue cutting. Shallow ablation depth and slow rate as well as collateral damage are common barriers limiting the use of laser in clinical applications. Localized cooling with water and/or air jet is known to reduce collateral thermal damage. We studied the influence of environmental conditions including air, compressed air flow, still water and water jet on ablation depth, ablation rate and surface morphology on bovine bone samples with an 800 nm femtosecond laser. At 15 J/cm2, no thermal effect was observed by electron microscopy and Raman spectroscopy. The experimental results indicate that environmental conditions play a significant role in laser ablation. The deepest cavity and highest ablation rate were achieved under the compressed air flow condition, which is attributed to debris removal during the ablation process. The shallowest ablation depth and lowest ablation rates were associated with water flushing. For surface morphology, smooth surface and the absence of microcracks were observed under air flow conditions, while rougher surfaces and minor microcracks were observed under other conditions. These results suggest that ultrafast ablation of bone can be more efficient and with better surface qualities if assisted with blowing air jet.

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Section: Resultscontrasting

confidence: 68%

Section: Resultscontrasting

confidence: 62%

Influence of environmental conditions in bovine bone ablation by ultrafast laser

Aljekhedab

Zhang

Haugen

et al. 2019

Journal of Biophotonics

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“…Few micrometers (< 100 µm, estimated value) could be evaluated. To achieve deeper layers, we need to polish the sample or accumulate several Laser shots [37].…”

Section: Discussionmentioning

confidence: 99%

“…LIBS (Laser Induced Breakdown Spectroscopy) is an emerging analytical technique based on atomic and ionic emission of the elemental constituents of a sample [37][38][39][40][41][42]. This technique can be recognized as a potentially useful tool for the analysis of calcified tissues such as teeth, particularly for its ability to discriminate decayed tissue from healthy tooth tissue [39][40][41].…”

Section: Introductionmentioning

confidence: 99%

After bleaching enamel remineralization using a bioactive glass-ceramic (BioSilicate®)

Rastelli¹,

Nicolodelli²,

Romano³

et al. 2016

Biomedical Glasses

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Tooth bleaching agents may weaken the tooth structure, therefore, it is important to minimize any risks of enamel and dentine damage caused by them. In this way, different materials have been used to avoid or minimize the tooth damage during bleaching. Recently, bioactive glasses have been demonstrated to be effective in mineralization of dental structures. Therefore, this study evaluated the effect of BioSilicate (a polycrystalline bioactive glass-ceramic) after bleaching by Laser-induced breakdown spectroscopy (LIBS) technique. Bovine dental blocks with 4 × 4 × 3 mm were obtained (n = 20), sequentially embedded in epoxy resin and then polished. Bleaching was performed using 35% hydrogen peroxide (Whiteness HP). Calcium (Ca) and phosphate (P) intensity values by LIBS were obtained before the treatment (T0, baselinecontrol Group), after bleaching (T1), and after BioSilicate application (T2). The use of BioSilicate after bleaching showed to be an optimal way to remineralize enamel surface making BioSilicate application a promising adjunct step to avoid or minimize the mineral loss on enamel surface after bleaching.

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“…26 Using 1700-nm fs laser pulses, subsurface ablation was demonstrated in human sclera with no damage on the surface. 27 Femtosecond laser ablation in bone has been shown to have reduced side effects using irradiation near threshold conditions, 28 accelerated bone healing, 29 and only a few cell layers of disrupted cellular membrane integrity. 25 The IntraLase fs laser has previously been shown to ablate bone (the stapes) efficiently with minimal thermal and negligible mechanical damage.…”

Section: Use Of Femtosecond Laser In Bonementioning

confidence: 99%

Preclinical investigations of articular cartilage ablation with femtosecond and pulsed infrared lasers as an alternative to microfracture surgery

Sun

Juhász

et al. 2014

J. Biomed. Opt

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Abstract. Microfracture surgery is a bone marrow stimulation technique for treating cartilage defects and injuries in the knee. Current methods rely on surgical skill and instrumentation. This study investigates the potential use of laser technology as an alternate means to create the microfracture holes. Lasers investigated in this study include an erbium:YAG laser (λ ¼ 2.94 μm), titanium:sapphire femtosecond laser system (λ ¼ 1700 nm), and Nd:glass femtosecond laser (λ ¼ 1053 nm). Bovine samples were ablated at fluences of 8 to 18 J∕cm 2 with the erbium:YAG laser, at a power of 300 AE 15 mW with the titanium:sapphire femtosecond system, and at an energy of 3 μJ∕pulse with the Nd:glass laser. Samples were digitally photographed and histological sections were taken for analysis. The erbium:YAG laser is capable of fast and efficient ablation; specimen treated with fluences of 12 and 18 J∕cm 2 experienced significant amounts of bone removal and minimal carbonization with saline hydration. The femtosecond laser systems successfully removed cartilage but not clinically significant amounts of bone. Precise tissue removal was possible but not to substantial depths due to limitations of the systems. With additional studies and development, the use of femtosecond laser systems to ablate bone may be achieved at clinically valuable ablation rates.

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Influence of effective number of pulses on the morphological structure of teeth and bovine femur after femtosecond laser ablation

Cited by 18 publications

References 30 publications

Influence of environmental conditions in bovine bone ablation by ultrafast laser

Influence of environmental conditions in bovine bone ablation by ultrafast laser

After bleaching enamel remineralization using a bioactive glass-ceramic (BioSilicate®)

Preclinical investigations of articular cartilage ablation with femtosecond and pulsed infrared lasers as an alternative to microfracture surgery

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