Influence of E‐cadherin genetic variation in canine mammary tumour risk, clinicopathological features and prognosis

Abstract

E-cadherin is a cell adhesion molecule that participates in several cellular processes that guarantee the maintenance of structural and functional integrity of epithelial tissues. E-cadherin plays an important role in mammary carcinogenesis, and various studies have demonstrated the effect of CDH1 genetic variation in risk, progression and biological behaviour of human breast cancer. Although there are some recognized genetic variations in canine CDH1 gene, their influence in canine mammary tumour development and progression has not been previously evaluated. In this study, we aim to assess the influence of CDH1 SNPs rs850805755, rs852280880 and rs852639930 in the risk, clinicopathological features and clinical outcome of canine mammary tumours. A case-control study was conducted involving 206 bitches with mammary tumours and 161 bitches free of mammary neoplasia. CDH1 SNPs rs850805755 and rs852280880 were associated with a decreased risk and a later onset of mammary tumour development. Furthermore, these SNPs were related to the development of small size carcinomas, of low histological grade and low nuclear pleomorphism. SNP rs852639930 was associated with the development of small size tumours with a non-infiltrative, noninvasive growth pattern. Data from the present investigation demonstrate that these CDH1 genetic variants could have a protective role in canine mammary tumours, by being associated with low risk of tumour development, delayed onset of the disease and less aggressive clinicopathological features. K E Y W O R D S canine mammary tumour, CDH1 gene, E-cadherin, risk, SNP

Role of Cadherins in Cancer—A Review

“…On the hand, SNP rs852639930 was characterized by a non-infiltrative, non-invasive growth pattern and development of small size tumors. Therefore, these SNP variants may indicate a low tumor development [ 113 ].…”

Establishment of stable expression of firefly luciferase and EGFP in a canine inflammatory mammary carcinoma cell line and tumor-bearing model in nude mice

“…E-cadherin is a major component of adherent junctions in normal epithelial cells and many types of carcinoma cells, where it acts as a suppressor of the motility and invasion of cells ( 38 ). N-cadherin is a mesenchymal marker in epithelial–mesenchymal transformation (EMT).…”

A Novel Canine Mammary Cancer Cell Line: Preliminary Identification and Utilization for Drug Screening Studies

“…The expression of E-cadherin is down-regulated when the intercellular connection is destroyed, which will lead to the invasion and metastasis of tumor cells, suggesting that Ecadherin expression is related to the differentiation, invasion and metastasis of malignant tumors (38). Additionally, Ecadherin has been reported to play a role in the occurrence and development of canine mammary gland tumors (39). Compared with the MDCK cell line, qRT-PCR results revealed that B-CMT and CMT7364 lacked E-cadherin expression, which has already been demonstrated in CMT7364 cells.…”