Influence of Dose, Environmental Conditions and Nuclear Volume on Survival Times in Several Gamma-irradiated Plant Species

Sparrow, A.H.; Schwemmer, Susan S.; Bottino, P.J.

doi:10.1080/09553007314551231

Cited by 5 publications

(1 citation statement)

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“…1) and no seed set was obtained using Col‐4 pollen (data not shown). In terms of reduced pollen and seed fertility, a similar ‘sensitive’ stage was identified by X‐ray irradiation (20 Gy) of rice during gametogenesis, with maximal sensitivity reported to be around meiosis (Yamakama, 1967; Sparrow et al ., 1973; Evans et al ., 1984) and at the interphase prior to meiosis (Kowyama et al ., 1994). However, this irradiation‐sensitive stage did not correspond with a high efficiency of marker locus mutations (Fig.…”

Section: Discussionmentioning

confidence: 84%

Molecular genetic analysis of pollen irradiation mutagenesis in Arabidopsis

2004

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Summary• Pollen γ -irradiation mutagenesis was systematically investigated to generate targeted mutations in Arabidopsis .• Irradiation effects on viability, germination and frequency of loci deletions were evaluated.• Mutation frequency increased with irradiation dose and varied depending upon pollen developmental stage. Meiosis was the most irradiation-sensitive stage, however, it did not equate to the highest mutation frequency. High frequencies of targeted mutations were obtained by irradiation from the second mitosis to mature pollen stages, using 400 -600 Gy. Targeted mutations could also be obtained using lower doses of γ -rays (e.g. 200 Gy) provided that pollen was irradiated at an earlier developmental stage. ms1ttg marker locus pseudo-dominants were used to verify the presence and size of the resultant deletions.• The results demonstrate that γ -irradiation of pollen is an efficient approach for generating deletions in the Arabidopsis genome. Pollen mutagenesis offers the possibility of combining single-cell selection procedures with the advantages of haploid systems, including the ability to treat large numbers of pollen grains, the absence of chimerism and direct expression of targeted alleles in the M 1 generation.

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Section: Discussionmentioning

confidence: 84%