Influence of diurnal rhythms of feeding during intestine total amylolytic activity and activity of alkaline phosphatase in juvenile fish

Кузьмина, В. В.; Strel’nikova, A. P.

doi:10.1134/s1995082908020119

Cited by 8 publications

(1 citation statement)

References 12 publications

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“…Hence, studies of digestive physiology are necessary for the development of a specific diet formulation, to determine the amount and the type of ingredients to be incorporated into the diet, which depends on the enzymatic activity present in the digestive system, particularly in the case of proteases, since protein is the most important nutrient in relation to energy and amino acid requirements in fish (Lazo et al, 2007). In fish, the digestion process involves several enzymes that are responsible for protein hydrolysis (Eroldogan et al, 2008), where the activity expressed depends on several factors such as the feeding habits, food type, among other (Kuz'mina, Strel'nikova, 2008). In this way, the characterization of digestive proteases allows the determination of the optimal conditions for enzymes to reach their maximum activity as well as stability at different temperature and pH conditions (Simpson, 2000), in addition to the effect of inhibitors on protease activity (Falcon-Hidalgo et al, 2011;Unajak et al, 2012).…”

Section: E180020[2]mentioning

confidence: 99%

Partial characterization of digestive proteases in sheepshead, Archosargus probatocephalus (Spariformes: Sparidae)

et al. 2018

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Digestive proteases were partially characterized in sheepshead juveniles, using biochemical and electrophoretic techniques. Results showed higher activity level of the stomach proteases (2.39 ± 0.02 U mg protein-1) compared to the intestinal proteases (1.6 ± 0.1 U mg protein-1). The activity of trypsin, chymotrypsin, leucine aminopeptidase and carboxypeptidase A was also recorded. The optimum temperature of the stomach proteases was recorded at 45 °C, while for intestinal proteases was recorded at 55 °C. Stomach proteases showed less stability to temperature changes than intestinal proteases. An optimum pH of 2 was recorded for stomach proteases with high stability under acidic conditions, while an optimum pH of 9 was recorded for intestinal proteases showing high stability under alkaline conditions. Stomach proteases were inhibited around 78% with Pepstatin A, indicating the presence of pepsin as the main protease. The stomach proteases zymogam revealed one active band with Rf of 0.49, this enzyme was completely inhibited by Pepstatin A. The intestinal proteases zymogram revealed four active proteases (51.3, 34.9, 27.8 and 21.2 kDa) that were inhibited by TLCK, which mainly represent a trypsin-like serine proteases. It can be conclude that digestion in sheepshead can be considered as a carnivorous species with an omnivorous tendency.

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Section: E180020[2]mentioning

confidence: 99%

Partial characterization of digestive proteases in sheepshead, Archosargus probatocephalus (Spariformes: Sparidae)

et al. 2018

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Towards the standardization of brush border purification and intestinal alkaline phosphatase quantification in fish with notes on other digestive enzymes

2018

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Assessing the activity of digestive enzymes is a common procedure in many biological, physiological and nutritional studies. After reviewing the available literature on fish digestive tract maturation and enzymatic activity (pancreatic and intestinal enzymes) published between 1994 and 2017, authors detected some possible methodological and/or interpretative inconsistencies in this kind of studies, and concluded that special attention should be paid on: i) the time of conservation of frozen samples prior their analysis, ii) the proper purification of the brush border of enterocytes by a double centrifugation step (Crane et al., 1979) when authors want to evaluate the activity of intestinal brush border enzymes in order to avoid the overestimation, particularly of alkaline phosphatase (AP), because it is present in other tissues; iii) the use of the proper reaction conditions at the normal range of values in terms of ions, temperature and intestinal alkalinity for the species of interest, and AP unit calculation. The implementation of these recommendations will promote the standardization of actual analytical procedures, as well as improve the reliability of comparative studies between different fish species or rearing procedures.

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pH values and activity of digestive enzymes in the gastrointestinal tract of fish in Lake Chany (West Siberia)

et al. 2015

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Influence of diurnal rhythms of feeding during intestine total amylolytic activity and activity of alkaline phosphatase in juvenile fish

Cited by 8 publications

References 12 publications

Partial characterization of digestive proteases in sheepshead, Archosargus probatocephalus (Spariformes: Sparidae)

Partial characterization of digestive proteases in sheepshead, Archosargus probatocephalus (Spariformes: Sparidae)

Towards the standardization of brush border purification and intestinal alkaline phosphatase quantification in fish with notes on other digestive enzymes

pH values and activity of digestive enzymes in the gastrointestinal tract of fish in Lake Chany (West Siberia)

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