Influence of Dissolved Oxygen Levels on Production of
            <scp>l</scp>
            -Asparaginase and Prodigiosin by
            <i>Serratia marcescens</i>

Heinemann, Bernard; Howard, Alma J.; Palocz, Henry J.

doi:10.1128/am.19.5.800-804.1970

Cited by 34 publications

(7 citation statements)

References 11 publications

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“…C. glutamicum LS260 is an L-lysine-producing bacterial strain that reaches a high density in the production process. Owing to the performance limitations of conventional equipment, the fermentation process often suffers from insufficient dissolved oxygen levels, which, to some extent, diminishes the advantages of the L-lysine metabolic pathway ( Heinemann et al, 1970 ). Therefore, we designed and applied a new multi-paddle-linked oxygen-enhanced bioreactor.…”

Section: Resultsmentioning

confidence: 99%

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Wang

Zhao

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: L-lysine is a bulk product. In industrial production using high-biomass fermentation, the high density of bacteria and the intensity of production require sufficient cellular respiratory metabolism for support. Conventional bioreactors often have difficulty meeting the oxygen supply conditions for this fermentation process, which is not conducive to improving the sugar-amino acid conversion rate. In this study, we designed and developed an oxygen-enhanced bioreactor to address this problem.Methods: This bioreactor optimizes the aeration mix using an internal liquid flow guide and multiple propellers.Results: Compared with a conventional bioreactor, it improved the kLa from 367.57 to 875.64 h-1, an increase of 238.22%. The results show that the oxygen supply capacity of the oxygen-enhanced bioreactor is better than that of the conventional bioreactor. Its oxygenating effect increased the dissolved oxygen in the middle and late stages of fermentation by an average of 20%. The increased viability of Corynebacterium glutamicum LS260 in the mid to late stages of growth resulted in a yield of 185.3 g/L of L-lysine, 74.57% conversion of lysine from glucose, and productivity of 2.57 g/L/h, an increase of 11.0%, 6.01%, and 8.2%, respectively, over a conventional bioreactor. Oxygen vectors can further improve the production performance of lysine strains by increasing the oxygen uptake capacity of microorganisms. We compared the effects of different oxygen vectors on the production of L-lysine from LS260 fermentation and concluded that n-dodecane was the most suitable. Bacterial growth was smoother under these conditions, with a 2.78% increase in bacterial volume, a 6.53% increase in lysine production, and a 5.83% increase in conversion. The different addition times of the oxygen vectors also affected the final yield and conversion, with the addition of oxygen vectors at 0 h, 8 h, 16 h, and 24 h of fermentation increasing the yield by 6.31%, 12.44%, 9.93%, and 7.39%, respectively, compared to fermentation without the addition of oxygen vectors. The conversion rates increased by 5.83%, 8.73%, 7.13%, and 6.13%, respectively. The best results were achieved by adding oxygen vehicles at the 8th hour of fermentation, with a lysine yield of 208.36 g/L and a conversion rate of 83.3%. In addition, n-dodecane significantly reduced the amount of foam produced during fermentation, which is beneficial for fermentation control and equipment.Conclusion: The new oxygen-enhanced bioreactor improves oxygen transfer efficiency, and oxygen vectors enhance the ability of cells to take up oxygen, which effectively solves the problem of insufficient oxygen supply during lysine fermentation. This study provides a new bioreactor and production solution for lysine fermentation.

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Section: Resultsmentioning

confidence: 99%

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Wang

Zhao

et al. 2023

Front. Bioeng. Biotechnol.

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“…Prodigiosin production in Serratia spp. is a strict oxygen-dependent process (Heinemann et al, 1970). We initially considered whether Fnr is the repressor of prodigiosin synthesis under anaerobic conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Fnr is well recognized as a global transcriptional regulator that is activated under anaerobic conditions, and prodigiosin itself is not made anaerobically (Heinemann et al, 1970). Therefore, we considered whether Fnr is the repressor responsible for inhibiting prodigiosin synthesis under anaerobic conditions.…”

Section: Fnr Represses Serratia Sp Atcc 39006 Prodigiosin Production Under Aerobic Conditionsmentioning

confidence: 99%

Fnr Negatively Regulates Prodigiosin Synthesis in Serratia sp. ATCC 39006 During Aerobic Fermentation

Sun

Zhou

et al. 2021

Front. Microbiol.

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The well-known Crp/Fnr family regulator Fnr has long been recognized as an oxygen sensor to regulate multiple biological processes, including the switch between aerobic/anaerobic metabolism, nitrogen fixation, bioluminescence, infection, and virulence. In most cases, Fnr was found to be active under anaerobic conditions. However, its role in aerobic antibiotic metabolism has not yet been revealed. In this research, we report that in the model organism, Serratia sp. ATCC 39006, Fnr (Ser39006_013370) negatively regulates prodigiosin production by binding to the spacer between the −10 and −35 region in the promoter of prodigiosin biosynthetic gene cluster under aerobic conditions. Fnr was also shown to modulate the anti-bacterial activity and motility by regulating pathway-specific regulatory genes, indicating that Fnr acts as a global regulator in Serratia sp. ATCC 39006. For the first time, we describe that Fnr regulates antibiotic synthesis in the presence of oxygen, which expands the known physiological functions of Fnr and benefits the further investigation of this important transcriptional regulator.

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“…Prodigiosin is a secondary metabolite which is produced in several species of bacteria such as Hahella chejuensis (Kim et al, 2007), Streptomyces grisiovirides (Kawasaki et al, 2009) and Serratia marcescens (Wasserman et al, 1960). Synthesis of prodigiosin is influenced by a variety of physiological and environmental factors including cell density (Haddix and Shanks, 2020), nutrient deprivation (Yin et al, 2021), physical and chemical stress (El-Bialy and Abou El-Nour, 2014), temperature (Williams et al, 1971), growth medium (Williams et al, 1971), oxygen supply (Heinemann et al, 1970), pH (Solé et al, 1994), and light (Someya et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Native Production of Prodigiosin in the Estuarine Bacterium, Vibrio gazogenes PB1, and Identification of the Associated pig Genes

et al. 2022

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Vibrio gazogenes PB1 is an estuarine bacterium that was first isolated from saltwater mud. This bacterial species possesses the metabolic capacity to produce prodigiosin which has potential uses as an anticancer agent, antibiotic, and a fungicide. We evaluated the feasibility of employing V. gazogenes PB1 as a bacterial host for the production of prodigiosin. V. gazogenes PB1 could be grown and maintained using the well-known lysogeny broth medium when supplemented with NaCl, and revived after storage at -80°C. Under batch conditions, growth of V. gazogenes PB1 in minimal media and production of prodigiosin was observed over a wide range of NaCl concentrations from 1 to 5% (w/v). The production of prodigiosin was significantly influenced by the concentration of glucose (as the carbon source), ammonium chloride (as the nitrogen source), inorganic phosphate ions, as well as pH. The greatest titer (231 mg/L) was observed in minimal media that contained 1% (w/v) glucose, 100 mM ammonium chloride and 100 mM potassium phosphate buffer. The sequences and chromosomal locations of the pig genes associated with prodigiosin biosynthesis are revealed for the first time. PigA is an isolated gene on chromosome 2, while the remaining pig genes, from pigB to pigN, exist as a 20 kb gene cluster on chromosome 1. Given its excellent growth in a range of NaCl concentrations, wide availability from culture collections and low-risk status for experimental work, we would conclude that V. gazogenes PB1 is a promising bacterial host for the production of prodigiosin.

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Influence of Dissolved Oxygen Levels on Production of l -Asparaginase and Prodigiosin by Serratia marcescens

Cited by 34 publications

References 11 publications

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Fnr Negatively Regulates Prodigiosin Synthesis in Serratia sp. ATCC 39006 During Aerobic Fermentation

Native Production of Prodigiosin in the Estuarine Bacterium, Vibrio gazogenes PB1, and Identification of the Associated pig Genes

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