Influence of disinfectants on growth of biofilm formed by <i>К. pneumoniae</i> strains

Abstract

Introduction. Currently, the problem of biofilm-associated infections is of great importance, since the classical methods of antibiotic therapy for purulent-inflammatory diseases become ineffective due to the high resistance of pathogens. An urgent task is to find ways to suppress adhesion processes or destroy an already formed biofilm using various disinfectants, enzymes, a combination of antibacterial and other drugs.Materials and methods. The object of the study is the bacteria Klebsiella pneumoniae isolated from wounds and fistulas of patients with chronic osteomyelitis. Biofilms were cultured for two days in the wells of polystyrene plates and on coverslips. In the control series of the experiment, the biofilm-forming ability of K. pneumoniae strains was studied after 48 hours. In the second series, after 24 hours, the emerging biofilm was exposed to a solution of 3 % hydrogen peroxide, in the third series, with a skin antiseptic, and after 48 hours, the intensity of biofilm formation was assessed. For statistical processing of the obtained data, the Gnumeric 1.12.17 spreadsheet program was used.Results and its discussion. K. pneumoniae strains had a high biofilm-forming ability. The impact of disinfectants on the emerging biofilm led to a decrease in the optical density in the wells of the plates and the intensity of biofilm formation. On cover slips in the second and third series of the experiment, a decrease in the total proportion of microcolonies and individual adherent cells was observed. Disinfectants (hydrogen peroxide and skin antiseptic) had a bacteriostatic effect on biofilms formed by K. pneumoniae strains. In addition, a bactericidal effect of skin antiseptic on planktonic bacterial cells was found.Conclusions. The data obtained must be taken into account for the selection of effective disinfectants used in the clinic to prevent the emergence of resistant strains of microbes.