Influence of diphenyl diselenide on chlorpyrifos-induced toxicity in Drosophila melanogaster

Adedara, Isaac A.; Klimaczewski, Cláudia Vargas; Barbosa, Nilda Vargas; Farombi, Ebenezer O.; Souza, Diogo O.

doi:10.1016/j.jtemb.2015.05.003

Cited by 29 publications

(10 citation statements)

References 49 publications

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“…Also, the inhibition of AChE is the most plausible explanation for much of the symptomatology following OP intoxication (Yamashita et al 1997). Actually, the use of AChE inhibition as biomarker to assess the toxic effects of organophosphates has been studied for a wide range of species and many different xenobiotics and is a well-accepted index of organophosphates toxicity both in vivo and in vitro (Sanchez-Hernandez and Walker 2000; Adedara et al 2015). Also, Ma et al (2014) reported that ABM exposure inhibited AChE activity in freshwater snail Physa acuta.…”

Section: Discussionmentioning

confidence: 99%

Neuro and renal toxicity induced by chlorpyrifos and abamectin in rats

Nasr

El‐Demerdash

El-Nagar

2015

Environ Sci Pollut Res

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Oxidative stress by increased production of reactive oxygen species has been implicated in pesticides toxicity. This study focused on the toxicological effects of chlorpyrifos, an organophosphate insecticide and abamectin, a biocide each alone or in combination on antioxidant status, and oxidative stress biomarkers in brain and kidney. Animals were divided into four groups. The first group was used as control while groups 2, 3, and 4 were treated with chlorpyrifos (CPF; 14.9 mg/kg BW), abamectin (ABM; 30 mg/kg BW), and chlorpyrifos plus abamectin, respectively. Rats were treated daily with the tested compounds by oral gavages for 30 days. Results revealed that thiobarbituric acid-reactive substances (TBARS) levels were significantly increased in brain and kidney due to insecticides administration. On the other hand, reduced glutathione (GSH) and protein contents in addition to the activities of antioxidant enzymes, alkaline phosphatase (ALP), and acetylcholinesterase (AChE) were significantly decreased in rat organs. A significant induction in lactate dehydrogenase (LDH) activity, urea, and creatinine levels were also observed. The response was more pronounced in rats treated with both CPF and ABM. Results showed that the used insecticides had the propensity to cause significant oxidative damage in rat brain and kidney which is associated with marked perturbations in antioxidant defense system. It can be concluded that antioxidant enzymes can be used as potential biomarkers of toxicity associated with pesticides exposure.

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Section: Discussionmentioning

confidence: 99%

Neuro and renal toxicity induced by chlorpyrifos and abamectin in rats

Nasr

El‐Demerdash

El-Nagar

2015

Environ Sci Pollut Res

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“…Drosophila is highly sensitivity to varying degrees of toxicants and is considered as a model for toxicity studies (Abolaji et al., 2013). It also serves as a useful model for evaluating biological actions of therapeutic agents against several human diseases (Adedara, Klimaczewski, 2015; Adedara, Rosemberg, 2015). Drosophila has unique physiological features similar to that of vertebrates (Baker & Thummel, 2007), and it has been certified to help reduce, refine, and replace higher animal usages such as rodents in biomedical research such as toxicity studies (Benford et al., 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary inclusions of bitter kola seed on geotactic behavior and oxidative stress markers in Drosophila melanogaster

Oboh

Ogunsuyi

Ojelade

et al. 2018

Food Science & Nutrition

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This study evaluated the effect of dietary inclusions of Garcinia kola (GK) seed on geotactic behavior and some oxidative stress markers in wildߚtype fruit flies (Drosophila melanogaster). Flies were raised on diet supplement with GK seed for 5 days. The negative geotactic behavior of flies which was used to evaluate their locomotor performance was thereafter evaluated. The flies were subsequently homogenized and the reactive oxygen species (ROS) level, acetylcholinesterase (AChE), catalase and glutathioneߚSߚtransferase (GST) activities, as well as nitric oxide (NO) and total thiol contents were assayed. The phytochemical constituents of GK seed were also determined. It was observed that higher dietary inclusions of GK seed reduced the survival rate of D. melanogaster more significantly compared to control flies. Also, higher dietary inclusions of GK seed significantly reduced locomotor performance and AChE activity, while the ROS level was increased compared to the control. Activities of GST and catalase were significantly increased in flies fed diet supplemented with higher GK seed inclusions but their NO content was significantly reduced compared to control. Phytochemical analysis of GK seed revealed abundance of saponin > glycosides > alkaloids > phenols > flavonoids. These results have shown that dietary inclusion of GK seed at higher concentrations reduced survival rate of D. melanogaster and impaired cholinergic system, with elevated activities of some antioxidant enzymes under acute exposure. These observations could be associated with bioactivities of predominant phytochemicals in GK seed such as saponin and glycosides which have been reportedly toxic at high concentration. Therefore, this study suggests that high consumption of GK seed could induce some toxicological effects and moderate consumption is hence recommended.

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“…Drosophila melanogaster is the closest invertebrate model organism to human based on the gene sequence similarity, and the high sensitivity to toxic substances favors its use as a bioindicator to evaluate the biological action of pharmacological agents [51,52]. The free clove oil dissolved in methanol showed similar efficacy in the inhibition in both cholinergic enzyme (AChE and BChE), and concentrations equal or above 10 mM were able to inhibit completely the enzymes.…”

Section: Acetylcholinesterase (Ache) and Butyrylcholinesterase (Bche)mentioning

confidence: 99%

Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes

Meneses

Marques

Leimann

et al. 2019

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Eugenol is a phenolic compound largely found in the clove essential oil that possesses promising biological activity. However, its low water solubility is a major concern and encapsulation is an alternative to improve water affinity. The objective of this work was to produce nanostructured lipid carriers (NLC) by hot homogenization/ultrasound emulsification and to evaluate the effect of free and encapsulated clove oil on the in vitro cholinesterase enzymes modulation using Drosophila melanogaster (DM) tissue. The NLC composed of a natural wax (carnauba or beeswax) and crodamol showed an average diameter between 121 and 367 nm with good dispersion and colloidal stability. The spherical shape and solid character together with the semi-crystalline environment confirm the formation of NLC. DSC analysis indicated polymorphic transition events of the waxes. In vitro tests using DM demonstrated that

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Influence of diphenyl diselenide on chlorpyrifos-induced toxicity in Drosophila melanogaster

Cited by 29 publications

References 49 publications

Neuro and renal toxicity induced by chlorpyrifos and abamectin in rats

Neuro and renal toxicity induced by chlorpyrifos and abamectin in rats

Effect of dietary inclusions of bitter kola seed on geotactic behavior and oxidative stress markers in Drosophila melanogaster

Encapsulation of clove oil in nanostructured lipid carriers from natural waxes: Preparation, characterization and in vitro evaluation of the cholinesterase enzymes

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