Influence of different types of narcosis and of neck fracture on the concentration of glycolytic intermediates and related compounds in rat liver

Seitz, Hans; Faupel, R.P.; Kampf, Sieghard C.; Tarnowski, W.

doi:10.1016/0003-9861(73)90591-2

Cited by 25 publications

(7 citation statements)

References 40 publications

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“…The effects of injury on hormones and glucose (Tables 1 and 2) were typical of those seen shortly after any severe injury in many species, including man (Gann & Lilly, 1984), except for the increase in insulin discussed below. The effects of diethyl ether anaesthesia and injection of DMSO/water were less and much more transient, although consistent with other reports (reviewed by Seitz et al, 1973) that ether anaesthesia increases corticosteroid release and sympathetic activity.…”

Section: Discussionsupporting

confidence: 90%

The effects of stress and injury on the activity of phosphoenolpyruvate carboxykinase in the liver of the rat

Rose¹,

Heath²

1986

Biochemical Journal

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The effects of stress (diethyl ether anaesthesia for 4-8 min, or intravenous injection of 0.05 ml of a dimethyl sulphoxide/water mixture) and of a scald injury given under ether anaesthesia on hepatic PEPCK (phosphoenolpyruvate carboxykinase, EC 4.1.1.32) were studied in the post-absorptive rat. Injury raised PEPCK activity by about 70% in 2 h and by over 100% in 4 h, over three times as fast as in animals that had only been handled (controls). The two stresses, both of types commonly imposed in animal experiments, had almost as much effect as injury for the first 2 h, although much less thereafter. The roles of sympathetic stimulation and corticosterone in mediating these rises were studied by using alpha beta-blockers and trilostane respectively as inhibitors. (Trilostane only decreased corticosterone concentrations to a little above control values.) The ether-induced increase was somewhat decreased by alpha beta-blockade, but was only eliminated by combined alpha beta-blockade and trilostane. After injury, however, PEPCK synthesis was unaffected by either alpha beta-blockade or trilostane, although it was decreased by their combined action; and it seems that either corticosterone or sympathetic stimulation was sufficient to stimulate PEPCK synthesis maximally. Stimulation by corticosterone was much greater than reported previously by others, for reasons that are discussed. Sympathetic stimulation may have been mediated by glucagon and cyclic AMP, since injury raised portal glucagon concentrations, and stress and injury raised those of hepatic cyclic AMP. PEPCK synthesis was, however, stimulated despite increases in portal insulin concentration, and was not related to the [insulin]/[glucagon] ratio. Thus stress and injury over-rode normal control mechanisms.

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Section: Discussionsupporting

confidence: 90%

The effects of stress and injury on the activity of phosphoenolpyruvate carboxykinase in the liver of the rat

Rose¹,

Heath²

1986

Biochemical Journal

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“…Metabolite concentrations were measured in eight different samples of milling powder, representing concentration profiles perpendicular to the surface of the freezeclamped sample between 0.2 and 2.6 mm. The control values, i.e., the "0.2-mm column," are in good agreement with what is usually found in freeze-clamped liver from pentobarbital-anaesthetized rats (6)(7)(8). In the second section (the "0.5-mm column"), ADP and AMP tend to rise, followed in the third section by a significant increase which is further augmented in deeper sections of the sample.…”

Section: The Efjkiency Of the Quenching Of Metabolic Processes In Difsupporting

confidence: 74%

Evaluation of a freeze-clamping technique designed for two- and three-dimensional metabolic studies of rat liver in vivo. Quenching efficiency and effect of clamping on tissue morphology

Quistorff

Poulsen

1980

Analytical Biochemistry

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“…The subcellular distributions of ATP and of lactate dehydrogenase (90-94 "/, cytosolic) and glutamate dehydrogenase (4 o/, cytosolic) were standard. These findings indicate that glucose 6-phosphate was essentially absent from mitochondria [44]. Thus, the glucose 6-phosphate levels determined in microdissected tissue (see Table 2) were representative for the cytosol and could be converted to cytosolic concentrations (see Table 3).…”

Section: Methodsmentioning

confidence: 80%

The Glucose/Glucose‐6‐Phosphate Cycle in the Periportal and Perivenous Zone of Rat Liver

Jungermann¹,

Heilbronn²,

Katz³

et al. 1982

European Journal of Biochemistry

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Periportal and perivenous hepatocytes contain different activities ( V ) of antagonistic key enzymes such as glucokinase and glucose-6-phosphatase. In order to get an insight into the metabolism of the periportal and perivenous area the flux rates (v) of the glucose/glucose-6-phosphate cycle were calculated on the basis of the Michaelis-Menten equation using the measured zonal concentrations of glucose and glucose 6-phosphate, the zonal activities of glucokinase and glucose-6-phosphatase previously reported and the half-saturating substrate concentrations (K,) of the two enzymes found in the literature. The concentrations of glucose were obtained as a first approximation by measuring the concentrations in portal (= periportal) and hepatovenous (= perivenous) blood; those of glucose 6-phosphate were calculated from the levels determined in microdissected periportal and perivenous liver tissue.The calculations showed (a) that the overall cycling rates agreed remarkably well with those reported for intact animals and (b) that during a normal feeding rhythm the periportal zone should catalyze net glucose output and the perivenous zone should mediate net glucose uptake, as proposed by the model of 'metabolic zonation'.Hepatocytes from the periportal (afferent) and perivenous (efferent) zone of the liver parenchyma differ in their enzyme equipments and subcellular structures [I, 21. This heterogeneity led to the model of 'metabolic zonation' [3,4]. It was proposed that the periportal hepatocytes, which are rich in glucose-6-phosphatase [5, 61, fructose-I ,6-bisphosphatase [7. 81 and phosphornolpyruvate carboxykinase [9], predominantly catalyze glucogenesis and that the perivenous cells, which are rich in glucokinase [7, 101 and pyruvate kinase [9], mainly mediate glycolysis.In the present investigation the cytosolic concentrations of glucose and of glucose-6-phosphate were measured in order to calculate the flux rates of the glucose/glucose-6-phosphate cycle in the periportal and perivenous zone of rat liver under different physiological conditions. The flux rates were to be determined using the measured zonal concentrations of glucose and glucose 6-phosphate, the zonal activities of glucokinase and glucose-6-phosphatase, as reported previously [5, 71, and the half-saturating substrate concentrations, as known from the literature [I 1 -151.It was found that the glucose concentrations in the periportal and perivenous zone were the same or formed a slight gradient depending on the metabolic situation, while the glucose-6-phosphate concentrations in the two zones were not significantly different under various conditions. Calculation of the flux rates revealed that normally the periportal zone

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Influence of different types of narcosis and of neck fracture on the concentration of glycolytic intermediates and related compounds in rat liver

Cited by 25 publications

References 40 publications

The effects of stress and injury on the activity of phosphoenolpyruvate carboxykinase in the liver of the rat

The effects of stress and injury on the activity of phosphoenolpyruvate carboxykinase in the liver of the rat

Evaluation of a freeze-clamping technique designed for two- and three-dimensional metabolic studies of rat liver in vivo. Quenching efficiency and effect of clamping on tissue morphology

The Glucose/Glucose‐6‐Phosphate Cycle in the Periportal and Perivenous Zone of Rat Liver

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