Influence of different trap solutions on the determination of root exudates in Lupinus albus L.

Valentinuzzi, Fabio; Cesco, Stefano; Tomasi, Nicola; Mimmo, Tanja

doi:10.1007/s00374-015-1015-2

Cited by 64 publications

(41 citation statements)

References 42 publications

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“…13,14) Plant growth stages as well as the nutrient deficiency such as nitrogen, phosphate, and iron have been found to significantly affect the secretion of metabolites into the rhizosphere. [15][16][17][18] In rice, for example, the amount of root exudates was the lowest at seedling stage, gradually increased until flowering, and decreased at maturity. 19) In Arabidopsis, metabolites secreted from roots were dynamically altered during development, in correlation with the transcriptomes of rhizosphere microbial communities.…”

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confidence: 98%

Developmental and nutritional regulation of isoflavone secretion from soybean roots

Sugiyama

Yamazaki

Yamashita

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Isoflavones play important roles in plant-microbe interactions in rhizospheres. Soybean roots secrete daidzein and genistein to attract rhizobia. Despite the importance of isoflavones in plant-microbe interactions, little is known about the developmental and nutritional regulation of isoflavone secretion from soybean roots. In this study, soybeans were grown in hydroponic culture, and isoflavone contents in tissues, isoflavone secretion from the roots, and the expression of isoflavone conjugates hydrolyzing beta-glucosidase (ICHG) were investigated. Isoflavone contents did not show strong growth-dependent changes, while secretion of daidzein from the roots dramatically changed, with higher secretion during vegetative stages. Coordinately, the expression of ICHG also peaked at vegetative stages. Nitrogen deficiency resulted in 8- and 15-fold increases in secretion of daidzein and genistein, respectively, with no induction of ICHG. Taken together, these results suggest that large amounts of isoflavones were secreted during vegetative stages via the hydrolysis of (malonyl)glucosides with ICHG.

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mentioning

confidence: 98%

Developmental and nutritional regulation of isoflavone secretion from soybean roots

Sugiyama

Yamazaki

Yamashita

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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“…When studying rhizosphere organic anions, the great challenge is how to extract them effectively from soil samples due to their ability to interact with soil particles (Valentinuzzi et al, 2015). Low concentrations of Ca are often added to the extractant to ensure cell integrity, limit osmotic stress, and possible leakage/diffusion.…”

Section: Discussionmentioning

confidence: 99%

“…Low concentrations of Ca are often added to the extractant to ensure cell integrity, limit osmotic stress, and possible leakage/diffusion. Although Micropur can inhibit organic anion degradation by microorganisms, it also influences the exudation and degradation processes and hence affects the results (Valentinuzzi et al, 2015). Moreover, root exudates have complicated interactions with soil microbial communities (Badri and Vivanco, 2009).…”

Section: Discussionmentioning

confidence: 99%

Rhizosphere Organic Anions Play a Minor Role in Improving Crop Species' Ability to Take Up Residual Phosphorus (P) in Agricultural Soils Low in P Availability

et al. 2016

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Many arable lands have accumulated large reserves of residual phosphorus (P) and a relatively large proportion of soil P is less available for uptake by plants. Root released organic anions are widely documented as a key physiological strategy to enhance P availability, while limited information has been generated on the contribution of rhizosphere organic anions to P utilization by crops grown in agricultural soils that are low in available P and high in extractable Ca, Al, and Fe. We studied the role of rhizosphere organic anions in P uptake from residual P in four common crops Triticum aestivum, Avena sativa, Solanum tuberosum, and Brassica napus in low- and high-P availability agricultural soils from long-term fertilization field trials in a mini-rhizotron experiment with four replications. Malate was generally the dominant organic anion. More rhizosphere citrate was detected in low P soils than in high P soil. B. napus showed 74–103% increase of malate in low P loam, compared with clay loam. A. sativa had the greatest rhizosphere citrate concentration in all soils (5.3–15.2 μmol g−1 root DW). A. sativa also showed the highest level of root colonization by arbuscular mycorrhizal fungi (AMF; 36 and 40%), the greatest root mass ratio (0.51 and 0.66) in the low-P clay loam and loam respectively, and the greatest total P uptake (5.92 mg P/mini-rhizotron) in the low-P loam. B. napus had 15–44% more rhizosphere acid phosphatase (APase) activity, ~0.1–0.4 units lower rhizosphere pH than other species, the greatest increase in rhizosphere water-soluble P in the low-P soils, and the greatest total P uptake in the low-P clay loam. Shoot P content was mainly explained by rhizosphere APase activity, water-soluble P and pH within low P soils across species. Within species, P uptake was mainly linked to rhizosphere water soluble P, APase, and pH in low P soils. The effects of rhizosphere organic anions varied among species and they appeared to play minor roles in improving P availability and uptake.

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“…The genotypic variations in tolerance to Fe deficiency in cereal species are correlated with the PS rate from roots (Marschner et al, 1986; Kawai et al, 1988). The method we adopted for PS analysis is similar to those of Valentinuzzi et al (2015) where distilled water was found to be the most suitable as trap solution for collecting root exudates from plants grown in hydroponic conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, wheat plants were removed from the hydroponic conditions 2 h after the onset of the light period and washed with deionized water for 1 min. Harvested roots were then submerged in 500 ml distilled water aerated by the hydroponic pump for 3 h. Afterward, collected exudates were passed through micropur (Roth, Germany) to prevent microbial degradation of PS (Valentinuzzi et al, 2015). After that, exudates were filtered through filter paper and concentrated to 20 ml at 50°C under vacuum.…”

Section: Methodsmentioning

confidence: 99%

Regulation of Phytosiderophore Release and Antioxidant Defense in Roots Driven by Shoot-Based Auxin Signaling Confers Tolerance to Excess Iron in Wheat

et al. 2016

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Iron (Fe) is essential but harmful for plants at toxic level. However, how wheat plants tolerate excess Fe remains vague. This study aims at elucidating the mechanisms underlying tolerance to excess Fe in wheat. Higher Fe concentration caused morpho-physiological retardation in BR 26 (sensitive) but not in BR 27 (tolerant). Phytosiderophore and 2-deoxymugineic acid showed no changes in BR 27 but significantly increased in BR 26 due to excess Fe. Further, expression of TaSAMS. TaDMAS1, and TaYSL15 significantly downregulated in BR 27 roots, while these were upregulated in BR 26 under excess Fe. It confirms that inhibition of phytosiderophore directs less Fe accumulation in BR 27. However, phytochelatin and expression of TaPCS1 and TaMT1 showed no significant induction in response to excess Fe. Furthermore, excess Fe showed increased catalase, peroxidase, and glutathione reductase activities along with glutathione, cysteine, and proline accumulation in roots in BR 27. Interestingly, BR 27 self-grafts and plants having BR 26 rootstock attached to BR 27 scion had no Fe-toxicity induced adverse effect on morphology but showed BR 27 type expressions, confirming that shoot-derived signal triggering Fe-toxicity tolerance in roots. Finally, auxin inhibitor applied with higher Fe concentration caused a significant decline in morpho-physiological parameters along with increased TaSAMS and TaDMAS1 expression in roots of BR 27, revealing the involvement of auxin signaling in response to excess Fe. These findings propose that tolerance to excess Fe in wheat is attributed to the regulation of phytosiderophore limiting Fe acquisition along with increased antioxidant defense in roots driven by shoot-derived auxin signaling.

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Influence of different trap solutions on the determination of root exudates in Lupinus albus L.

Cited by 64 publications

References 42 publications

Developmental and nutritional regulation of isoflavone secretion from soybean roots

Developmental and nutritional regulation of isoflavone secretion from soybean roots

Rhizosphere Organic Anions Play a Minor Role in Improving Crop Species' Ability to Take Up Residual Phosphorus (P) in Agricultural Soils Low in P Availability

Regulation of Phytosiderophore Release and Antioxidant Defense in Roots Driven by Shoot-Based Auxin Signaling Confers Tolerance to Excess Iron in Wheat

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