Influence of different spacer arms on Mimetic Ligand™ A2P and B14 membranes for human IgG purification

Boi, Cristiana; Dimartino, Simone; Hofer, Stefan; Horak, Jeannie; Williams, Sharon; Sarti, Giulio Cesare; Lindner, Wolfgang

doi:10.1016/j.jchromb.2011.03.059

Cited by 28 publications

(32 citation statements)

References 28 publications

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“…As already reported in previous works, the mimetic ligand B14 is highly specific towards IgG [26,29]. Therefore IgG molecules tend to adsorb strongly onto the available and accessible B14 binding sites of the membrane.…”

Section: Relevant Binding Mechanismsmentioning

confidence: 55%

“…[25] and [26]; for clarity sake the experimental set up and procedures adopted to evaluate numerical values of model parameters are briefly recalled in the following.…”

Section: Methodsmentioning

confidence: 99%

“…where c SD is the protein concentration resulting from the external system dispersion and entering the affinity membrane column, c 0 is the protein concentration in the feed tank, F is the feed flow rate, V CSTR and V PFR are the CSTR and PFR volumes, respectively; t d is the delay time associated to PFR: t d = V PFR /F; t ads 0 and t was 0 are the starting times for adsorption and washing stages, respectively [26].…”

Section: Theoretical Modelmentioning

confidence: 99%

“…[25] for the previous model; (ii) in addition, use of the bi-Langmuir kinetics allows to overcome conceptual inconsistency of the previous models where a reversible Langmuir kinetics was used during adsorption stage, while no reaction was considered during washing, even if no buffer changes were introduced in the washing stage. The experimental reference system considered for model validation is the purification of human IgG through mimetic B14-TRZ-Epoxy2 membranes, which have been produced and studied in view of the actual interest on mimetic ligands [26]. This material consists of a highly interconnected porous matrix, where mimetic ligand B14 is immobilized onto the pore surface through a triazole ring (TRZ) spacer.…”

Section: Introductionmentioning

confidence: 99%

“…These new affinity membranes combine the highly accessible internal structure of Epoxy2 membranes with the benefits of B14 ligand, represented by high specificity towards IgG and pluronic F68 tolerance. In addition, an industrial application of this chromatographic medium results particularly promising as significant improvements in its binding capacity are expected [26].…”

Section: Introductionmentioning

confidence: 99%

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Influence of protein adsorption kinetics on breakthrough broadening in membrane affinity chromatography

Dimartino¹,

Boi²,

Sarti³

2011

Journal of Chromatography A

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Section: Relevant Binding Mechanismsmentioning

confidence: 55%

“…[25] and [26]; for clarity sake the experimental set up and procedures adopted to evaluate numerical values of model parameters are briefly recalled in the following.…”

Section: Methodsmentioning

confidence: 99%

Section: Theoretical Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of protein adsorption kinetics on breakthrough broadening in membrane affinity chromatography

Dimartino¹,

Boi²,

Sarti³

2011

Journal of Chromatography A

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Porous Platform Inks for Fast and High‐Resolution 3D Printing of Stationary Phases for Downstream Processing

Conti

Symington

Pullen

et al. 2023

Adv Materials Technologies

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Purifications of biologics can be improved using 3D printed stationary phases with perfectly ordered morphology. However, limited spatial resolution and lack of porous materials have hindered application of additive manufacturing in bioprocessing. To bridge this gap, digital light processing and polymerization‐induced phase separation are combined to fabricate platform materials with bed morphology at the micrometer scale, and porous network in the nanometer scale. Four different porous inks are developed, 3D printed, and characterized in terms of their rheological behaviour, polymerization kinetics, and printing resolution. Rapid 3D printing (down to 1 h) is achieved at scale (up to 100 mL column) of porous supports (50% porosity) at high resolution (up to 50 µm for linear features and 200 µm for complex geometries). 3D‐printed gyroids are chemically functionalized with various ion exchange ligands. These are successfully challenged for i) the separation of model proteins in dynamic conditions and ii) protein capture from a clarified cell harvest, demonstrating dynamic binding capacities between 5 and 16 mg mL−1 and up to 86% purity in a single run. This work introduces a rapid and facile approach to 3D printing porous inks to fabricate perfectly ordered stationary phases for downstream processing.

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Affinity monolith chromatography: A review of general principles and recent developments

2021

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Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix. The target‐specific interaction exhibited by the binding agents makes AMC attractive for the separation or detection of a wide range of compounds. This article will review the basic principles of AMC and recent developments in this field. The supports used in AMC will be discussed, including organic, inorganic, hybrid, carbohydrate, and cryogel monoliths. Schemes for attaching binding agents to these monoliths will be examined as well, such as covalent immobilization, biospecific adsorption, entrapment, molecular imprinting, and coordination methods. An overview will then be given of binding agents that have recently been used in AMC, along with their applications. These applications will include bioaffinity chromatography, immunoaffinity chromatography, immobilized metal‐ion affinity chromatography, and dye‐ligand or biomimetic affinity chromatography. The use of AMC in chiral separations and biointeraction studies will also be discussed.

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Influence of different spacer arms on Mimetic Ligand™ A2P and B14 membranes for human IgG purification

Cited by 28 publications

References 28 publications

Influence of protein adsorption kinetics on breakthrough broadening in membrane affinity chromatography

Influence of protein adsorption kinetics on breakthrough broadening in membrane affinity chromatography

Porous Platform Inks for Fast and High‐Resolution 3D Printing of Stationary Phases for Downstream Processing

Affinity monolith chromatography: A review of general principles and recent developments

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