Influence of different semen extenders and seminal plasma on PMN migration and on expression of IL-1β, IL-6, TNF-α and COX-2 mRNA in the equine endometrium

Palm, Franziska; Walter, Ingrid; Budik, S.; Kolodziejek, Jolanta; Nowotny, Norbert; Aurich, Christine

doi:10.1016/j.theriogenology.2008.04.054

Cited by 66 publications

(49 citation statements)

References 22 publications

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“…The horse has a unique problem of inseminationinduced endometritis, which has increased with the use of SP-free AI. Interestingly, when endometrial inflammation was measured in response to SP in horse, similar increases in leukocyte infiltrate and immune mediator expression were observed when compared with infusion of sterile PBS (Palm et al 2008), suggesting mechanical stimulation of inflammation in horse, possibly due to the application of excess fluid volume in the uterus. It has been suggested that SP regulates inflammation in the equine to mediate inflammation resolution.…”

Section: Sp Signaling and Equine Reproductionmentioning

confidence: 70%

“…When sperm are resuspended into SP after cryopreservation, conception rates in the donkey were increased 166% compared with commercial extender (Rota et al 2012). However, there exist a number of studies that suggest a negative effect of SP on the cryosurvival of equine sperm at higher concentrations (above 5%) (Brinsko et al 2000, Aurich 2008. The conflicting data between improved and decreased sperm parameters may be in part due to variation of SP composition between stallions and/or preservation protocols (Aurich et al 1996).…”

Section: Sp Signaling and Equine Reproductionmentioning

confidence: 99%

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A role for seminal plasma in modulating pregnancy outcomes in domestic species

Bromfield¹

2016

Reproduction

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Seminal plasma is a complex fluid produced by the accessory glands of the male reproductive tract. Seminal plasma acts primarily as a transport medium for sperm on its arduous journey through the male and then female reproductive tract following ejaculation. This spermatozoan expedition will hopefully result in the meeting of and resultant fertilization of an oocyte, perpetuating the genetic lineage of both sexes. Whereas seminal plasma has historically been perceived as only a transport medium providing a nutrient-rich fluid environment for sperm during this exchange of genetic material, new insights into a complex communication pathway between males and females has been unraveled in the past 30 years. This new research suggests seminal plasma as a method to promote early pregnancy success by modulating cellular and molecular adaptions of the maternal environment required to facilitate healthy, successful pregnancy outcomes. Whereas much work on this exciting new communication process has focused on mice and translation to human reproduction, here we review the current evidence in domestic species where artificial insemination in the absence of seminal plasma is routine. Improving artificial insemination in domestic species to optimize offspring health and productivity could have far-reaching impacts on agriculturally relevant species such as cattle, sheep, pigs and horses.Reproduction (2016) 152 R223-R232

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Section: Sp Signaling and Equine Reproductionmentioning

confidence: 70%

Section: Sp Signaling and Equine Reproductionmentioning

confidence: 99%

A role for seminal plasma in modulating pregnancy outcomes in domestic species

Bromfield¹

2016

Reproduction

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“…SP induction of PTGS2 in the female genital tract has been demonstrated in endometrial tissues of horses and pigs and in human cervical adenocarcinoma cells [21,35,36]. More recently, SP-induced PTGS2 expression was also observed in normal cervical biopsies as part of an inflammatory-like response postulated to be necessary for promotion of fertility [16,20].…”

Section: Discussionmentioning

confidence: 97%

Seminal Plasma Induces Prostaglandin-Endoperoxide Synthase (PTGS) 2 Expression in Immortalized Human Vaginal Cells: Involvement of Semen Prostaglandin E2 in PTGS2 Upregulation1

Joseph

Zalenskaya

Sawyer

et al. 2013

Biology of Reproduction

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Inflammation of the cervicovaginal mucosa is considered a risk factor for HIV infection in heterosexual transmission. In this context, seminal plasma (SP) may play an important role that is not limited to being the main carrier for the virions. It is known that SP induces an inflammatory reaction in the cervix called postcoital leukocytic reaction, which has been associated with promotion of fertility. The mechanisms by which SP triggers this reaction, however, have not been clearly established. Previously we reported the expression of prostaglandin-endoperoxide synthase 2 (PTGS2), also known as cyclooxygenase 2 (COX-2), in human vaginal cells in response to toll-like receptor (TLR) ligands and other proinflammatory stimuli. In this study, we demonstrate that SP induces transcriptional and translational increase of COX-2 expression in human vaginal cells and cervicovaginal tissue explants. Furthermore, SP potentiates vaginal PTGS2 expression induced by other proinflammatory stimulants, such as TLR ligands and a vaginal mucosal irritant (nonoxynol-9) in a synergistic manner. SP-induced PTGS2 expression is mediated by intracellular signaling pathways involving MAPKs and NF-κB. Using fractionation and functional analysis, seminal prostaglandin (PG)-E(2) was identified as a one of the major factors in PTGS2 induction. Given the critical role of this PG-producing enzyme in mucosal inflammatory processes, the finding that SP induces and potentiates the expression of PTGS2 in cervicovaginal cells and tissues has mechanistic implications for the role of SP in fertility-associated mucosal leukocytic reaction and its potential HIV infection-enhancing effect.

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“…Studies in the pig, horse, and sheep all demonstrate the induction of endometrial cytokine expression following seminal plasma exposure with GMCSF, IL6, MCP1, and IL10 being induced in the pig (O' Leary et al 2004 ), IL1B, IL6, TNF, and cyclooxygenase 2 (COX2) in the horse (Palm et al 2008 ), and GMCSF and IL8 in the sheep (Scott et al 2009 ). Studies in the cat and camel provide evidence for a similar function for seminal fl uid as in other mammalian species (Lockett et al 2010 ;Li and Zhao 2004 ).…”

Section: Seminal Fluid and The Peri-conception Infl Ammatory Responsementioning

confidence: 99%

Seminal Fluid Signalling in the Female Reproductive Tract: Implications for Reproductive Success and Offspring Health

Schjenken

Robertson

2015

Advances in Experimental Medicine and Biology

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Carriage of sperm is not the only function of seminal fluid in mammals. Studies in mice show that at conception, seminal fluid interacts with the female reproductive tract to induce responses which influence whether or not pregnancy will occur, and to set in train effects that help shape subsequent fetal development. In particular, seminal fluid initiates female immune adaptation processes required to tolerate male transplantation antigens present in seminal fluid and inherited by the conceptus. A tolerogenic immune environment to facilitate pregnancy depends on regulatory T cells (Treg cells), which recognise male antigens and function to suppress inflammation and immune rejection responses. The female response to seminal fluid stimulates the generation of Treg cells that protect the conceptus from inflammatory damage, to support implantation and placental development. Seminal fluid also elicits molecular and cellular changes in the oviduct and endometrium that directly promote embryo development and implantation competence. The plasma fraction of seminal fluid plays a key role in this process with soluble factors, including TGFB, prostaglandin-E, and TLR4 ligands, demonstrated to contribute to the peri-conception immune environment. Recent studies show that conception in the absence of seminal plasma in mice impairs embryo development and alters fetal development to impact the phenotype of offspring, with adverse effects on adult metabolic function particularly in males. This review summarises our current understanding of the molecular responses to seminal fluid and how this contributes to the establishment of pregnancy, generation of an immune-regulatory environment and programming long-term offspring health.

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Influence of different semen extenders and seminal plasma on PMN migration and on expression of IL-1β, IL-6, TNF-α and COX-2 mRNA in the equine endometrium

Cited by 66 publications

References 22 publications

A role for seminal plasma in modulating pregnancy outcomes in domestic species

A role for seminal plasma in modulating pregnancy outcomes in domestic species

Seminal Plasma Induces Prostaglandin-Endoperoxide Synthase (PTGS) 2 Expression in Immortalized Human Vaginal Cells: Involvement of Semen Prostaglandin E2 in PTGS2 Upregulation1

Seminal Fluid Signalling in the Female Reproductive Tract: Implications for Reproductive Success and Offspring Health

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