Influence of different SAR elicitors on induction and expression of PR-proteins in Potato and Muskmelon against Oomycete pathogens

Astha,; Sekhon, P. S.; Sangha, Manjeet Kaur

doi:10.1007/s42360-018-0100-5

Cited by 11 publications

(3 citation statements)

References 15 publications

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“…The increased stimulation of defense gene processes in potato tubers treated with SA or Pro alone suggests the molecular mechanisms governing both SA and Pro to alleviate L. amnigena infection in tubers. Combined treatment of SA and Pro with L. amnigena-infected tubers induced the up-regulation of PR1, PR2, CTL1, PAL, PRH3, and SPI1, compared to the controls, which led to the most important effector genes for systemic acquired resistance (SAR) mediated by SA and Pro [96,97]. Chen et al [98] discovered that applied Pro at 0.5 mM activated PR gene expression.…”

Section: Discussionmentioning

confidence: 99%

Effects of Combined Application of Salicylic Acid and Proline on the Defense Response of Potato Tubers to Newly Emerging Soft Rot Bacteria (Lelliottia amnigena) Infection

et al. 2022

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Potato soft rot, caused by the pathogenic bacterium Lelliottia amnigena (Enterobacter amnigenus), is a serious and widespread disease affecting global potato production. Both salicylic acid (SA) and proline (Pro) play important roles in enhancing potato tuber resistance to soft rot. However, the combined effects of SA and Pro on defense responses of potato tubers to L. amnigena infection remain unknown. Hence, the combined effects of SA and Pro in controlling newly emerging potato soft rot bacteria were investigated. Sterilized healthy potato tubers were pretreated with 1.5 mM SA and 2.0 mM Pro 24 h before an inoculation of 0.3 mL of L. amnigena suspension (3.69 × 107 CFU mL−1). Rotting was noticed on the surfaces of the hole where the L. amnigena suspension was inoculated. Application of SA and Pro with L. amnigena lowered the activity of pectinase, protease, pectin lyase, and cellulase by 64.3, 77.8, 66.4 and 84.1%, and decreased malondialdehyde and hydrogen peroxide contents by 77.2% and 83.8%, respectively, compared to the control. The activities of NADPH oxidase, superoxide dismutase, peroxide, catalase, polyphenol oxidase, phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, 4-coumaryl-CoA ligase and cinnamate-4-hydroxylase were increased in the potato tubers with combined treatments by 91.4, 92.4, 91.8, 93.5, 94.9, 91.3, 96.2, 94.7 and 97.7%, respectively, compared to untreated stressed tubers. Six defense-related genes, pathogenesis-related protein, tyrosine-protein kinase, Chitinase-like protein, phenylalanine ammonia-lyase, pathogenesis-related homeodomain protein, and serine protease inhibitor, were induced in SA + Pro treatment when compared with individual application of SA or Pro. This study indicates that the combined treatment of 1.5 mM SA and 2.0 mM Pro had a synergistic effect in controlling potato soft rot caused by a newly emerging bacterium.

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Section: Discussionmentioning

confidence: 99%

Effects of Combined Application of Salicylic Acid and Proline on the Defense Response of Potato Tubers to Newly Emerging Soft Rot Bacteria (Lelliottia amnigena) Infection

et al. 2022

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“…A blanket spray of salicylic acid can be done on vegetable crops before the onset of diseases to enhance plant defense against oomycete pathogens. A blanket spray of salicylic acid (SA) was also tested against late blight potato and downy mildew of muskmelon and it gave good results (Astha et al, 2019). During this study, it was found that enzyme activity was negatively correlated with disease development ( Table 2).…”

Section: Disease Datamentioning

confidence: 95%

Biochemical basis of systemic acquired resistance induced by different Systemic Acquired Resistance (SAR) elicitors in Brassica cultivars challenge inoculated with downy mildew pathogen

Astha

Sekhon

2021

JANS

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In India, Brassica is attacked by many bacterial, fungal and viral pathogens causing various diseases among which, downy mildew caused by Hyaloperonospora brassicae, an oomycete is the most severe one. The present investigation was conducted to reduce fungicide load on Brassica by testing an alternate method of disease control. Different Systemic Acquired Resistance (SAR) compounds were tested as foliar sprays like Salicylic acid, Jasmonic acid and Bion (Benzothiadiazole-BTH) for inducing resistance in different genotypes of Raya (Brassica juncea) against downy mildew pathogen. Protein content in plants ranged between 43.5 to 57.7 mg/g fresh weight compared to 37.2 mg/g fresh weight in control. Induction of proteins and defense related enzymes was systemic in nature. The SAR compounds also surged the levels of defense related proteins, i.e. Polyphenol oxidase (PPO), Phenylalanine ammonia lyase (PAL) and pathogenesis related Pr- proteins i.e. ?-1,3 glucanase, Peroxidase (POD), from 21 to 130 per cent indicating induction of resistance. Protein profiling of treated Brassica plants was also done electrophoreticaly, which further confirmed the induction of pathogenesis-related proteins ranging from 15- 75 kDa along with some other proteins. Salicylic acid @ 500µM showed best results with 71.27 per cent disease control followed by Jasmonic acid with 69.6 per cent; whereas both, Bion and ?eta amino butyric acid gave almost 63 per cent disease control as compared to control plants. Integration of disease tolerance in Brassica varieties/genotypes combined with prophylactic spray of salicylic acid proved to be very economical for managing downy mildew disease.

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“…For example, studies have shown that when exogenous hormone MeJA is applied to bamboo root ginger, the activity of PR protein in bamboo root ginger seedlings inoculated with pathogenic fungi Ralstonia solanacearum is rapidly enhanced, which improves the disease resistance of bamboo root ginger 19 ; when cassava was infected by whiteflies, various PR genes in the body showed different changes in response to whiteflies infection, and these PR genes were largely regulated by SA and JA 20 . After the infection of potato late blight and melon downy mildew on potato and melon, exogenous application of JA and SA can induce potato and melon to produce PR protein and other proteins, which is helpful for plants to improve disease resistance 21 . SnRK2 is only a protein kinase in plants.…”

Section: Introductionmentioning

confidence: 99%

Study on signal transmission mechanism of arbuscular mycorrhizal hyphal network against root rot of Salvia miltiorrhiza

Han,

Na,

Rongchao

et al. 2023

Sci Rep

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To explore the signal transmission mechanism of the arbuscular mycorrhizal network against root rot of Salvia miltiorrhiza. In this experiment, the arbuscular mycorrhizal hyphal network was established among Salvia miltiorrhiza plants, and a two plant three-compartment culture model was established. The root of the donor Salvia miltiorrhiza was inoculated with the pathogenic fungi Fusarium solani. The changes of hormone signals such as jasmonic acid and salicylic acid and the expression of related defense genes in the recipient Salvia miltiorrhiza plants in different periods were measured, to study the underground disease resistance signal transmission mechanism among medicinal plants. Salvia miltiorrhiza can transmit the signal of resistance to root rot through the jasmonic acid pathway; When plants suffer from disease stress, the content of JA increases significantly, and the increase of JA content will inhibit the content of SA in plants; The gene expression of PR-10 gene in the roots of Salvia miltiorrhiza with arbuscular mycorrhizal network infected by pathogenic fungi was 17.56 times higher than that inoculated only with pathogenic fungi; Changes in hormone content will also cause changes in the expression of related defense genes, such as SnRK2 is inhibited by ABA in the signal transduction pathway, while JA and ABA show antagonistic changes after inoculation of pathogenic fungi in Salvia miltiorrhiza, so JA may positively regulate the expression of SnRK2 gene. Plants can transmit signals through AM hyphal network after being stressed by the pathogen Fusarium solani. In the arbuscular mycorrhizal hyphal network, JA has important significance for the signal transmission of resistance to root rot and disease resistance of Salvia miltiorrhiza, which can make Salvia miltiorrhiza ready for stress resistance and improve the stress resistance of Salvia miltiorrhiza. This experiment is of great significance to further analyze the signal transmission mechanism of the arbuscular mycorrhizal hyphal network.

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Influence of different SAR elicitors on induction and expression of PR-proteins in Potato and Muskmelon against Oomycete pathogens

Cited by 11 publications

References 15 publications

Effects of Combined Application of Salicylic Acid and Proline on the Defense Response of Potato Tubers to Newly Emerging Soft Rot Bacteria (Lelliottia amnigena) Infection

Effects of Combined Application of Salicylic Acid and Proline on the Defense Response of Potato Tubers to Newly Emerging Soft Rot Bacteria (Lelliottia amnigena) Infection

Biochemical basis of systemic acquired resistance induced by different Systemic Acquired Resistance (SAR) elicitors in Brassica cultivars challenge inoculated with downy mildew pathogen

Study on signal transmission mechanism of arbuscular mycorrhizal hyphal network against root rot of Salvia miltiorrhiza

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