Influence of Different Protein Intake on Renal Growth in Young Rats

Jakobsson, B; Celsi, Gianni; Lindblad, B. S.; Aperia, Anita

doi:10.1111/j.1651-2227.1987.tb10463.x

Cited by 27 publications

(22 citation statements)

References 18 publications

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“…Several studies have indicated that a high protein intake may influence glomerular function both in experimental animals (22) and in children with moderately reduced glomerular filtration rate (23). Our present study confirms previous findings showing a relation between the excretion of creatinine in the urine and the protein intake (24).…”

Section: Discussionsupporting

confidence: 83%

Formula with Reduced Protein Content: Effects on Growth and Protein Metabolism during Weaning

1988

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ABSTRACT. A total of 20 healthy term infants between 4 and 6 months of age were randomly assigned to either a low protein formula (F1.3) containing 1.3 g protein/100 ml or a high protein formula (F1.8) containing 1.8 g protein/ 100 ml. Both formulas were isocaloric (72 kca1/100 ml) and had a whey-casein ratio of 50:50. Ten control infants were breast-fed (BF). The mean protein intakes (including supplementary foods) were 1.9 + 0.3,2.6 + 0.2, and 1.3 f 0.2 g/kg/day, respectively. The mean concentrations of serum urea were 2.8 + 0.6 (F1.3), 4.1 + 0.6 (F1.8), and 2.2 f 0.8 mmol/liter (BF) at 6 months (F1.3 versus BF, NS, F1.8 versus BF, p < 0.001). The urine excretion of nitrogen was similar in the F1.3 and BF groups being 81 and 78 mg/kg/day. In the Fl.&group nitrogen excretion was higher, 138 mg/kg/day. Plasma concentrations of albumin, prealbumin, and transferrin were normal and similar in the groups. Weight gain was significantly higher in the F1.8 group, 22.8 + 1.7 g/kg/wk when compared to the F1.3 and BF groups, 19.9 +: 3.9 and 18.0 + 4.3 (p < 0.01), respectively. These data indicate that a decreased proteinintake from formula during weaning results in many indices of protein metabolism and growth more similar to those found in BF infants than when conventional follow-up formulas are used. (Pediatr Res 24: 297-301, 1988) Abbreviations F1.8, high protein formula F1.3, low protein formula BF, breast-fed

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Section: Discussionsupporting

confidence: 83%

Formula with Reduced Protein Content: Effects on Growth and Protein Metabolism during Weaning

1988

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“…A influência de altas cargas de eletrólitos e proteínas sobre o crescimento e a função renal foi analisada previamente na literatura 23,24 . Em ratos, dietas com 12,0, 21,0 ou 50,0% de proteína mostraram aumento do peso dos rins somente no último grupo 24 .…”

Section: Figura 1 -Evolução Das Concentrações Médias (± Desvios Padrão)unclassified

“…Em ratos, dietas com 12,0, 21,0 ou 50,0% de proteína mostraram aumento do peso dos rins somente no último grupo 24 . Os percentuais de proteína das dietas fluidas do presente estudo (7,3 ou 17,5% nos grupos que receberam fórmula de partida ou leite de vaca integral, respectivamente), como era de se esperar, não ocasionaram diferença no peso dos rins.…”

Section: Figura 1 -Evolução Das Concentrações Médias (± Desvios Padrão)unclassified

Iron absorption from infant formula and iron-fortified cow’s milk: experimental model in weanling rats

Costa

Freitas²,

Amâncio

et al. 2009

J Pediatr (Rio J)

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Objective: To compare iron absorption from infant formula and iron-fortified cow's milk.Methods: Twenty-four weanling Wistar rats were maintained in metabolic cages during the whole experiment (10 days). On the first day, the animals were divided into three similar groups according to their weight, length, hematocrit and hemoglobin levels: 1) infant formula; 2) powdered whole cow's milk fortified with iron in the same quantity and type as the formula; 3) control -powdered whole cow's milk not fortified with iron. Deionized water and diet were offered ad libitum, and the volume consumed was measured. Weight, hematocrit, and hemoglobin levels were measured on the fifth and 10th days when length, fecal occult blood, and hepatic iron levels were also analyzed.Results: Group 1 consumed less diet (450.5±26.50 mL) than group 2 (658.8±53.73 mL) and control group (532.7±19.06 mL, p < 0.001). Hemoglobin levels were higher (p < 0.001) in group 1 (12.1±1.13 g/dL) than in group 2 (9.6±1.59 g/dL) and in control group (6.2±0.97 g/dL). Hepatic iron level showed the same pattern as hemoglobin (p < 0.001). There was no difference in weight and length between the three groups (p = 0.342). There was no fecal occult blood in the any of the animals. Conclusions:Despite the lower volume consumed, the group that received formula presented higher iron absorption and hemoglobin levels than the group fed with fortified whole cow's milk. Growth was similar in the three groups. J Pediatr (Rio J). 2009;85(5):449-454:Breast milk substitutes, dietary iron, biological availability, iron deficiency. ResumoObjetivo: Comparar a absorção do ferro da fórmula de partida e do leite de vaca integral fortificado. Métodos:Foram utilizados 24 ratos machos Wistar recém-desmamados, mantidos em gaiolas metabólicas durante todo o período do experimento (10 dias). No primeiro dia, os animais foram distribuídos em três grupos semelhantes quanto ao peso, comprimento, hematócrito e hemoglobina: 1) fórmula de partida para lactentes; 2) leite de vaca integral em pó fortificado com a mesma quantidade e tipo de sal de ferro da fórmula; e 3) controle -leite de vaca integral em pó não fortificado com ferro. Água e dieta foram oferecidas ad libitum com mensuração do volume consumido. Peso, hematócrito e hemoglobina foram mensurados no quinto e no 10º dia do experimento, quando foram analisados também comprimento, sangue oculto nas fezes e teor de ferro hepático.Resultados: O grupo 1 ingeriu menor volume de dieta (450,5±26,50 mL) que os grupos 2 (658,8±53,73 mL) e controle (532,7±19,06 mL; p < 0,001). As concentrações de hemoglobina foram maiores (p < 0,001) no grupo 1 (12,1±1,13 g/dL) que nos grupos 2 (9,6±1,59 g/dL) e controle (6,2±0,97 g/dL). O teor de ferro hepático apresentou o mesmo comportamento da hemoglobina (p < 0,001). Não foi observada diferença de peso e comprimento nos três grupos (p = 0,342). Não foi detectado sangue oculto nas fezes de nenhum dos animais. Conclusões:Apesar do menor volume ingerido, o grupo que recebeu fórmula apresentou maior abso...

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“…Renal handling of urea and ammonia may be one example of this association. That these nitrogenous end products of protein metabolism regulate renal growth has been considered since the beginning of this century [1][2][3], This hypothesis largely evolved from observations that increasing dietary protein led to renal enlargement out of proportion to other organs or the total body weight of rodents [4,5], Accepted: October 25. 1994 That renal growth associated with dietary protein feed ing is the consequence of its nitrogenous metabolites is supported by recent studies evaluating renal growth regu lation by urea and ammonia in vitro.…”

Section: Introductionmentioning

confidence: 99%

Studies on Renal Growth Regulation by Urea and Ammonia in Normal Rat Kidney Cells

Logan¹

1995

Nephron

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The hypothesis that protein metabolites regulate renal growth has been considered for decades, and the overall objective of this work was to test this hypothesis in vitro using an established line of renal tubular epithelial cells, NRK-52E. The addition of urea to the medium of confluent cultures stimulated DNA synthesis which was dependent on the concentration of urea, but independent of the presence of serum. Urea enhanced proliferation of subcon-fiuent cultures of NRK cells in the presence of dilute serum to a similar degree as epidermal growth factor (EGF). Either deletion of serum from the medium or allowing the cultures to achieve confluence prevented the proliferative response to urea, but not EGF. Ammonium chloride stimulated the uptake of ³H-thymidine but did not increase cell number. Ammonium chloride increased the uptake of ³H-leucine and total cellular protein/DNA, while urea had no effect on these markers of growth. The rate of hydrolysis of urea to ammonia in vitro was not altered by the presence of NRK cells. These results suggest that urea and ammonia may regulate renal mass, and that their actions are separate and different.

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Influence of Different Protein Intake on Renal Growth in Young Rats

Cited by 27 publications

References 18 publications

Formula with Reduced Protein Content: Effects on Growth and Protein Metabolism during Weaning

Formula with Reduced Protein Content: Effects on Growth and Protein Metabolism during Weaning

Iron absorption from infant formula and iron-fortified cow’s milk: experimental model in weanling rats

Studies on Renal Growth Regulation by Urea and Ammonia in Normal Rat Kidney Cells

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