Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in <em>Bacopa monnieri</em>(L.)

Abstract

Abstract:Bacopa monnieri (L.) Wettst. (Plantaginaceae), commonly known as Brahmi, is used in Ayurveda as a medicinal herb, found throughout the Indian subcontinent in wet, damp and marshy areas. The herb yields pharmaceutically active compounds called bacosides. The current investigation was carried out to assess the potential for increasing biomass and the concentration of bacoside A from the in vitro regenerated shoots in different media (MS, B5, NN and N6), the strength of the MS medium (0.25, 0.50, 0.75, 1.0, 1.50 and 2.0X) and carbon source [sucrose, glucose, fructose, maltose, sucrose + glucose (1:1), sucrose + maltose (1:1), glucose + fructose (1:1), glucose + maltose (1:1), fructose + sucrose (1:1) and fructose + maltose (1:1)]. All the culture media were supplemented with 2 mg/L Kinetin (Kin) at pH 5.8. High performance liquid chromatography (HPLC) was used to determine the bacoside A content from the regenerated shoots. Optimum number of adventitious shoots (70.75 shoots per explant), fresh weight (2.344 g), dry weight (0.166 g) and bacoside A content (13.052 mg/g DW) were obtained from the cultures grown in the MS medium. Bacoside A production was highest in the shoots grown in the glucose + fructose combination (15.588 mg/g DW) among the different carbon sources tested. These findings suggest that B. monnieri culture requires full strength MS medium and a moderate level of sucrose. The present study is useful to obtain optimum production of biomass and bacoside A from the in vitro regenerated shoots of B. monnieri.

In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

“…Based on the growth vigor and morphological changes, the full-strength basal medium produced more vigorous shoots and greener leaves compared to half-strength basal medium which produced yellowish leaves. The full-strength basal medium was also found to be most effective for the micropropagation of Ocimum basilicum [ 24 ], Bacopa monnieri [ 25 ] and Thymus hyemalis [ 26 ]. All of these findings were in agreement with current findings, indicating the effectiveness of the full-strength MS basal medium for the medicinal plant studied.…”

“…The addition of 30 g/L sucrose was found to be the optimum for the growth of Thymus hyemalis compared to other types of carbon sources including glucose, fructose, mannitol and sorbitol [ 17 ]. The studies implied that a sucrose concentration higher than 30 g/L results in a decrease in the multiplication and growth rate of plants [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. These findings were in agreement with the present study in which 30 g/L of sucrose was established as the optimum for the growth of C. nutans .…”

Influence of Different Concentrations of Murashige and Skoog Medium on Multiple Shoot Regeneration of Staurogyne repens (Nees) Kuntze

“…Different levels of MS nutrient salts affected the shoot regeneration values of the plant. Similarly, it has been reported that different MS nutrient salts affect the growth-development or regeneration values of plants such as Mentha spicata L. [14], Bacopa monnieri (L.) [15] and Lophophora williamsii (Lem.) Coult.…”

In vitro acclimatization of <i>Curcuma longa</i> under controlled <i>iso</i>-osmotic conditions

“…In cassumunar ginger (Zingiber montanum), phenolic, flavonoid, tannin, alkaloid, saponin and steroid in induced micro-rhizomes using 6% sucrose were enriched over the natural rhizomes, whereas curcuminoid content was equal with natural rhizomes (Rajkumari and Sanatombi 2020). In Brahmi (Bacopa monnieri), mixed sucrose/maltose or glucose/ fructose was recommended for bacoside A production in the leaf tissues (Naik et al 2017). It is possible that total curcuminoids in mini-rhizome of acclimatized turmeric plants under iso-osmotic conditions may regulate in the long-term cultivation in ex vitro conditions.…”