Influence of Different Lignocellulose Sources on Endo-1,4-β-Glucanase Gene Expression and Enzymatic Activity of Bacillus amyloliquefaciens B31C

Pasqua, Rosangela Di; Ventorino, Valeria; Aliberti, A; Robertiello, Alessandro; Faraco, Vincenza; Viscardi, Sharon; Pepe, Olimpia

doi:10.15376/biores.9.1.1303-1310

Cited by 18 publications

(14 citation statements)

References 7 publications

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“…According to our results, the strain B. amyloliquefaciens isolated from date was promising for the production of ethanol. These results are in agreement with those of Di Pasqua et al (2014) who showed a high enzymatic activity on lignocellulosic biomass by B. amyloliquefaciens isolated from compost. A microbial mixing gave better ethanol productivity than pure culture of a given strain (Kalyani et al 2013).…”

Section: Kinetics Of Ethanol Production By Bacillus Amyloliquefacienssupporting

confidence: 93%

Identification of strain isolated from dates (Phœnix dactylifera L.) for enhancing very high gravity ethanol production

Djelal¹,

Chniti²,

Jemni³

et al. 2016

Environ Sci Pollut Res

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Ethanol production from by-products of dates in very high gravity was conducted in batch fermentation using two yeasts, Saccharomyces cerevisiae and Zygosaccharomyces rouxii, as well as a native strain: an osmophilic strain of bacteria which was isolated for the first time from the juice of dates (Phoenix dactylifera L.). The phylogenetic analysis based on the 16S ribosomal RNA and gyrB sequence and physiological analysis indicated that the strain identified belongs to the genus of Bacillus, B. amyloliquefaciens. The ethanol yields produced from the syrup of dates (175 g L and 360 g L of total sugar) were 40.6% and 29.5%, respectively. By comparing the ethanol production by the isolated bacteria to that obtained using Z. rouxii and S. cerevisiae, it can be concluded that B. amyloliquefaciens was suitable for ethanol production from the syrup of dates and can consume the three types of sugar (glucose, fructose, and sucrose). Using Z. rouxii, fructose was preferentially consumed, while glucose was consumed only after fructose depletion. From this, B. amyloliquefaciens was promising for the bioethanol industry. In addition, this latter showed a good tolerance for high sugar concentration (36%), allowing ethanol production in batch fermentation at pH 5.0 and 28 °C in date syrup medium. Promising ethanol yield produced to sugar consumed were observed for the two osmotolerant microorganisms, Z. rouxii and B. amyloliquefaciens, nearly 32-33%, which were further improved when they were cocultivated, leading to an ethanol to glucose yield of 42-43%.

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Section: Kinetics Of Ethanol Production By Bacillus Amyloliquefacienssupporting

confidence: 93%

Identification of strain isolated from dates (Phœnix dactylifera L.) for enhancing very high gravity ethanol production

Djelal¹,

Chniti²,

Jemni³

et al. 2016

Environ Sci Pollut Res

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“…In the open field experiment, Bacilli increased in both the A. donax and P. nigra biomasses. Members belonging to this taxon are known to have specific genes encoding enzymes involved in cellulose and hemicellulose degradation 24 25 .…”

Section: Discussionmentioning

confidence: 99%

Exploring the microbiota dynamics related to vegetable biomasses degradation and study of lignocellulose-degrading bacteria for industrial biotechnological application

Ventorino

Aliberti

Faraco

et al. 2015

Sci Rep

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104

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The aims of this study were to evaluate the microbial diversity of different lignocellulosic biomasses during degradation under natural conditions and to isolate, select, characterise new well-adapted bacterial strains to detect potentially improved enzyme-producing bacteria. The microbiota of biomass piles of Arundo donax, Eucalyptus camaldulensis and Populus nigra were evaluated by high-throughput sequencing. A highly complex bacterial community was found, composed of ubiquitous bacteria, with the highest representation by the Actinobacteria, Proteobacteria, Bacteroidetes and Firmicutes phyla. The abundances of the major and minor taxa retrieved during the process were determined by the selective pressure produced by the lignocellulosic plant species and degradation conditions. Moreover, cellulolytic bacteria were isolated using differential substrates and screened for cellulase, cellobiase, xylanase, pectinase and ligninase activities. Forty strains that showed multienzymatic activity were selected and identified. The highest endo-cellulase activity was seen in Promicromonospora sukumoe CE86 and Isoptericola variabilis CA84, which were able to degrade cellulose, cellobiose and xylan. Sixty-two percent of bacterial strains tested exhibited high extracellular endo-1,4-ß-glucanase activity in liquid media. These approaches show that the microbiota of lignocellulosic biomasses can be considered an important source of bacterial strains to upgrade the feasibility of lignocellulose conversion for the ‘greener' technology of second-generation biofuels.

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“…Agri-food wastes are additional cheap, sustainable and attractive substrate for the production of biopolymers or other high value-added products [21]. Many studies have assessed the recycling and the potential use of agri-food wastes and byproducts or dedicated energy crops for producing polyhydroxyalkanoate (PHA) [22,23], succinic acid [24][25][26][27], biofuels and biogas [19,[28][29][30][31][32] as well as biological hydrogen and volatile fatty acids [33,34]. Natural environments represent important sources of microbial strains that exert interesting enzymatic activities for biotechnological applications [35].…”

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confidence: 99%

Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse

Ventorino

Nicolaus

Donato

et al. 2019

Chem. Biol. Technol. Agric.

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Background: Exopolysaccharides (EPSs) belong to a family of organic thickeners or alternative hydrocolloids of microbial origin. Because the chemical structure offers beneficial bioactive functions, biocompatibility and biodegradability, EPSs are used in the chemical, food, pharmaceutical, cosmetics, and packaging industries as well as in agriculture and medicine. In this study, new bacterial strains were selected on the basis of their ability to synthesize EPS from substrate containing vinasse as a nutrient source to identify the best candidate for bio-based polymer production. Results: Among the 99 newly identified bacterial strains isolated from different natural ecosystem, the strain Azotobacter chroococcum 76A was selected as the best biopolymer producer since it synthesized the highest concentration of EPS in all media containing vinasse. The maximum EPS concentration (44.6 ± 0.63 mg/50 mL) was observed at 24 h, corresponding to its sub-stationary growth phase (7 × 10 8 ± 0.29 CFU/mL). Chemical characterization of the EPS produced showed that carbohydrates representing the principal component, followed by uronic acids and proteins. Interestingly, comparing the IR spectrum of the EPS with alginate by FTIR-ATR analysis revealed an overlap of a peak identified as guluronic acid, a component of alginate. Conclusions: The potential biotechnological capacity of A. chroococcum 76A to synthetize biopolymer from vinasse, inexpensive starting materials, represents a possible alternative to expensive disposal of agri-food waste through its transformation into high value-added products.

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Influence of Different Lignocellulose Sources on Endo-1,4-β-Glucanase Gene Expression and Enzymatic Activity of Bacillus amyloliquefaciens B31C

Cited by 18 publications

References 7 publications

Identification of strain isolated from dates (Phœnix dactylifera L.) for enhancing very high gravity ethanol production

Identification of strain isolated from dates (Phœnix dactylifera L.) for enhancing very high gravity ethanol production

Exploring the microbiota dynamics related to vegetable biomasses degradation and study of lignocellulose-degrading bacteria for industrial biotechnological application

Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse

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