Influence of different growth factors on chondrogenic differentiation of adipose-derived stem cells in polyurethane-fibrin composites

Froelich, Katrin; Setiawan, Lydia E.; Technau, Antje; Tirado, Mario Ramos; Hackenberg, Stephan; Hagen, Rudolf; Staudenmaier, R.; Kleinsasser, Norbert

doi:10.5301/ijao.5000132

Cited by 7 publications

(9 citation statements)

References 62 publications

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“…of studies (%)ReferencesGrowth factorNo. of studies (%)ReferencesTGF-β148 (44%)[50, 169–175, 189, 190, 192, 193, 195, 199, 202, 208, 210, 211, 213, 214, 216, 217, 220, 222–224, 228, 230–232, 234, 235, 244, 246, 249, 252–256, 258, 260–263, 266, 267, 270]SOX-51 (1%)[204]TGF-β332 (29%)[51, 162, 164, 168, 177, 181–184, 197, 200, 205–207, 218, 223–225, 227, 237, 239, 240, 245, 247, 248, 250, 251, 257, 259, 267, 268, 270]SOX-61 (1%)[204]BMP-213 (12%)[188, 202, 213, 219, 225–227, 229, 264, 265, 267, 270, 271]WNT3A1 (1%)…”

Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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BackgroundThe management of articular cartilage defects presents many clinical challenges due to its avascular, aneural and alymphatic nature. Bone marrow stimulation techniques, such as microfracture, are the most frequently used method in clinical practice however the resulting mixed fibrocartilage tissue which is inferior to native hyaline cartilage. Other methods have shown promise but are far from perfect. There is an unmet need and growing interest in regenerative medicine and tissue engineering to improve the outcome for patients requiring cartilage repair. Many published reviews on cartilage repair only list human clinical trials, underestimating the wealth of basic sciences and animal studies that are precursors to future research. We therefore set out to perform a systematic review of the literature to assess the translation of stem cell therapy to explore what research had been carried out at each of the stages of translation from bench-top (in vitro), animal (pre-clinical) and human studies (clinical) and assemble an evidence-based cascade for the responsible introduction of stem cell therapy for cartilage defects.Main body of abstractThis review was conducted in accordance to PRISMA guidelines using CINHAL, MEDLINE, EMBASE, Scopus and Web of Knowledge databases from 1st January 1900 to 30th June 2015. In total, there were 2880 studies identified of which 252 studies were included for analysis (100 articles for in vitro studies, 111 studies for animal studies; and 31 studies for human studies). There was a huge variance in cell source in pre-clinical studies both of terms of animal used, location of harvest (fat, marrow, blood or synovium) and allogeneicity. The use of scaffolds, growth factors, number of cell passages and number of cells used was hugely heterogeneous.Short conclusionsThis review offers a comprehensive assessment of the evidence behind the translation of basic science to the clinical practice of cartilage repair. It has revealed a lack of connectivity between the in vitro, pre-clinical and human data and a patchwork quilt of synergistic evidence. Drivers for progress in this space are largely driven by patient demand, surgeon inquisition and a regulatory framework that is learning at the same pace as new developments take place.

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Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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“…Various synthetic polymers have also been extensively utilized for cartilage tissue engineering, which includes poly (glycolic acid) (PGA), poly (lactic acid) (PLA), and poly (lactic‐co‐glycolic acid) (PLGA) copolymers, poly ( L ‐lactic‐co‐glycolic acid) (PLLGA), PEG (polyethylene glycol), polyurethanes etc . These polymers have been approved by FDA for use in the form of biodegradable sutures for more than 20 years.…”

Section: Polymeric Matrices Used For Cartilage Tissue Engineeringmentioning

confidence: 99%

Tissue-Engineered Cartilage: The Crossroads of Biomaterials, Cells and Stimulating Factors

2014

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Damage to cartilage represents one of the most challenging tasks of musculoskeletal therapeutics due to its limited propensity for healing and regenerative capabilities. Lack of current treatments to restore cartilage tissue function has prompted research in this rapidly emerging field of tissue regeneration of functional cartilage tissue substitutes. The development of cartilaginous tissue largely depends on the combination of appropriate biomaterials, cell source, and stimulating factors. Over the years, various biomaterials have been utilized for cartilage repair, but outcomes are far from achieving native cartilage architecture and function. This highlights the need for exploration of suitable biomaterials and stimulating factors for cartilage regeneration. With these perspectives, we aim to present an overview of cartilage tissue engineering with recent progress, development, and major steps taken toward the generation of functional cartilage tissue. In this review, we have discussed the advances and problems in tissue engineering of cartilage with strong emphasis on the utilization of natural polymeric biomaterials, various cell sources, and stimulating factors such as biophysical stimuli, mechanical stimuli, dynamic culture, and growth factors used so far in cartilage regeneration. Finally, we have focused on clinical trials, recent innovations, and future prospects related to cartilage engineering.

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“…Though some of these initial studies on BMSCs chronogenesis aided by either TGF-β3 or BMP-2 have been promising, more wide-spread clinical utility of these approaches is limited by the short half-lives [16] of the proteins as well as prohibitive capital investment requirements [17] . To overcome these obstacles, in the present study we sought to used the adenovirus-mediated bone morphogenetic protein 2 (Ad-BMP-2) and transforming growth factor beta 3 (Ad-TGF-β3) that were constructed to infect BMSCs to construct a new type of tissue engineering scaffold in a Demineralized Bone Matrix(DBM).…”

Section: Introductionmentioning

confidence: 99%

Demineralized Bone Matrix Combined Bone Marrow Mesenchymal Stem Cells, Bone Morphogenetic Protein-2 and Transforming Growth Factor-β3 Gene Promoted Pig Cartilage Defect Repair

Wang

Han

et al. 2014

PLoS ONE

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ObjectivesTo investigate whether a combination of demineralized bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) infected with adenovirus-mediated- bone morphogenetic protein (Ad-BMP-2) and transforming growth factor-β3 (Ad-TGF-β3) promotes the repair of the full-thickness cartilage lesions in pig model.MethodsBMSCs isolated from pig were cultured and infected with Ad-BMP-2(B group), Ad-TGF-β3 (T group), Ad-BMP-2 + Ad-TGF-β3(BT group), cells infected with empty Ad served as a negative group(N group), the expression of the BMP-2 and TGF-β3 were confirmed by immunofluorescence, PCR, and ELISA, the expression of SOX-9, type II collagen(COL-2A), aggrecan (ACAN) in each group were evaluated by real-time PCR at 1w, 2w, 3w, respectively. The chondrogenic differentiation of BMSCs was evaluated by type II collagen at 21d with immunohistochemical staining. The third-passage BMSCs infected with Ad-BMP-2 and Ad-TGF-β3 were suspended and cultured with DBM for 6 days to construct a new type of tissue engineering scaffold to repair full-thickness cartilage lesions in the femur condyles of pig knee, the regenerated tissue was evaluated at 1,2 and 3 months after surgery by gross appearance, H&E, safranin O staining and O'driscoll score.ResultsAd-BMP-2 and Ad-TGF-β3 (BT group) infected cells acquired strong type II collagen staining compared with Ad-BMP-2 (B group) and Ad-TGF-β3 (T group) along. The Ad-BMP-2 and Ad-TGF-β3 infected BMSCs adhered and propagated well in DBM and the new type of tissue engineering scaffold produced hyaline cartilage morphology containing a stronger type II collagen and safranin O staining, the O'driscoll score was higher than other groups.ConclusionsThe DBM compound with Ad-BMP-2 and Ad-TGF-β3 infected BMSCs scaffold has a good biocompatibility and could well induce cartilage regeneration to repair the defects of joint cartilage. This technology may be efficiently employed for cartilage lesions repair in vivo.

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Influence of different growth factors on chondrogenic differentiation of adipose-derived stem cells in polyurethane-fibrin composites

Cited by 7 publications

References 62 publications

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

Tissue-Engineered Cartilage: The Crossroads of Biomaterials, Cells and Stimulating Factors

Demineralized Bone Matrix Combined Bone Marrow Mesenchymal Stem Cells, Bone Morphogenetic Protein-2 and Transforming Growth Factor-β3 Gene Promoted Pig Cartilage Defect Repair

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