2000
DOI: 10.1046/j.1365-2990.2000.00241.x
|View full text |Cite
|
Sign up to set email alerts
|

Influence of different fixation procedures on the quantification of infarction and oedema in a rat model of stroke

Abstract: In pharmacodynamic studies using focal ischaemia models, the size of the infarct measured by quantitative histology is the most important outcome measure. Precise, unbiased and reproducible assessment of infarct volume is of foremost importance. A frequent problem in interventional stroke models is the evaluation of infarcts in animals found dead, where instant post-mortem fixation of the brain cannot be performed. The purpose of this study was to investigate possible bias from perfusion, immediate and 3-h pos… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

1
9
0

Year Published

2003
2003
2021
2021

Publication Types

Select...
5
3

Relationship

0
8

Authors

Journals

citations
Cited by 19 publications
(10 citation statements)
references
References 30 publications
1
9
0
Order By: Relevance
“…The 3 different fixation procedures were evenly distributed without significant differences between the 3 groups with different grades of vascular occlusion. As shown previously, fixation procedures had no influence on the results of this current investigation, because infarct and oedema volumes calculated as percentages of the corresponding hemisphere were independent of fixation procedures [6]. …”
Section: Methodsmentioning
confidence: 56%
See 2 more Smart Citations
“…The 3 different fixation procedures were evenly distributed without significant differences between the 3 groups with different grades of vascular occlusion. As shown previously, fixation procedures had no influence on the results of this current investigation, because infarct and oedema volumes calculated as percentages of the corresponding hemisphere were independent of fixation procedures [6]. …”
Section: Methodsmentioning
confidence: 56%
“…Animals can die from herniation caused by large hemispheric infarcts and ipsilateral brain oedema within 24-48 hours [29]. As shown previously the three different fixation procedures did not interfere with the measurement of oedema, which after 24 hours constituted 11% of the infarct volume [6], compared to 6% using comparable methods [28], provided all enlargement of the right hemisphere was located inside the infarction, which may not be absolutely valid [30]. The calculations reported by Leach et al [31] and Swanson et al [32] compensate for these problems, and the volume effect of oedema is attempted weighted out.…”
Section: Discussionmentioning
confidence: 97%
See 1 more Smart Citation
“…However, the disadvantage of histology is that only a small portion of a given region is sampled, whereas imaging allows the signal to be averaged across the entire region of issue. Histology also requires fixation which, depending on the precise procedure used, can cause shrinkage and dehydration, potentially distorting volume measurements (Overgaard and Meden, 2000). …”
Section: Discussionmentioning
confidence: 99%
“…This presents a problem however, as the histological preparation of neural tissue is known to introduce a number of morphological anomalies. Tissue perfusion, removal, staining, and dehydration can lead to artificial tissue shrinkage, nicks in surface brain areas, and changes in tissue shape and location (Garman, 1990;Overgaard and Meden, 2000;Sadowski et al, 1995;Simmons and Swanson, 2009;Skoglund et al, 1996). It is possible that any cortical thinning, movement, volume, or density loss observed after stroke in the adult rat could be exaggerated by histological preparation.…”
Section: Introductionmentioning
confidence: 99%