Influence of different fermentable carbohydrates on dual-species biofilms of S. mutans and A. naeslundii – a pilot study

Oliveira, Rosa Virginia Dutra de; Albuquerque, Yasmin Etienne; Spolidório, Denise Madalena Palomari; Koga-Ito, Cristiane Yumi; Brighenti, Fernanda Lourenção

doi:10.14295/bds.2015.v18i2.1100

Cited by 2 publications

(1 citation statement)

References 25 publications

(46 reference statements)

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“…The presence of glucose may assist S. mutans in producing karyogenic biofilms. 9 10 11 A study 12 reported that adding lactose to the growth medium significantly stimulated the formation of biofilms from S. mutans .…”

Section: Introductionmentioning

confidence: 99%

Examination of the Structure and Formation Streptococcus mutans Biofilm Induced by Glucose, Lactose, Soy Protein, and Iron

Kriswandini,

Budi,

Justitia

2024

Eur J Dent

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Objective Streptococcus mutans, the main causative agent of caries, have the ability to form biofilms on the surface of teeth. The availability of nutrients such as glucose, lactose, soy protein, and iron can influence S. mutans in biofilm formation. All four sources of nutrients have been shown to increase the formation of S. mutans biofilms. The purpose of this study was to determine the structure and thickness of S. mutans biofilms induced by glucose, lactose, soy protein, and iron. Materials and Methods This experimental laboratory study aimed to examine the formation of biofilm structures (chemical elements) and determine the thickness of S. mutans biofilms induced by glucose, lactose, soy protein, and iron. The structures (chemical elements) were examined using scanning electron microscopy-energy-dispersive X-ray (SEM-EDX) analysis. Confocal laser scanning microscopy (CLSM) was used to determine the thickness of S. mutans biofilms with an Olympus FV1000 microscope, and the findings were analyzed using Olympus Fluoview Ver. 4.2a software. Results It was established that the results of SEM-EDX examination of the structure of S. mutans biofilms induced by glucose had oxygen (O) as the dominant chemical element (30.24 w%); lactose reported oxygen (O) as the dominant element (29.65 w%); soy protein had carbon (C) as the dominant element (34.31 w%); and iron showed oxygen (O) as the dominant element (32.51 w%). The thickness (measured by the CLSM examination) of biofilms induced by glucose, lactose, soy protein, and iron were 17,666, 12,666, 18,000, and 15,666 nm, respectively. Conclusion The structure of S. mutans biofilms induced by glucose, lactose, and iron contain the following elements in amounts from the highest to lowest: O, C, N, P, and S; the biofilm produced by S. mutans induced by soy protein in amounts from the highest to lowest comprised the elements: C, O, N, S, and P. The S. mutans biofilms induced by soy protein had the maximum thickness, followed by those induced by glucose, iron, and lactose.

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Section: Introductionmentioning

confidence: 99%

Examination of the Structure and Formation Streptococcus mutans Biofilm Induced by Glucose, Lactose, Soy Protein, and Iron

Kriswandini,

Budi,

Justitia

2024

Eur J Dent

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Effects of Sucrose and Farnesol on Biofilm Formation by Streptococcus mutans and Candida albicans

Wint,

Miyanohara,

Terada-Ito

et al. 2024

Microorganisms

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Candida albicans (C. albicans) and Streptococcus mutans (S. mutans) are frequently detected in the plaque biofilms of children with early childhood caries. This study investigated the effects of sucrose and farnesol on biofilm formation by the oral pathogens S. mutans and C. albicans, including their synergistic interactions. Biofilm formation dynamics were monitored using the Cell Index (CI). The CI for S. mutans increased in the brain–heart infusion medium, peaking at 10 h; however, the addition of sucrose reduced the CI. For C. albicans yeast cells, the CI increased at sucrose concentrations > 0.5%, peaking at 2 h. Mixed cultures of S. mutans and C. albicans yeast cells showed significantly higher CI values in the presence of sucrose, suggesting a synergistic effect on biofilm formation. Farnesol consistently suppressed biofilm formation by C. albicans yeast cells, even in the presence of sucrose, and higher farnesol concentrations resulted in greater inhibition. Regarding C. albicans hyphal cells, sucrose did not enhance biofilm formation, whereas farnesol significantly reduced biofilm formation at all concentrations tested. These findings elucidate the complex roles of sucrose and farnesol in biofilm formation by S. mutans and C. albicans and emphasize the potential of farnesol as an effective oral biofilm inhibitor.

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Influence of different fermentable carbohydrates on dual-species biofilms of S. mutans and A. naeslundii – a pilot study

Cited by 2 publications

References 25 publications

Examination of the Structure and Formation Streptococcus mutans Biofilm Induced by Glucose, Lactose, Soy Protein, and Iron

Examination of the Structure and Formation Streptococcus mutans Biofilm Induced by Glucose, Lactose, Soy Protein, and Iron

Effects of Sucrose and Farnesol on Biofilm Formation by Streptococcus mutans and Candida albicans

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