Influence of different energy densities of laser phototherapy on oral wound healing

Abstract

Abstract. The aim of the present prospective study was to evaluate the impact of laser phototherapy (LPT) on the healing of oral ulcers. Different power densities were used on oral wounds in Wistar rats (n ¼ 72) randomly divided into three groups: control (0 J∕cm 2 ), 4 J∕cm 2 laser, and 20 J∕cm 2 laser. Ulcers (3 mm in diameter) were made on the dorsum of the tongue with a punch. Irradiation with an indium-gallium-aluminum-phosphide laser (660 nm; output power: 40 mW; spot size: 0.04 cm 2 ) was performed once a day in close contact with the ulcer for 14 consecutive days. A statistically significant acceleration in healing time was found with wounds treated with 4 J∕cm 2 LPT. Moreover, striking differences were found in the ulcer area, healing percentage, degree of reepithelialization, and collagen deposition. The most significant changes occurred after 5 days of irradiation. Based on the conditions employed in the present study, LPT is capable of accelerating the oral mucosa wound-healing process. Moreover, faster and more organized reepithelialization and tissue healing of the oral mucosa were achieved with an energy density of 4 J∕cm 2 in comparison to 20 J∕cm 2 .

Photobiomodulation reduces oral mucositis by modulating NF-kB

“…NF-kB is a dimeric transcription factor formed by members of a family of proteins that share a conserved N-terminal dimerization/DNA-binding region known as the Rel homology domain. 13,14,16 Moreover, NF-kB activation has been implicated in survival responses of epithelial cells and increased angiogenesis mechanisms that involve VEGF expression. In the nucleus, NF-kB dimers bind to a kappa B site in the promoter or enhancer region of target genes, thereby controlling the expression of several genes involved in the inflammatory response.…”

“…The inclusion of PBM in current oral mucositis guideline is based on several clinical evidences supporting the use for prevention and treatment of oral mucositis. [12][13][14][15][16] The use of PBM is safe without side effects. 10,11 Furthermore, PBM improves cell proliferation and migration and transcription of genes involved in wound healing.…”

Comparing the effect of diode laser against acyclovir cream for the treatment of herpes labialis

“…The laser phototherapy has analgesic and anti-inflammatory properties and stimulates tissue regeneration, fibroblast proliferation and neo-vascularization potential (6). …”

LED Phototherapy with Gelatin Sponge Promotes Wound Healing in Mice

“…Also, photobiomodulation may increase the immune expression of collagen Type I during tissue repair and improve the quality of newly formed tissue in the presence of hypothyroidism . Photobiomodulation may also promote cell metabolism; induce analgesia, anti‐inflammatory action, tissue repair; and stimulate neovascularization and the early formation of collagen fibers .…”

“…Red or near infrared light promotes tissue repair, while blue light, such as that at 470 nm, is known to be antimicrobial, yet not impairing of wound healing with anti‐inflammatory in vitro . Light at 660 nm could accelerate the oral mucosa wound‐healing process with an energy density of 4 J cm −2 in comparison with 20 J cm −2 . Blue light at 415 nm with energy higher than 100 J cm −2 was shown to be efficacious in eradicating infection and increasing the survival rates of animal models with wound infections .…”