Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids

Sidler-Lauff, K; Boos, Alois; Kraenzlin, Marius E.; Liesegang, Annette

doi:10.2527/jas.2009-2682

Cited by 27 publications

(24 citation statements)

References 51 publications

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“…This same study described the age-dependent relationship of calbindin-D 9k expression. The responsiveness of calbindin-D 9k to calcitriol was not confirmed in the bovine intestine (39) or bovine rumen (46) , but more recent data report calbindin-D 9k responses to calcitriol in the duodenum and rumen of goats (26) . Ca channels also present differences in regulation.…”

Section: Adaptive Mechanisms Of Calcium Homeostasismentioning

confidence: 99%

“…Active ruminal absorption of Ca has been demonstrated in vitro (23) ; however, in vivo validation (24) and the quantification of the significance of this effect (25) have not consistently been confirmed in ruminants. The latest data from goats suggest that active Ca absorption can occur simultaneously in the rumen and the intestine (26) . Hence, the role in Ca regulation of the postruminal gastrointestinal segments, and by extension, in the overall gastrointestinal tract is assumed to be comparable with that of singlestomached species.…”

Section: General Definitionmentioning

confidence: 99%

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A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle

Martín-Tereso

Verstegen

2011

Nutr. Res. Rev.

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Most dairy cows exhibit different degrees of hypocalcaemia around calving because the gestational Ca requirements shift to the disproportionately high Ca requirements of lactation. Ca homeostasis is a robust system that effectively adapts to changes in Ca demand or supply. However, these adaptations often are not rapid enough to avoid hypocalcaemia. A delay in the reconfiguration of intestinal Ca absorption and bone resorption is probably the underlying cause of this transient hypocalcaemia. Several dietary factors that affect different aspects of Ca metabolism are known to reduce the incidence of milk fever. The present review describes the interactions between nutrition and Ca homeostasis using observations from cattle and extrapolations from other species and aims to quantitatively model the effects of the nutritional approaches that are used to induce dry cows into an early adaptation of Ca metabolism. The present model suggests that reducing dietary cation -anion difference (DCAD) increases Ca clearance from the blood by dietary induction of systemic acidosis, which results in hypercalciuria due to the loss of function of the renal Ca transient receptor potential vanilloid channel TRPV5. Alternatively, reducing the gastrointestinal availability of Ca by reducing dietary Ca or its nutritional availability will also induce the activation of Ca metabolism to compensate for basal blood Ca clearance. Our model of gastrointestinal Ca availability as well as blood Ca clearance in the transition dairy cow allowed us to conclude that the most common dietary strategies for milk fever prevention may have analogous modes of action that are based on the principle of metabolic adaptation before calving.

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Section: Adaptive Mechanisms Of Calcium Homeostasismentioning

confidence: 99%

Section: General Definitionmentioning

confidence: 99%

A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle

Martín-Tereso

Verstegen

2011

Nutr. Res. Rev.

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“…It is well documented that the small intestine is the main site of active intestinal Ca 2+ absorption in many species [4,15,18]; however, marked variations between different species have been observed [17,30-32]. …”

Section: Discussionmentioning

confidence: 99%

Expression patterns of intestinal calcium transport factors and ex-vivo absorption of calcium in horses

et al. 2011

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BackgroundIn many species, the small intestine is the major site of calcium (Ca2+) absorption. The horse differs considerably from most other species with regard to the physiology of its Ca2+ metabolism and digestion. Thus, this study was performed to get more information about the transcellular Ca2+ absorption in the horse.Two mechanisms of intestinal Ca2+ absorption are described: the passive paracellular pathway and the active, vitamin D-dependent transcellular pathway. The latter involves the following elements: vitamin D receptors (VDR), transient receptor potential vanilloid channel members 5 and 6 (TRPV5/6), calbindin-D9k (CB), the Na/Ca exchanger (NCX1) and the plasma membrane Ca-ATPase (PMCA). The aim of the present study was to investigate the protein and mRNA expression patterns of VDR, CB and TRPV6 and the ex-vivo Ca2+ absorption in horses, assessed by qualitative and quantitative RT-PCR, western blot, immunohistochemistry and the Ussing chamber technique.ResultsHighest CB and TRPV6 mRNA levels were detected in the duodenum as compared to the middle parts of the jejunum and ileum and several sites of the large intestine. VDR mRNA levels did not change significantly throughout the intestine. TRPV5 mRNA was not detectable in the horse intestine. The highest VDR and CB protein levels were measured in the duodenum. Ussing chamber studies revealed ex-vivo Ca2+ absorption only in the duodenum, but not in cecum and specific sites of the colon.ConclusionThe present findings suggest that TRPV6, CB and VDR may be involved in active intestinal Ca2+ absorption in horses, as described for other mammals. TRPV5 may not play a major role in this process. Furthermore, the expression patterns of these Ca2+ transport elements and the results of the Ussing chamber procedure indicate that a significant part of active intestinal Ca2+ absorption occurs in the duodenum in this species.

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“…The disease is known to develop in herbivores fed diets with a Ca:P ratio of 0.8 or lower (13,20). Other factors that may play a role include vitamin D deficiency (in housed animals), reduced calcium absorption due to endoparasitism, or calcium binding substances such as oxalate in the diet (13,19). The exact nature of the nutritional abnormality in this study was not determined but nutritional etiology supported by soil analysis and responsive Ca treatment.…”

Section: Discussionmentioning

confidence: 99%

Clinical and pathological studies on nutritional fibrous osteodystrophy in goats

Özmen¹,

Şahinduran²

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:Fibrous osteodystrophy is a metabolic disease that characterized marked bone resorption with fibrous tissue proliferations, cysts formations and insufficient mineralization of immature bone. Nutritional fibrous osteodystrophy was developed in 37 goats from two different goat flocks in Isparta in Turkey. The aim of this study was to examine clinicopathological findings and treatment of this rare disease. Affected animals were between 2-4 months of age and were normal at birth. Clinical signs were started about 2 months of age and characterized by mandibular and maxillary enlargements, softening of the bones (especially facial bones), varying degrees of mouth opening and protruding tongue, dyspnea, leg deformities, exophthalmos, and mastication problems. Exophthalmos was seen in 13 kids due to enlargement of facial bones into the cavum orbita. Leg deformities were observed in 11 kids and they had walking problems. Blood calcium, phosphorus, magnesium, alkaline phosphatase, vitamin D3 and parathormone levels were examined. Affected animals had increased levels of phosphorus and parathormone, as well as higher alkaline phosphatase activity. 23 kids died from the disease and 10 of them were necropsied. At necropsy, bilateral enlargement of the maxilla and mandibula, leg deformities and incomplete rib fractures were observed in kids. Histopathologically, severe diffuse proliferation of loose connective tissue surrounded the osteoid trabeculae, many of which were partially or completely non mineralized and accompanied by numerous osteoclastic giant cells. Soil analysis of the pasture revealed marked decrease in calcium level (1%). The newly born and under the one month of age kids in the flocks (totally 527 goat kids) were treated with Ca and Vit D. No new cases were observed after treatment.Keywords: Blood biochemistry, goat, nutritional fibrous osteodystrophy, pathology, soil analysis. Keçilerde nutrisyonel fibröz osteodistrofide klinik ve patolojik incelemelerÖzet: Fibröz osteodistrofi belirgin kemik rezorpsiyonu ile birlikte fibröz bağ doku proliferasyonları, kist formasyonları ve olgunlaşmamış kemik dokunun yetersiz mineralizasyonu ile karakterize metabolik bir hastalıktır. Isparta ilinde iki farklı keçi sürüsündeki 37 oğlakta nutrisyonel fibröz osteodistrofi saptandı. Bu çalışmanın amacı, keçilerde nadir rastlanan fibröz osteodistrofideki klinikopatolojik bulgular ile bu hastalığın tedavisinin incelenmesidir. Hasta hayvanlar 2-4 aylıktı ve doğduklarında normal görünümdeydiler. Klinik belirtiler hayvanlar yaklaşık 2 aylıkken başlayıp; mandibular ve maksiller genişlemeler, kemiklerde (özellikle yüz kemikleri) yumuşama, ağzın değişik derecelerde açık kalması ve dilin dışarıya sarkması, nefes darlığı, bacak deformiteleri, ekzoftalmus ve çiğneme problemleri ile karakterize bir tablo ile seyrediyordu. 13 oğlakta yüz kemiklerinin orbita çukuruna doğru genişlemesi sebebiyle ekzoftalmus şekillenmişti. 11 oğlakta bacak deformiteleri oluşmuştu ve bu hayvanlarda yürüme problemleri gözlendi. Kan kalsiyum, fosfor, magn...

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Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids

Cited by 27 publications

References 51 publications

A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle

A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle

Expression patterns of intestinal calcium transport factors and ex-vivo absorption of calcium in horses

Clinical and pathological studies on nutritional fibrous osteodystrophy in goats

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