Influence of dietary sodium alginate and Pediococcus acidilactici on liver antioxidant status, intestinal lysozyme gene expression, histomorphology, microbiota, and digestive enzymes activity, in Asian sea bass (Lates calcarifer) juveniles

Ashouri, Ghasem; Soofiani, Nasrollah Mahboobi; Hoseinifar, Seyed Hossein; Jalali, Seyed Amir Hossein; Morshedi, Vahid; Valinassab, Tooraj; Bagheri, Dara; Doan, Hien Van; Mozanzadeh, Mansour Torfi; Carnevali, Oliana

doi:10.1016/j.aquaculture.2019.734638

Cited by 38 publications

(27 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to histological observations, probiotic treatment improved significantly various aspects of intestinal histoarchitecture such as villus height and apparent muscular layer thickness, especially at higher doses, as compared to the control group. These findings are consistent with those of Zheng et al [99], Won et al [10], and Ashouri et al [100] who detected also further improvement in villus width and apparent villus surface as well as crypt depth. The improvement in intestinal histology in probiotic treatments could be attributable to an increase in intestinal surface area, thus increasing absorption surface that is associated with improvement in integrity of brush borders which would promote nutrient absorption and, in turn, improve growth performance, feed consumption, and survival [100][101][102][103].…”

Section: Discussionsupporting

confidence: 92%

Assessing the Influence of Dietary Pediococcus acidilactici Probiotic Supplementation in the Feed of European Sea Bass (Dicentrarchus labrax L.) (Linnaeus, 1758) on Farm Water Quality, Growth, Feed Utilization, Survival Rate, Body Composition, Blood Biochemical Parameters, and Intestinal Histology

Eissa

Baghdady

Gaafar

et al. 2022

Aquaculture Nutrition

View full text Add to dashboard Cite

The probiotics are being used as ecofriendly and bioremediation tools for developing sustainability to aquaculture. The present study was conducted to explore the practical capability of using dietary lactic acid bacteria (Pediococcus acidilactici) probiotics and see how its dose variation affected the water quality, growth performance, survival rate, body composition, blood biochemical parameters, and intestinal histology of European sea bass (Dicentrarchus labrax L.). A total of 120 fingerlings with an initial weight of 9 ± 0.2 g were divided into four groups, each with three replicates. The feeding experiment lasted for 60 days. In addition to the control (without probiotics) (T0), fish were fed diets containing (T1) 2.0, (T2) 2.5, and (T3) 3.0 g of probiotics per kg of diet twice a day. When compared to the control, sea bass fed probiotic-supplemented diets had significantly higher growth parameters, fish body “crude lipid,” and villi height ( p < 0.05 , p < 0.01 , and p < 0.001 ). The P. acidilactici probiotic treatments improved survival rate, feed conversion ratio, body composition, and blood biochemical markers, but not statistically significant ( p > 0.05 ). Also, in regard to water quality, P. acidilactici drastically reduced ammonia and pH levels. In this experiment, fish fed with a dosage of 3.0 g of this commercial probiotic per kg of probiotics performed better. The study found that including probiotics in the diets of European sea bass improved growth, body composition, survival rate, blood biochemical markers, intestinal histology, and some water quality parameters.

show abstract

Section: Discussionsupporting

confidence: 92%

Assessing the Influence of Dietary Pediococcus acidilactici Probiotic Supplementation in the Feed of European Sea Bass (Dicentrarchus labrax L.) (Linnaeus, 1758) on Farm Water Quality, Growth, Feed Utilization, Survival Rate, Body Composition, Blood Biochemical Parameters, and Intestinal Histology

Eissa

Baghdady

Gaafar

et al. 2022

Aquaculture Nutrition

View full text Add to dashboard Cite

show abstract

“…Previous studies show that the proliferation of LAB in the intestine of fish suppresses the colonization of Gram-negative pathogenic bacteria ( Kishawy et al, 2020 ). Moreover, aqua-feeds supplemented with LAB have been reported to stimulate the growth, enhance the disease resistance, effectively regulate the gut microbiota ( Xia et al, 2020 ), and stimulate immune responses in cultured aquatic species ( Ringo et al, 2018 ; Ashouri et al, 2020 ). LAB is expected to be growth promoters and immunostimulants in aquaculture ( LeBlanc et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Dietary Lactic Acid Bacteria on Intestinal Microbiota and Immune Responses of Crucian Carp (Carassius auratus) Under Water Temperature Decrease

Liu

Xu³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Temperature changes have a great impact on fish feeding, intestinal microorganisms, metabolism, and immune function. Therefore, it is necessary to develop effective methods to enhance the survival rates and growth of fish under water temperature changes. Lactic acid bacteria (LAB) are promising immunostimulatory feed additive, as demonstrated by their beneficial effects in several fish species. This study investigated the short-term effects of dietary LAB on intestinal microbiota composition and immune responses of crucian carp (Carassius auratus) when water temperature decreased from 30 ± 1°C to 18 ± 1°C. Lactococcus (L.) lactis 1,209 and L. lactis 1,242 with potential probiotics isolated from the intestine of Qinghai naked carp (Gymnocypris przewalskii) were selected as feed additives for the crucian carp feeding experiment. A total of 225 commercially available healthy crucian carp (250 ± 10 g) of similar age were kept in 30°C water for a week and then immediately transferred to 18 ± 1°C water, assigned to three dietary treatments for a 16-day feeding trial randomly: (1) HC, diets without additives (the control group); (2) HT, diets with 106 CFU/ml L. lactis 1,209; and (3) HL, with 106 CFU/ml L. lactis 1,242. Each group was set up with 3 replicates and each with 25 fish. The results showed that the mortality rate of crucian carp in HC, HT, and HL group was 50, 27, and 33%, respectively. High-throughput sequencing results displayed that the composition of the intestinal microorganism varied dynamically in response to different treatments and water temperature decrease. Among them, compared with the HC group, a higher abundance of Firmicutes and Proteobacteria, and a lower of Actinobacteria appeared in HT and HL. The cytokines heat shock protein 70 (HSP-70) in crucian carp intestinal tract significantly decreased when water temperature decreased (p < 0.05).

show abstract

“…At around 0.3 ppm DO level, L. calcalifer fed with alginate was still survived. Alginate performance can be improved by combining low molecular weight sodium alginate and Pediococcus acidilactici bacteria separately or in symbiotic form, with promising results as functional feed additives in juvenile Asian sea bass, L. calcarifer (Ashouri et al, 2020). Due to the sustainable on the availaibility of seaweed, especially Sargassum sp.…”

Section: Hematocrit and Hemoglobinmentioning

confidence: 99%

“…Sodium alginate (40.34% + 0,21) was the highest extract of alginate from Sargassum sp, followed by acid alginate (11.51% + 0,15) and calcium alginate (4.8% + 0,09) (Yudiati & Isnansetyo, 2017). Some research approves that raw (Zeynali et al, 2020), extract (Talpur, 2014), and Alginate (Ashouri et al, 2020) from Sargassum sp. were managed to increase the health status of L. calcarifer with oral administration.…”

Section: Introductionmentioning

confidence: 97%

Alginate from Sargassum sp. Improve the Hematology Performance and Oxygen Tolerance Exposure of Lates calcarifer

et al. 2022

View full text Add to dashboard Cite

Indonesia's fish production is abundant, especially in aquaculture. Lates calcarifer is farmed fish species. The failure of L. calcarifer cultivation due to disease problems. We are utilizing a natural compound derived from tropical Sargassum sp. extracts, namely alginate. This study aims to determine and analyse the supplementation of alginate in the diet by oral administration to improve the fish's hematological performance and oxygen stress tolerance. There were one control and three treatments (2 g.kg-1, 4 g.kg-1, and 8 g.kg-1 alginate/feed). The experiment was a completely randomized design with three replications. Fish were reared in a 350 L fiber tank for 12 days at a density of 20 ind.tank-1. Stress oxygen tolerance was applied by rearing the 10 fish in 12 L fully plastic-wrapped containers. The parameters test analyzed were phagocytic activity and phagocytic index, red blood parameters which are hematocrit and hemoglobin. The survival rate after 5 hr anoxic exposure was also recorded. The best treatments were achieved at a dose of 6 g.kg-1 and 8 g.kg-1in all parameters, except the phagocytic index. The alginate addition at all oxygen stress treatments also performed a better survival rate compared to the control. Adding alginate to feed as feed supplementation by oral administration can boost hematological performance and higher tolerance from oxygen stress exposure.

show abstract

Influence of dietary sodium alginate and Pediococcus acidilactici on liver antioxidant status, intestinal lysozyme gene expression, histomorphology, microbiota, and digestive enzymes activity, in Asian sea bass (Lates calcarifer) juveniles

Cited by 38 publications

References 103 publications

Assessing the Influence of Dietary Pediococcus acidilactici Probiotic Supplementation in the Feed of European Sea Bass (Dicentrarchus labrax L.) (Linnaeus, 1758) on Farm Water Quality, Growth, Feed Utilization, Survival Rate, Body Composition, Blood Biochemical Parameters, and Intestinal Histology

Assessing the Influence of Dietary Pediococcus acidilactici Probiotic Supplementation in the Feed of European Sea Bass (Dicentrarchus labrax L.) (Linnaeus, 1758) on Farm Water Quality, Growth, Feed Utilization, Survival Rate, Body Composition, Blood Biochemical Parameters, and Intestinal Histology

The Effect of Dietary Lactic Acid Bacteria on Intestinal Microbiota and Immune Responses of Crucian Carp (Carassius auratus) Under Water Temperature Decrease

Alginate from Sargassum sp. Improve the Hematology Performance and Oxygen Tolerance Exposure of Lates calcarifer

Contact Info

Product

Resources

About