Influence of Dietary Inulin on Fecal Microbiota, Cardiometabolic Risk Factors, Eicosanoids, and Oxidative Stress in Rats Fed a High-Fat Diet

Miralles-Pérez, Bernat; Nogués, María Rosa; Sánchez-Martos, Vanessa; Fortuño-Mar, Angeles; Ramos-Romero, Sara; Torres, Josep Lluís; Ponomarenko, Julia; Amézqueta, Susana; Zhang, Xiang; Romeu, Marta

doi:10.3390/foods11244072

Cited by 5 publications

(4 citation statements)

References 88 publications

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“…While HbA1c levels decreased proportionally with increases in inulin DPn, the changes were not statistically significant, possibly due to the limitations posed by the statistical approaches utilized and the short duration of the study. This view is supported by a study conducted by Miralles-Pérez et al, demonstrating that inulin (15% w/w of diet) reduced HbA1c levels in HFD-rats after 10 weeks [46].…”

Section: Metabolic and Inflammatory Biomarker Responses To Inulin Sup...mentioning

confidence: 69%

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The Degree of Inulin Polymerization Is Important for Short-Term Amelioration of High-Fat Diet (HFD)-Induced Metabolic Dysfunction and Gut Microbiota Dysbiosis in Rats

Ariaee,

Wardill,

Wignall

et al. 2024

Foods

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Inulin, a non-digestible polysaccharide, has gained attention for its prebiotic properties, particularly in the context of obesity, a condition increasingly understood as a systemic inflammatory state linked to gut microbiota composition. This study investigates the short-term protective effects of inulin with different degrees of polymerization (DPn) against metabolic health deterioration and gut microbiota alterations induced by a high-fat diet (HFD) in Sprague Dawley rats. Inulin treatments with an average DPn of 7, 14, and 27 were administered at 1 g/kg of bodyweight to HFD-fed rats over 21 days. Body weight, systemic glucose levels, and proinflammatory markers were measured to assess metabolic health. Gut microbiota composition was analyzed through 16S rRNA gene sequencing. The results showed that inulin27 significantly reduced total weight gain and systemic glucose levels, suggesting a DPn-specific effect on metabolic health. The study also observed shifts in gut microbial populations, with inulin7 promoting several beneficial taxa from the Bifidobacterium genera, whilst inducing a unique microbial composition compared to medium-chain (DPn 14) and long-chain inulin (DPn: 27). However, the impact of inulin on proinflammatory markers and lipid metabolism parameters was not statistically significant, possibly due to the short study duration. Inulin with a higher DPn has a more pronounced effect on mitigating HFD-induced metabolic health deterioration, whilst inulin7 is particularly effective at inducing healthy microbial shifts. These findings highlight the benefits of inulin as a dietary adjuvant in obesity management and the importance of DPn in optimizing performance.

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Section: Metabolic and Inflammatory Biomarker Responses To Inulin Sup...mentioning

confidence: 69%

“…13, x FOR PEER REVIEW 6 of 18supported by a study conducted by Miralles-Pérez et al, demonstrating that inulin (15% w/w of diet) reduced HbA1c levels in HFD-rats after 10 weeks[46].…”

mentioning

confidence: 94%

The Degree of Inulin Polymerization Is Important for Short-Term Amelioration of High-Fat Diet (HFD)-Induced Metabolic Dysfunction and Gut Microbiota Dysbiosis in Rats

Ariaee,

Wardill,

Wignall

et al. 2024

Foods

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“…This analysis would have made it possible to better understand the impact of changes in gut bacterial abundance on the effect of inulin on skeletal muscle. Of note, the modification of the gut microbiota induced by a diet enriched with inulin has already been described in rats in different conditions, e.g., standard condition, obesity [63,64]. Inulin is usually regarded as a type of prebiotic, favorably stimulating the growth of Bifidobacteria lactobacilli and actinobacteria and inhibiting the growth of bacteroidetes.…”

Section: Discussionmentioning

confidence: 99%

Associating Inulin with a Pea Protein Improves Fast-Twitch Skeletal Muscle Mass and Muscle Mitochondrial Activities in Old Rats

Salles,

Gueugneau,

Patrac

et al. 2023

Nutrients

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Aging is associated with a decline in muscle mass and function, leading to increased risk for mobility limitations and frailty. Dietary interventions incorporating specific nutrients, such as pea proteins or inulin, have shown promise in attenuating age-related muscle loss. This study aimed to investigate the effect of pea proteins given with inulin on skeletal muscle in old rats. Old male rats (20 months old) were randomly assigned to one of two diet groups for 16 weeks: a ‘PEA’ group receiving a pea-protein-based diet, or a ‘PEA + INU’ group receiving the same pea protein-based diet supplemented with inulin. Both groups showed significant postprandial stimulation of muscle p70 S6 kinase phosphorylation rate after consumption of pea proteins. However, the PEA + INU rats showed significant preservation of muscle mass with time together with decreased MuRF1 transcript levels. In addition, inulin specifically increased PGC1-α expression and key mitochondrial enzyme activities in the plantaris muscle of the old rats. These findings suggest that dietary supplementation with pea proteins in combination with inulin has the potential to attenuate age-related muscle loss. Further research is warranted to explore the underlying mechanisms and determine the optimal dosage and duration of intervention for potential translation to human studies.

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“…Dysregulation of antioxidant homeostasis is associated with the occurrence of many diseases, while increased levels of antioxidant enzymes can effectively repair DNA damage, and may reduce or alleviate the onset of chronic diseases [37], such as chronic obstructive pulmonary disease [38], atherosclerosis of the arteries [39] and chronic kidney disease [40]. Numerous studies have proven that dietary inulin [41,42] and VD [43,44] addition can improve immunity and anise oxidative stress through activation of Nrf2 and its downstream antioxidant enzyme system, including GSH-px, SOD, CAT, etc. In addition to alleviating oxidative stress, the activation of Nrf2 can also regulate the inflammatory state and reduce the occurrence of ferroptosis, which plays an important role in inflammation [45], cancer [46], aging [47], cell damage [48], fertility [49] and other aspects.…”

Section: Discussionmentioning

confidence: 99%

Maternal Vitamin D and Inulin Supplementation in Oxidized Oil Diet Improves Growth Performance and Hepatic Innate Immunity in Offspring Mice

et al. 2023

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Dietary oxidized fat contains harmful materials such as hydrogen peroxide and malondialdehyde (MDA). Excessive oxidized fat intake during pregnancy and lactation not only leads to maternal body injury but also damages offspring health. Our previous study demonstrated that vitamin D (VD) had antioxidative capability in sows. This study was conducted to investigate the effect of maternal VD and inulin supplementation in oxidized oil diet on the growth performance and oxidative stress of their offspring. Sixty 5-month-old C57BL/6N female mice were randomly divided into five groups: Control group (basal diet, n = 12), OF group (oxidized-soybean-oil-replaced diet, n = 12), OFV group (oxidized-soybean-oil-replaced diet + 7000 IU/kg VD, n = 12), OFI group (oxidized-soybean-oil-replaced diet + 5% inulin, n = 12) and OFVI group (oxidized-soybean-oil-replaced diet + 7000 IU/kg VD + 5% inulin, n = 12). Mice were fed with the respective diet during pregnancy and lactation. The offspring were then slaughtered on day 21 of age at weaning. Results showed that a maternal oxidized oil diet impaired body weight and liver weight gain of offspring during lactation compared to the control group, while maternal VD, inulin or VD and inulin mixture supplementation reversed this effect. In addition, the activity of T-AOC in the liver of offspring was lower in the OF group than that in the control group, but could be restored by maternal VD and inulin mixture supplementation. Furthermore, the gene expression of both proinflammatory and anti-inflammatory cytokines, such as Il-6, Tnfα and Il-10, in offspring liver were downregulated by a maternal oxidized oil diet compared with the control group, but they were restored by maternal VD or VD and inulin mixture supplementation. The expressions of Vdr and Cyp27a1 were decreased by a maternal oxidized oil diet compared with the control group, while they could be increased by VD or VD and inulin mixture supplementation. Conclusion: maternal oxidized oil diet intake could impair the growth performance by inducing oxidative stress, but this can be relieved by maternal VD and inulin supplementation.

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Influence of Dietary Inulin on Fecal Microbiota, Cardiometabolic Risk Factors, Eicosanoids, and Oxidative Stress in Rats Fed a High-Fat Diet

Cited by 5 publications

References 88 publications

The Degree of Inulin Polymerization Is Important for Short-Term Amelioration of High-Fat Diet (HFD)-Induced Metabolic Dysfunction and Gut Microbiota Dysbiosis in Rats

The Degree of Inulin Polymerization Is Important for Short-Term Amelioration of High-Fat Diet (HFD)-Induced Metabolic Dysfunction and Gut Microbiota Dysbiosis in Rats

Associating Inulin with a Pea Protein Improves Fast-Twitch Skeletal Muscle Mass and Muscle Mitochondrial Activities in Old Rats

Maternal Vitamin D and Inulin Supplementation in Oxidized Oil Diet Improves Growth Performance and Hepatic Innate Immunity in Offspring Mice

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