Influence of dietary fiber from coconut kernel (cocos nucifera) on the 1,2-dimethylhydrazine-induced lipid peroxidation in rats

Pillai, Manoj G; Thampi, B S Harikumaran; Menon, Venugopal P.; Leelamma, S

doi:10.1016/s0955-2863(99)00035-2

Cited by 23 publications

(14 citation statements)

References 21 publications

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“…Experimentally, it has been depicted that decreased LPO is associated with enhanced GSH in the colon and intestines (Pillai et al, 1999). In this study, enhanced GSH levels following 16 weeks of DMH treatment have been observed.…”

Section: Effect On Reduced Glutathionesupporting

confidence: 55%

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Pioglitazone hydrochloride: chemopreventive potential and development of site-specific drug delivery systems

Sinha

Sethi

2014

Drug Delivery

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The aim of this study was to investigate the potential of pioglitazone hydrochloride as a promising anticancer agent and then to design and evaluate the colon-targeted delivery system. The role of pioglitazone hydrochloride as a promising anticancer agent was evaluated by in vitro cell line studies and in vivo 1,2-dimethylhydrazine-induced colon carcinogenesis in rats. In order to deliver the drug at site of action, i.e. colon, drug embedded in matrices containing a release retarding polymer (HPMC K4M) and a polysaccharide (locust bean gum) were prepared. These matrix systems were further enteric coated with Eudragit Õ S100 to minimize the premature drug release in the upper segments of the GIT. In vitro dissolution studies were performed in absence and presence of rat caecal contents on selected batches and samples were analyzed using a validated RP-HPLC method. Hence, the studies led to the conclusion that successful site-specific delivery systems of pioglitazone hydrochloride were developed to improve its therapeutic efficacy in the management of colorectal cancer.

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Section: Effect On Reduced Glutathionesupporting

confidence: 55%

“…Numerous studies have shown reduction in rates of LPO in the tumor tissue of various types of cancers (Tanaka et al, 1998;Pillai et al, 1999). Acquisition of particular characteristics by cancer cells has benefitted their proliferation (Schmelz et al, 2000).…”

Section: Effect On Lpomentioning

confidence: 99%

Pioglitazone hydrochloride: chemopreventive potential and development of site-specific drug delivery systems

Sinha

Sethi

2014

Drug Delivery

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“…While several hypotheses have been formulated about AhR upregulation, it is difficult to explain its downregulation. Less SOD activity and lower GSH concentration were also observed in rats supplemented with coconut fibers [Pillai et al, 1999]. Diminished erythrocytic activities for GPx and catalase were reported in rats after diet supplementation with Bacillus polyfermenticus [Paik et al, 2005].…”

Section: Discussionmentioning

confidence: 89%

Modulatory activity of a Lactobacillus casei strain on 1,2‐dimethylhydrazine‐induced genotoxicity in rats

Villarini

Caldini

Moretti

et al. 2008

Environ and Mol Mutagen

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The present study was designed to investigate the putative antigenotoxic effects of supplementing the diet of rats treated with the colon carcinogen 1,2-dimethylhydrazine hydrochloride (DMH) with a Lactobacillus casei strain using an in vivo approach. The antigenotoxic response was evaluated in colon and liver cells using the alkaline comet assay. Since the balance between the bioactivation and detoxification metabolic pathways is crucial for the formation of toxic and genotoxic metabolites, alterations in the level of some xenobiotic metabolizing enzymes (XME) were studied in liver preparations. In the challenge group (L. casei + DMH), lactobacilli-supplemented diet, there was a decrease in the extent of DMH-induced DNA damage, especially in colon cells. Compared with control rats, there was less basal DNA damage in colon cells of rats fed on a lactobacilli-supplemented diet. These findings are the first to give clear evidence of DNA-protective effects of lactobacilli against basal DNA damage. Moreover, the chemopreventive effects were accompanied by changes in the activities of several XME. The observed decrease in the concentration of nonenzymatic antioxidants (i.e. GSH) and the reduced activity of enzymatic antioxidants (i.e., GST, GPx, and SOD) in liver could reflect an overall reduction in the level of oxidative stress in rats on a diet supplemented with the L. casei suspension compared with control rats (basal state). Thus, the concentrations of GSH and the activities of GST, GPx, and SOD could be downregulated by supplementing the diet with L. casei as a response to an improved antioxidant status.

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“…The fall in catalase activity correlated well with tumor stage according to Dukes, suggesting that this peroxisomal enzyme could be used as a potential prognostic marker [36] . Decreased lipid peroxidation associated with enhanced GSH in the colon and intestines is a well known phenomenon in experimental carcinogenesis [37] . We have also observed enhanced GSH levels following 20 wk of DMH treatment.…”

Section: Discussionmentioning

confidence: 99%

Selenium as a chemopreventive agent in experimentally induced colon carcinogenesis

Ghadi

Ghara²,

Bhattacharyya³

et al. 2009

WJGO

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AIM:To elucidate the chemopreventive efficacy of selenium during experimentally induced colon carcinogenesis. METHODS:Thirty-two male wistar rats were divided into four groups: group Ⅰ (normal control); group Ⅱ [1,2-dimethylhydrazine (DMH) treated]; group Ⅲ (selenium treated); and group Ⅳ (DMH + selenium treated). Groups Ⅱ and Ⅳ were given subcutaneous injections of DMH (30 mg/kg body weight) every week for 20 wk. Selenium, in the form of sodium selenite, was given to groups Ⅲ and Ⅳ at 1 ppm in drinking water ad libitum for 20 wk. At the end of the study, rats were sacrificed and their colons were analyzed for the development of tumors, antioxidant enzyme levels and histological changes. RESULTS:100% of the DMH treated rats developed tumors, which was reduced to 60% upon simultaneous selenium supplementation. Similarly, tumor multiplicity decreased to 1.1 following selenium supplementation to DMH treated rats. Levels of lipid peroxidation, glutathione-S-transferase, superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) decreased following DMH treatment, whereas levels of glutathione (GSH) and glutathione reductase (GR) significantly increased in DMH treated rats. Selenium administration to DMH treated rats led to an increase in the levels of lipid peroxidation, SOD, catalase, glutathione-S-transferase and GPx, but decreased the levels of GSH and GR. Histopathological studies on DMH treated rats revealed dysplasia of the colonic histoarchitecture, which showed signs of improvement following selenium treatment. CONCLUSION:The study suggests the antioxidative potential of selenium is a major factor in providing protection from development of experimentally induced colon carcinogenesis.

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Influence of dietary fiber from coconut kernel (cocos nucifera) on the 1,2-dimethylhydrazine-induced lipid peroxidation in rats

Cited by 23 publications

References 21 publications

Pioglitazone hydrochloride: chemopreventive potential and development of site-specific drug delivery systems

Pioglitazone hydrochloride: chemopreventive potential and development of site-specific drug delivery systems

Modulatory activity of a Lactobacillus casei strain on 1,2‐dimethylhydrazine‐induced genotoxicity in rats

Selenium as a chemopreventive agent in experimentally induced colon carcinogenesis

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