Influence of deacetylation of chitin on fibroblast-like cells and osteoblasts.

Tominaga, Kazuhiro; Tominaga, Naohiro; Tokuhisa, Michio; Kifune, Koji; Umemoto, Toshihiko; Fukuda, Jinichi; Mizuno, Akio

doi:10.5794/jjoms.44.941

Cited by 3 publications

(5 citation statements)

References 3 publications

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“…For the culture time of 1 and 3 d, the optical density (OD) values of CHP were similar to that of CP. It has been stated that chitin is biocompatible and suitable for osteoblasts culture. ,− From the MTT results for 5 and 7 d, the cell viability increased obviously with the incorporation of HAP. HAP is one of the components of natural bone, so it is suitable for osteoblasts attachment and proliferation.…”

Section: Resultsmentioning

confidence: 99%

Biocompatible and Biodegradable Bioplastics Constructed from Chitin via a “Green” Pathway for Bone Repair

Wang

et al. 2017

ACS Sustainable Chem. Eng.

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Biodegradable plastics are urgently needed in the biomedical field to avoid the secondary surgery for implants after completing the repair of nonload bearing bone defects. Herein, novel chitin based plastics were successfully fabricated by changing the shape and aggregation state structure of chitin hydrogels through drying under a negative pressure, which led to a plastic deformation. The chitin hydrogels were prepared by using an environmentally friendly aqueous NaOH/urea solvent, and then radially oriented under the negative pressure to form chitin bioplastics (CP) on the basis of the removability of the chitin molecular bundles in the hydrogels. Moreover, hydroxyapatite (HAP) was in situ synthesized to obtain the chitin/HAP composite plastics (CHP). Their structure and properties were characterized by SEM, FTIR, 13 C NMR, X-ray diffraction and mechanical testing. The results indicated that the bioplastic preparation was a "green" physical process, and the incorporation of HAP reinforced significantly the tensile strength of CHP. The viability, biocompatibility, hemocompatibility and in vivo histocompatibility of the bioplastics were evaluated systematically. The introduction of HAP could improve the cell adhesion, proliferation and differentiation of the osteoblast cells. Moreover, CHP exhibited good histocompatibility, hemocompatibility and in vivo biodegradability, showing potential application in the bone tissue engineering field.

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Section: Resultsmentioning

confidence: 99%

Biocompatible and Biodegradable Bioplastics Constructed from Chitin via a “Green” Pathway for Bone Repair

Wang

et al. 2017

ACS Sustainable Chem. Eng.

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“…Chitosan membranes (Kuo et al ., ), nanofibres (Shin et al ., ) and sponges (Seol et al ., ) have been used as scaffolds for bone formation. However, chitin and chitosan change their physical and biological properties according to their acetylation levels (Tominaga et al ., ). Mouse osteoblasts were cultured on sheets made of acetylated chitin and 35%, 50% and 70% deacetylated chitin (DAC‐0, DAC‐35, DAC‐50 and DAC‐70, respectively).…”

Section: Discussionmentioning

confidence: 97%

“…Mouse osteoblasts were cultured on sheets made of acetylated chitin and 35%, 50% and 70% deacetylated chitin (DAC‐0, DAC‐35, DAC‐50 and DAC‐70, respectively). The biocompatibility of DAC‐35 was the highest, and DAC‐70 suppressed cell growth and differentiation (Tominaga et al ., ). The DAC‐80 chitosan was used to prepare PCH in the present study, and it might suppress the growth and differentiation of osteoblasts.…”

Section: Discussionmentioning

confidence: 97%

Ultraviolet light-irradiated photocrosslinkable chitosan hydrogel to prevent bone formation in both rat skull and fibula bone defects

Tsuda

Hattori

Tanaka

et al. 2012

J Tissue Eng Regen Med

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In the field of orthopaedic surgery, an orthopaedic surgeon sometimes requires to suppress excessive bone formation, such as ectopic bone formation, ossifying myositis and radio-ulnar synostosis, etc. Ultraviolet (UV) light irradiation of a photocrosslinkable chitosan (Az-CH-LA) generates an insoluble hydrogel within 30 s. The purpose of this study was to evaluate the ability of the photocrosslinked chitosan hydrogel (PCH) to inhibit bone formation in an experimental model of bone defect. Rat calvarium and fibula were surgically injured and PCH was implanted into the resultant bone defects. The PCH implants significantly prevented bone formation in the bone defects during the 4 and 8 week observation periods. In the PCH-treated defects, fibrous tissues infiltrated by inflammatory cells were formed by day 7, completely filling the bone defects. In addition to these findings, expression of osteocalcin and runt-related gene 2 (RUNX2) mRNA, both markers of bone formation, was lower in the PCH-treated defects than in the controls. In contrast, collagen type 1α2 and α-smooth muscle actin (α-SMA) mRNA levels were significantly higher in the PCH-treated defects after 1 week. PCH stimulated the formation of fibrous tissue in bone defects while inhibiting bone formation. Thus, PCH might be a promising new therapeutic biomaterial for the prevention of bone formation in orthopaedic surgery.

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“…Furthermore, chitosan membrane (10), nanofiber (11), and sponge (12) have been produced as tissue‐engineering scaffolds for bone formation. However, chitin (chitosan) changes its physical and biological properties according to its deacetylation level (13). When mouse osteoblast cells were cultured on sheets made of nondeacetylated chitin, and 35, 50, and 70% deacetylated chitin (DAC‐0, DAC‐35, DAC‐50, and DAC‐70, respectively), DAC‐35 had the highest effect of growth and differentiation of the cells, and DAC‐50 and DAC‐0 had intermediate effects, while DAC‐70 suppressed the growth and differentiation of the cells (13).…”

Section: Discussionmentioning

confidence: 99%

“…However, chitin (chitosan) changes its physical and biological properties according to its deacetylation level (13). When mouse osteoblast cells were cultured on sheets made of nondeacetylated chitin, and 35, 50, and 70% deacetylated chitin (DAC‐0, DAC‐35, DAC‐50, and DAC‐70, respectively), DAC‐35 had the highest effect of growth and differentiation of the cells, and DAC‐50 and DAC‐0 had intermediate effects, while DAC‐70 suppressed the growth and differentiation of the cells (13). In the present study, DAC‐80 chitin (chitosan) was used to prepare photocrosslinkable chitosan hydrogel.…”

Section: Discussionmentioning

confidence: 99%

Photocrosslinkable Chitosan Hydrogel Can Prevent Bone Formation in Both Rat Skull and Fibula Bone Defects

et al. 2009

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UV light irradiation to a photocrosslinkable chitosan (Az-CH-LA) resulted in an insoluble and flexible hydrogel within 30 s. The purpose of this study was to evaluate the ability of the photocrosslinkable chitosan to inhibit bone formation in the bone defects. A 5-mmdiameter defect was made in the rat calvarium, and then photocrosslinkable chitosan was implanted and irradiated with UV for 30 s. Furthermore, a 2-mm defect was made in the fibula of a rat hind leg, and then photocrosslinkable chitosan was implanted and irradiated with UV. Bone formations in the rat skull and fibula defects with photocrosslinkable chitosan hydrogel were significantly prevented for 8 weeks. Thus, the chitosan hydrogel has an inhibitory effect on bone formation.

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Influence of deacetylation of chitin on fibroblast-like cells and osteoblasts.

Cited by 3 publications

References 3 publications

Biocompatible and Biodegradable Bioplastics Constructed from Chitin via a “Green” Pathway for Bone Repair

Biocompatible and Biodegradable Bioplastics Constructed from Chitin via a “Green” Pathway for Bone Repair

Ultraviolet light-irradiated photocrosslinkable chitosan hydrogel to prevent bone formation in both rat skull and fibula bone defects

Photocrosslinkable Chitosan Hydrogel Can Prevent Bone Formation in Both Rat Skull and Fibula Bone Defects

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