Influence of cytochalasin B on oocyte maturation in Xenopus laevis

Ryabova, L. V.; Betina, M.I.; Vassetzky, S. G.

doi:10.1016/0045-6039(86)90065-5

Cited by 33 publications

(25 citation statements)

References 17 publications

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“…Chromosome misalignment indicated the failure of chromosome congression, which is also driven by actin. 26,28 These results are consistent with previous work showing that the disruption of actin can lead to defects in spindle formation 29,30 and with the known involvement of the actin nucleation regulators Cdc42, Rac, Formin, the Arp2/3 complex and JMY in spindle migration in mouse oocytes. 25,[31][32][33][34][35][36][37] WAVE2-mediated actin formation may regulate chromosome congression, spindle formation and migration.…”

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tsupporting

confidence: 82%

WAVE2 regulates meiotic spindle stability, peripheral positioning and polar body emission in mouse oocytes

Sun¹,

Xu²,

Li³

et al. 2011

Cell Cycle

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Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tsupporting

confidence: 82%

WAVE2 regulates meiotic spindle stability, peripheral positioning and polar body emission in mouse oocytes

Sun¹,

Xu²,

Li³

et al. 2011

Cell Cycle

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“…Similar results have been obtained in other species, such as hamster [172], cattle [173], pig [156] and human [174], while in Xenopus the results are contradictory [11,175,176]. …”

Section: Reproductionsupporting

confidence: 80%

Impact of Marine Drugs on Cytoskeleton-Mediated Reproductive Events

Silvestre

Tosti

2010

Marine Drugs

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Marine organisms represent an important source of novel bioactive compounds, often showing unique modes of action. Such drugs may be useful tools to study complex processes such as reproduction; which is characterized by many crucial steps that start at gamete maturation and activation and virtually end at the first developmental stages. During these processes cytoskeletal elements such as microfilaments and microtubules play a key-role. In this review we describe: (i) the involvement of such structures in both cellular and in vitro processes; (ii) the toxins that target the cytoskeletal elements and dynamics; (iii) the main steps of reproduction and the marine drugs that interfere with these cytoskeleton-mediated processes. We show that marine drugs, acting on microfilaments and microtubules, exert a wide range of impacts on reproductive events including sperm maturation and motility, oocyte maturation, fertilization, and early embryo development.

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“…Formation of this cytoplasmic actin is regulated during cell cycle. Similarly in Xenopus oocytes, although the regulation of cytoplasmic actin dynamics wholly remained to be clarified, actin filaments are also required for spindle migration and rotation [Ryabova et al, 1986;Gard et al, 1995]. Increase in cytoplasmic actin filaments at mitotic phase is also observed in C. elegans [Velarde et al, 2007].…”

Section: Discussionmentioning

confidence: 99%

“…Examination of the effect of jasplakinolide, which is known to stabilize actin filaments (an effect opposite to that of cytochalasins), has shown that this drug also prevents spindle migration to the murine oocyte cortex [Terada et al, 2000], suggesting that turnover and/or disassembly of actin filaments is required for spindle migration during oocyte maturation. Actin filaments are also essential for peripheral spindle anchorage in Xenopus and mammalian oocytes [Ryabova et al, 1986;Le Guen et al, 1989;Gard et al, 1995]. In Xenopus oocytes, myosin-10 (Myo10), a microtubule-binding actin-based motor protein, plays a critical role in spindle formation and anchorage; disruption of Myo10 function leads to severe defects in spindle microtubule assembly, spindle-actin filament association, and spindle anchorage [Weber et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

Stabilization of actin filaments prevents germinal vesicle breakdown and affects microtubule organization in Xenopus oocytes

et al. 2012

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In Xenopus oocytes, extremely giant nuclei, termed germinal vesicles, contain a large amount of actin filaments most likely for mechanical integrity. Here, we show that microinjection of phalloidin, an F-actin-stabilizing drug, prevents the germinal vesicle breakdown (GVBD) in oocytes treated with progesterone. These nuclei remained for more 12 h after control oocytes underwent GVBD. Immunostaining showed significant elevation of actin in the remaining nuclei and many actin filament bundles in the cytoplasm. Furthermore, microtubules formed unusual structures in both nuclei and cytoplasm of phalloidin-injected oocytes stimulated by progesterone. Cytoplasmic microtubule arrays and intranuclear microtubules initially formed in phalloidin-injected oocytes as control oocytes exhibited white maturation spots; these structures gradually disappeared and finally converged upon intranuclear short bundles when control oocytes completed maturation. In contrast, treatment of oocytes with jasplakinolide, a cell membrane-permeable actin filament-stabilizing drug, did not affect GVBD. This drug preferentially induced accumulation of actin filaments at the cortex without any increase in cytoplasmic actin staining. Based on these results, intranuclear and cytoplasmic actin filament dynamics appear to be required for the completion of GVBD and critically involved in the regulation of microtubule assembly during oocyte maturation in Xenopus laevis.

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Influence of cytochalasin B on oocyte maturation in Xenopus laevis

Cited by 33 publications

References 17 publications

WAVE2 regulates meiotic spindle stability, peripheral positioning and polar body emission in mouse oocytes

WAVE2 regulates meiotic spindle stability, peripheral positioning and polar body emission in mouse oocytes

Impact of Marine Drugs on Cytoskeleton-Mediated Reproductive Events

Stabilization of actin filaments prevents germinal vesicle breakdown and affects microtubule organization in Xenopus oocytes

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