Influence of Culture Parameters on Phenolics, Flavonoids and Antioxidant Activity in Cell Culture Extracts of Date Palm (Phoenix dactylifera L.)

Naik, Poornananda Madhava; Al-Khayri, Jameel M.

doi:10.1007/s10341-020-00469-y

Cited by 9 publications

(3 citation statements)

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“…The Folin–Ciocalteu method [ 30 ] was used to determine the TPC from date palm cell suspension culture, with some modifications [ 31 ]. Methanolic extract (20 µL) was mixed with 1.58 mL of deionized water and 100 µL of Folin–Ciocalteu reagent (SRL, Mumbai, India, AR grade); then, it was allowed to stand for 8 min before 20% of 300 µL sodium carbonate solution (HiMedia, Mumbai, India, AR grade) was added.…”

Section: Methodsmentioning

confidence: 99%

“…The TPC of cell extract was expressed as mg GAE/100 g of DW. The colorimetric method as explained by Thiruvengadam et al [ 32 ] was used to determine the TFC of date palm cell suspension culture extracts, with some modifications [ 31 ]. Methanolic extracts (100 µL), 10% of 50 µL aluminum chloride (SRL, Mumbai, India, AR grade), 1 M of 50 µL potassium acetate (HiMedia, Mumbai, India, AR grade), and distilled water (1.8 mL) were mixed.…”

Section: Methodsmentioning

confidence: 99%

“…The stable free radical 2,2-diphenyl-1-picrylhydrazyl [DPPH, (SRL, Mumbai, India, AR grade)] was used to test the antioxidant activities of a particular compound/cell extract due to its hydrogen ion-donating ability [ 33 ]. The antioxidant activity of date palm cell extracts was determined by referring to the Farag et al [ 3 ] protocol with some modifications [ 31 ]. The original cell extract (sample) in methanol made a stock value of 1 mg/mL.…”

Section: Methodsmentioning

confidence: 99%

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Elicitor-Induced Production of Biomass and Pharmaceutical Phenolic Compounds in Cell Suspension Culture of Date Palm (Phoenix dactylifera L.)

Al-Khayri

Naik

2020

Molecules

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Plants that synthesize bioactive compounds that have high antioxidant value and elicitation offer a reliable in vitro technique to produce important nutraceutical compounds. The objective of this study is to promote the biosynthesis of these phenolic compounds on a large scale using elicitors in date palm cell suspension culture. Elicitors such as pectin, yeast extract (YE), salicylic acid (SA), cadmium chloride (CdCl2), and silver nitrate (AgNO3) at 50, 100, and 200 mg/L concentrations are used. The effects of elicitors on cell culture were determined in terms of biomass [packed cell volume (PCV), fresh and dry weight], antioxidant activity, and phenolic compounds (catechin, caffeic acid, kaempferol, apigenin) were determined using high-performance liquid chromatography (HPLC). Results revealed that enhanced PCV (12.3%), total phenolic content [317.9 ± 28.7 mg gallic acid equivalents (GAE)/100 g of dry weight (DW)], and radical scavenging activity (86.0 ± 4.5%) were obtained in the 50 mg/L SA treated cell culture of Murashige and Skoog (MS) medium. The accumulation of optimum catechin (26.6 ± 1.3 µg/g DW), caffeic acid (31.4 ± 3.8 µg/g DW), and kaempferol (13.6 ± 1.6 µg/g DW) was found in the 50 mg/L SA-treated culture when compared to the control. These outcomes could be of great importance in the nutraceutical and agronomic industries.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%