Influence of culture conditions on biofilm formation by Escherichia coli O157:H7

Dewanti, Ratih; Wong, Amy C. Lee

doi:10.1016/0168-1605(94)00103-d

Cited by 238 publications

(145 citation statements)

References 36 publications

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“…Instead, bacteria tend to form biofilms under low-nutrient or starvation conditions (17,27,97,130). For instance, Dewanti and Wong found that Escherichia coli O157:H7 biofilms developed on stainless steel chipped faster and contained a higher number of adherent cells when grown in low-nutrient media than when grown in tryptic soy broth (36). The attachment-upon-starvation response has also been commonly observed for various bacteria, including pathogenic bacteria as well as those found in soil and the marine environment (4,27,65,80,81,97,134).…”

Section: Why Attach In the First Place?mentioning

confidence: 99%

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Petrova

Sauer

2012

Journal of Bacteriology

319

251

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Section: Why Attach In the First Place?mentioning

confidence: 99%

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Petrova

Sauer

2012

Journal of Bacteriology

319

251

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“…Although E. coli is reported to be destroyed by pasteurisation, there are reports on its ability (including the pathogenic strain O157:H7) to form biofilms within pasteurisation equipment, leading to pasteurisation failures [8,39]. Listeria monocytogenes [9] and Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

Impact of ultrasound on dairy spoilage microbes and milk components

Cameron

McMaster

Britz

2009

Dairy Sci. Technol.

131

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-Numerous reports in the literature suggest pasteurisation failures in the dairy industry as a possible cause for an end product with a poor quality. Ultrasonication offers the dairy industry a non-thermal alternative to pasteurisation. The aim of this study was to evaluate the use of ultrasonication as an alternative to heat pasteurisation. Ultrasound was found to eliminate spoilage and potential pathogens to zero or to levels acceptable by South African and British milk legislation, even when initial inoculum loads of 5× higher than permitted were present before treatment. Viable cell counts of E. coli were reduced by 100% after 10.0 min of ultrasonication. The data obtained also showed that viable counts of Pseudomonas fluorescens were reduced by 100% after 6.0 min and Listeria monocytogenes was reduced by 99% after 10.0 min. An infra-red based apparatus was used to analyse raw and pasteurised milk after an ultrasonic treatment. Ultrasonication did not lead to decreases in the protein or lactose content of both raw and pasteurised milk. Kjeldahl nitrogen determinations confirmed that ultrasonication had no detrimental effect on the total protein or casein content of pasteurised milk. This study indicated that ultrasonication lead to an increase in the fat concentration. This was explained by the larger surface area of the fat globules after ultrasonication, which led to an increase in light scattering as observed by the MilkoScan. Alkaline phosphatase and lactoperoxidase activities were also investigated as potential indicators of an effective ultrasonic treatment. Ultrasonication was, however, found to be ineffective in deactivating both enzymes used regularly by the dairy industry as indicators of effective thermal processes. Résumé -Impact des ultrasons sur les microbes d'altération du lait et sur ses composants.De nombreuses publications suggèrent que dans l'industrie laitière, la mauvaise qualité du produit fini peut provenir de défaillances de la pasteurisation. Le but de cette étude a été d'évaluer l'utilisation de l'ultrasonication comme alternative au traitement thermique. Les résultats ont montré que les ultrasons éliminaient totalement, ou à des niveaux acceptables par la législation sud-africaine et anglaise, la flore d'altération et les pathogènes potentiels, et ce même quand les charges d'inoculum initiales avant traitement étaient 5 fois supérieures à celles autorisées. La réduction du nombre de cellules viables après ultrasonication était de 100 % après 10.0 min pour E. coli, de 100 % après 6.0 min pour Pseudomonas fluorescens et de 99 % après 10.0 min pour Listeria monocytogenes. L'analyse par infrarouge du lait cru et du lait pasteurisé après traitement aux ultrasons ne montrait aucune diminution de la teneur en protéines et en lactose dans ces deux laits. La quantification de l'azote par Kjeldahl confirmait l'absence d'effet préjudiciable de l'ultrasonication sur les teneurs en protéines totales ou en caséines du lait pasteurisé. Cette étude montre que l'ultrasonication conduit à ...

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“…Additionally, it was verified that the impact of the nutrient load became slightly more important in dynamic conditions. Dewanti and Wong (1995) studied the effect of culture conditions on biofilm formation by an E. coli strain isolated from ground beef. These authors verified that initial cell adhesion and biofilm formation in dynamic conditions were higher and faster in low nutrient media.…”

Section: Resultsmentioning

confidence: 99%

The impact of material properties, nutrient load and shear stress on biofouling in food industries

Moreira

Gomes

Simões

et al. 2015

Food and Bioproducts Processing

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Abstract:In the food industry, biofilm formation in pipes, equipment and cooling systems increases maintenance costs, decreases operational efficiencies and is a source of contamination.Shear stress, nutrient load and surface material are important variables affecting the biofilm onset in industry. In this work, the combined impacts of these variables were assessed using three different materials (glass, copper and stainless steel), two nutrient loads (high and low nutrient medium) and two hydrodynamic conditions (static and dynamic). Initial adhesion and biofilm formation were studied in microplates usingEscherichia coli as a model organism.Surface material was the factor with the strongest impact and adhesion/biofilm formation were correlated with surface hydrophobicity. However, the impact of this variable was dependent on the nutrient load and imposed shear stress. It was also found that, for the majority of the situations tested, initial attachment performance is a good predictor of biofilm formation behaviour and that the effects observed during attachment are amplified during biofilm maturation. Since shear stress is a major determinant in cell adhesion, the results of this study may find application in industrial systems operating at flow rates between 0.001 and 600 m 3 h -1 depending on tube material and diameter.

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Influence of culture conditions on biofilm formation by Escherichia coli O157:H7

Cited by 238 publications

References 36 publications

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Impact of ultrasound on dairy spoilage microbes and milk components

The impact of material properties, nutrient load and shear stress on biofouling in food industries

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