Influence of culture conditions on the production of extracellular polymeric substances (EPS) by Arthrospira platensis

Silva, Mariana Barbalho; Azero, Edwin G.; Teixeira, Cláudia Maria Luz Lapa; Andrade, Cristina T.

doi:10.1186/s40643-020-00337-3

Cited by 17 publications

(10 citation statements)

References 51 publications

(67 reference statements)

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“…The data also show high variations between the ratios of the individual components and a clear dependency between the obtained product and the extraction technique used, which makes a standardized production process necessary. These findings are in accordance with several other studies, confirming that changes in culture conditions can influence the release and molecular composition of microalgae metabolites [ 89 , 90 , 91 ]. It is a common practice to induce the biosynthesis of target metabolites, such as pigments, EPS and lipids by changes in nutrient, salinity and light availability [ 22 , 69 , 74 , 92 , 93 , 94 ].…”

Section: Discussionsupporting

confidence: 93%

Potential of the Red Alga Dixoniella grisea for the Production of Additives for Lubricants

Gavalás-Olea

Siol

Sakka

et al. 2021

Plants

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There is an increasing interest in algae-based raw materials for medical, cosmetic or nutraceutical applications. Additionally, the high diversity of physicochemical properties of the different algal metabolites proposes these substances from microalgae as possible additives in the chemical industry. Among the wide range of natural products from red microalgae, research has mainly focused on extracellular polymers for additive use, while this study also considers the cellular components. The aim of the present study is to analytically characterize the extra- and intracellular molecular composition from the red microalga Dixoniella grisea and to evaluate its potential for being used in the tribological industry. D. grisea samples, fractionated into extracellular polymers (EPS), cells and medium, were examined for their molecular composition. This alga produces a highly viscous polymer, mainly composed of polysaccharides and proteins, being secreted into the culture medium. The EPS and biomass significantly differed in their molecular composition, indicating that they might be used for different bio-additive products. We also show that polysaccharides and proteins were the major chemical compounds in EPS, whereas the content of lipids depended on the separation protocol and the resulting product. Still, they did not represent a major group and were thus classified as a potential valuable side-product. Lyophilized algal fractions obtained from D. grisea were found to be not toxic when EPS were not included. Upon implementation of EPS as a commercial product, further assessment on the environmental toxicity to enchytraeids and other soil organisms is required. Our results provide a possible direction for developing a process to gain an environmentally friendly bio-additive for application in the tribological industry based on a biorefinery approach.

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Section: Discussionsupporting

confidence: 93%

Potential of the Red Alga Dixoniella grisea for the Production of Additives for Lubricants

Gavalás-Olea

Siol

Sakka

et al. 2021

Plants

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“…Li et al [ 44 ] found that P. purpureum exhibited the highest EPS content when cultured at low initial nitrogen concentrations (3.5 mM). Silva et al [ 45 ] reported that the best EPS yield in A. platensis was achieved under the lowest NaNO 3 concentration (0.25 g L −1 ). Additionally, Cepák and Přibyl [ 46 ] demonstrated that the most abundant EPS levels in B. braunii were produced at a moderate nitrogen concentration of 6 mM.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Exopolysaccharides Production by Porphyridium sordidum and Their Potential to Induce Defense Responses in Arabidopsis thaliana against Fusarium oxysporum

et al. 2021

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Polysaccharides from marine algae are one novel source of plant defense elicitors for alternative and eco-friendly plant protection against phytopathogens. The effect of exopolysaccharides (EPS) produced by Porphyridium sordidum on elicitation of Arabidopsis thaliana defense responses against Fusarium oxysporum was evaluated. Firstly, in order to enhance EPS production, a Box–Behnken experimental design was carried out to optimize NaCl, NaNO3 and MgSO4 concentrations in the culture medium of microalgae. A maximum EPS production (2.45 g/L) higher than that of the control (0.7 g/L) was observed for 41.62 g/L NaCl, 0.63 g/L NaNO3 and 7.2 g/L MgSO4 concentrations. Structurally, the EPS contained mainly galactose, xylose and glucose. Secondly, the elicitor effect of EPS was evaluated by investigating the plant defense-related signaling pathways that include activation of Salicylic or Jasmonic Acid-dependent pathway genes. A solution of 2 mg/mL of EPS has led to the control of fungal growth by the plant. Results showed that EPS foliar application induced phenylalaline ammonia lyase and H2O2 accumulation. Expression profile analysis of the defense-related genes using qRT-PCR revealed the up-regulation of Superoxide dismutases (SOD), Peroxidase (POD), Pathogenesis-related protein 1 (PR-1) and Cytochrome P450 monooxyge-nase (CYP), while Catalase (CAT) and Plant defensin 1.2 (PDF1.2) were not induced. Results suggest that EPS may induce the elicitation of A. thaliana’s defense response against F. oxysporum, activating the Salicylic Acid pathway.

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“…Generally, there is a positive effect of high light intensity on EPS (Delattre et al, 2016). When comparing different reports, one could conclude that EPS production is enhanced at low irradiations by increasing the light supply, leading to a light inhibition at high light intensities (Trabelsi et al, 2009;Delattre et al, 2016;Silva et al, 2020). The optimum light supply strongly depends on the production organism (Trabelsi et al, 2009;Silva et al, 2020) and the reactor system (Iqbal and Zafar, 1993).…”

Section: Introductionmentioning

confidence: 99%

Influence of Process Operation on the Production of Exopolysaccharides in Arthrospira platensis and Chlamydomonas asymmetrica

Jung¹,

Zell²,

Boßle³

et al. 2022

Front. Sustain. Food Syst.

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Extracellular polysaccharides, or exopolysaccharides are high–molecular weight sugar-based polymers expressed and secreted by many microorganisms. As host organisms, the functions of exopolysaccharides are diverse, ranging from physical protection via biofilm formation, adhesion, and water retention to biological functions that are not entirely understood such as viral attachment inhibition. Industrial applications of exopolysaccharides can be found in food texture modification; for example, utilizing the hydrocolloidal properties of exopolysaccharides for thickening and gelling purposes to improve food quality and texture. Over the last decade, biologically active exopolysaccharides produced by microalgae have received attention for their potential as antiviral, antibacterial and antioxidative compounds and in the applications. However, relatively low yield and productivity are the limiting factors for full-scale industrial application. In this study, the well-known prokaryotic phototrophic microorganism Arthrospira platensis and the comparatively unknown eukaryotic unicellular green alga Chlamydomonas asymmetrica were used to evaluate the influence of different process parameters on exopolysaccharides formation and productivity. In addition to the essential control variables (light and temperature), the influence of operational techniques (batch and turbidostat) were also investigated. Although the two studied algae are differently affected by above parameters. The light intensity was the most influential parameter observed in the study, leading to differences in exopolysaccharides concentrations by a factor of 10, with the highest measured concentration for A. platensis of cEPS = 0.138 g L−1 at 180 μmol m−2 s−1 and for C. asymmetrica of cEPS = 1.2 g L−1 at 1,429 μmol m−2 s−1. In continuous systems, the achieved exopolysaccharides concentrations were low compared to batch process, however, slightly higher productivities were reached. Regardless of all screened process parameters, C. asymmetrica is the better organism in terms of exopolysaccharides concentrations and productivity.

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Influence of culture conditions on the production of extracellular polymeric substances (EPS) by Arthrospira platensis

Cited by 17 publications

References 51 publications

Potential of the Red Alga Dixoniella grisea for the Production of Additives for Lubricants

Potential of the Red Alga Dixoniella grisea for the Production of Additives for Lubricants

Optimization of Exopolysaccharides Production by Porphyridium sordidum and Their Potential to Induce Defense Responses in Arabidopsis thaliana against Fusarium oxysporum

Influence of Process Operation on the Production of Exopolysaccharides in Arthrospira platensis and Chlamydomonas asymmetrica

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