Influence of culture conditions on extracellular polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Nostoc flagelliforme

Han, Peipei; Yao, Shunyu; Guo, Rong-jun; Yan, Rong-rong; Wu, Yi-Kai; Shen, Shi-gang; Jia, Shiru

doi:10.1039/c7ra07982f

Cited by 16 publications

(10 citation statements)

References 58 publications

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“…Interestingly, among the four different sources of nitrogen, urea promoted the highest amount of RPS in Nostoc flagelliforme [61]. The absence of inorganic nitrogen, i.e., diazotrophic conditions, was reported to influence positively the production of EPS in certain heterocystous forming [62][63][64] and unicellular species [65].…”

Section: Culture Mediummentioning

confidence: 99%

“…Much work has been done on Nostoc flagelliforme [11,61,63,[79][80][81][82][83][84]. This strain is an edible terrestrial cyanobacterium that is used as food in China for more than 2000 years.…”

Section: Process Conditionsmentioning

confidence: 99%

“…Optimization of carbon, nitrogen source, and light quality reinforced the fact that growth and EPS have their own requirements. In addition, these culture conditions also affected the EPS-associated enzymes, showing a correlation with the number of EPS produced [63]. More recently, a transcriptomic analysis revealed that light quality regulated EPS biosynthesis via the intracellular reactive oxygen species (ROS) level directly other than oxidative stress of N. flagelliforme [83].…”

Section: Process Conditionsmentioning

confidence: 99%

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Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

et al. 2020

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Cyanobacteria have the potential to become an industrially sustainable source of functional biopolymers. Their exopolysaccharides (EPS) harbor chemical complexity, which predicts bioactive potential. Although some are reported to excrete conspicuous amounts of polysaccharides, others are still to be discovered. The production of this strain-specific trait can promote carbon neutrality while its intrinsic location can potentially reduce downstream processing costs. To develop an EPS cyanobacterial bioprocess (Cyano-EPS) three steps were explored: the selection of the cyanobacterial host; optimization of production parameters; downstream processing. Studying the production parameters allow us to understand and optimize their response in terms of growth and EPS production though many times it was found divergent. Although the extraction of EPS can be achieved with a certain degree of simplicity, the purification and isolation steps demand experience. In this review, we gathered relevant research on EPS with a focus on bioprocess development. Challenges and strategies to overcome possible drawbacks are highlighted.

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Section: Culture Mediummentioning

confidence: 99%

Section: Process Conditionsmentioning

confidence: 99%

Section: Process Conditionsmentioning

confidence: 99%

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Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

et al. 2020

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“…However, in terms of light quality, it has been shown that different wavelengths can enhance the sustainability of cyanobacteria cultures according to the target of production (Pagels et al, 2019). For instance, multiple studies reported that red light (650 nm) and blue light could promote the production of EPS or accelerate metabolic paths in cyanobacteria from genera Nostoc, Synechocystis and Synechococcus (Bland & Angenent, 2016;Han et al, 2017a;Han et al, 2017b;Ooms et al, 2017), and the microalgae Chlorella (Atta et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Ortiz-Moreno¹,

Cárdenas-Poblador²,

Agredo³

et al. 2020

Univ. Sci.

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Mathematical models provide information about population dynamics under different conditions. In the study, four models were evaluated and employed to describe the growth kinetics of Nostoc ellipsosporum with different light wavelengths: Baranyi-Roberts, Modiﬁed Gompertz, Modiﬁed Logistic, and Richards. N. ellipsosporum was grown in BG-11 liquid medium for 9 days, using 12 hours of photoperiod and the following treatments: white light (400-800 nm), red light (650-800 nm), yellow light (550-580 nm) and blue light (460-480 nm). Each experiment was performed in triplicate. The optical density (OD) was measured on days 1, 3, 5, 7 and 9, using a spectrophotometer at 650 nm. The maximum cell growth was obtained under white light (OD650 : 0.090 ± 0.008), followed by the yellow light (OD650 :0.057 ± 0.004). Conversely, blue light showed a marked inhibitory effect on the growth of N. ellipsosporum (OD650 : 0.009 ± 0.001). The results revealed that the Baranyi-Roberts model had a better ﬁt with the experimental data from N. ellipsosporum growth in all four treatments. The ﬁndings from this modeling study could be used in several biotechnological applications that require the productionof N. ellipsosporum and its bioproducts.

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“…In addition, the results of transcriptome showed that the expressions of four genes involved in polysaccharide synthesis were increased compared with the control (Table 2). Researches demonstrated that increasing gene expression of UDP-glucose pyrophosphorylase (UGP), α-phosphoglucomutase (PGM), phosphoglucose isomerase (PGI), and phosphomannose isomerase (PMI) facilitated polysaccharide production (Yang et al, 2013; Li et al, 2015; Xu et al, 2015; Han et al, 2017). Therefore, L-phenylalanine treatment increased the expression level of four genes related to polysaccharide synthesis, which should be one of the reasons for the increase of polysaccharide production.…”

Section: Resultsmentioning

confidence: 99%

Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer

Wang

et al. 2019

Front. Microbiol.

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Ganoderma lucidum has been a well-known species of basidiomycetes for a long time, and has been widely applied in the fields of food and medicine. Based on the simulation results of model iZBM1060 in our previous research, the effect of L-phenylalanine on G. lucidum extracellular polysaccharides (EPSs) was investigated in this study. EPS production reached 0.91 g/L at 0.4 g/L L-phenylalanine after a 24 h culture, which was 62.5% higher than that of control (0.56 g/L). Transcriptome and genome analysis showed that L-phenylalanine deaminase and benzoate 4-hydroxylase (related to L-phenylalanine metabolism) were significantly up-regulated, while the cell wall mannoprotein gene was down-regulated. Transmission electronic microscopy (TEM) and atomic force microscopy results showed that the cell wall thickness decreased by 58.58%, and cell wall porosity increased in cells treated with L-phenylalanine, which probably contribute to the increasing EPS production. This study provides an efficient strategy for fungal polysaccharide production with high output and low cost.

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Influence of culture conditions on extracellular polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Nostoc flagelliforme

Abstract: Important enzymes influencing the production of Nostoc flagelliforme EPS were investigated under different culture conditions.

Cited by 16 publications

References 58 publications

Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

Modeling the effects of light wavelength on the growth of Nostoc ellipsosporum

Development of an Efficient Strategy to Improve Extracellular Polysaccharide Production of Ganoderma lucidum Using L-Phenylalanine as an Enhancer

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