Influence of cultivation factors on morphogenesis in vitro Salix acutifolia Willd

Abstract

Salix contains salicylates in their leaves, bark, and buds. That is why there is a necessity in elaborating microcloning propagation technologies of same genotypes which are high productive in salicin in order to create industrial plantations. The study examined the effect of 25 different combinations of BAP and NAA concentrations on the growth and development of S. acutifolia. The best results were observed on MS supplemented with 1.0 mg/l BAP and 0.01 or 0.1 mg/l NAA, and rhizogenesis on MS medium supplemented with 0.1 and 0.2 mg/l NAA. In order to study the effect of the mineral composition of basic media on the morphogenesis of S. acutifolia in an in vitro culture, DKW, GD, MS, and WPM were tested. Kn, BAP, and 2iP were compared to study the effect of cytokinins on the morphogenetic ability of S. acutifolia explants. The influence of the mineral composition of the medium on the rhizogenesis of S. acutifolia explants in vitro was evaluated on four studied media DKW, GD, WPM, and MS. Each medium contained 3% sucrose, 0.6% agar-agar, and 0.2 mg/l NAA. As a control, MS medium supplemented with 0.2 mg/l NAA was selected.

Protocol for In Vitro Propagation of Salix acmophylla (Boiss.). Studies on Three Ecotypes

“…Amo-Marco and Lledo [21], after a test using three auxins (IBA, IAA and NAA) at different concentrations, recorded a high rooting percentage (90-100%) on all the media tested, including control, but observing a negative effect with NAA, with the outgrowth of abnormally thick roots and excessive callus production at the base of the shoots. In S. acutifolia Willd., however, the best rooting performances were obtained after 8 weeks on media containing NAA 0.2 mg L −1 , reaching 97 and 93% rooting on MS and WPM, respectively [57].…”