Influence of cryopreservation on human periodontal ligament cells in vitro

Temmerman, Liesbeth; Dermaut, Luc; Mil, Martine De; Maele, Georges Van; Beele, Hilde; Pauw, Guy A.M. De

doi:10.1007/s10561-007-9047-y

Cited by 22 publications

(19 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One of the reasons can be that there are still a lot of questions about the different reactions in the dental tissue following cryopreservation. Although several reports about this topic have been published (Bartlett and Reade 1972;Price and Cserepfalvi 1972;Schwartz and Andreasen 1983;Schwartz et al 1985;Schwartz and Rank 1986;Schwartz 1986;Politis et al 1995;Laureys et al 2001;Kawasaki et al 2004;Oh et al 2005;Izumi et al 2007;Temmerman et al 2008), these papers concentrate mainly on the periodontal tissues, because it is generally known that a healthy periodontal ligament (PDL) is an important factor to evaluate success after transplantation. Moreover, a good healing of the PDL is necessary to move the transplanted tooth orthodontically.…”

Section: Introductionmentioning

confidence: 99%

Influence of cryopreservation on the pulpal tissue of immature third molars in vitro

et al. 2009

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to evaluate in vitro the viability of isolated and non-isolated pulpal tissue of immature third molars after cryopreservation. This study was divided in three different experiments. Experiment 1: Pulpal tissue isolated from 19 third molars was divided in horizontal segments. Each segment was cultured separately in order to evaluate whether differences in growth capacity within the tissue could be found. Experiment 2: Pulpal tissue isolated from 27 third molars was divided in a mesial and a distal part. One part was cryopreserved before culturing, the other part was cultured immediately. Growth capacity of cryopreserved and non-cryopreserved tissue was evaluated and compared. Experiment 3: 43 third molars were cryopreserved. After thawing, the dimension of the apical foramen was measured and the pulp was isolated and segmented horizontally. The different parts were cultured and growth capacity was evaluated and compared. Results of experiment 1 and 2 showed no significant difference in growth capacity between fibroblasts originating from different pulpal segments of the same tooth without cryopreservation and between fibroblasts originating from cryopreserved and non-cryopreserved isolated pulpal tissue. In experiment 3 it was demonstrated that the dimension of the apical foramen and pulpal viability after cryopreservation are positively correlated. A minimum dimension of 9.42 mm(2) enables the cryoprotective agent to penetrate sufficiently and to protect the pulpal tissue from apex to crown. This study proved that cryopreservation of human pulpal tissue is possible if the cryoprotective agent can reach the entire pulp.

show abstract

Section: Introductionmentioning

confidence: 99%

Influence of cryopreservation on the pulpal tissue of immature third molars in vitro

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…As células mesenquimais de origem dental têm sido submetidas à criopreservação e sua atividade biológica vem sendo testada após descongelamento por diversos autores 15,17,19 . Esses estudos mostram a manutenção tanto da viabilidade e do potencial proliferativo das células, como ficou evidente no presente trabalho, quanto da sua potencialidade de diferenciação em diversos tipos celulares.…”

Section: Discussionunclassified

“…Portanto, o processo de congelamento ideal deve evitar a formação de cristais de gelo no interior das células e posterior lesão celular. Para isso, deve-se aplicar uma velocidade de congelamento adequada, de acordo com o tipo celular em questão 17 .…”

Section: Introductionunclassified

“…A maioria dos estudos visa a determinar se o processo de criopreservação afeta a capacidade proliferativa ou o potencial de diferenciação das células criopreservadas em comparação com células não submetidas à criopreservação 15,17,18 Para a obtenção das células do ligamento periodontal, foram utilizados dois dentes permanentes humanos hígidos (terceiros molares), removidos por indicação ortodôntica. Após a exodontia, os elementos dentários foram imediatamente armazenados em tubos tipo Falcon de 50 mL (TTP  , USA) contendo 10 mL de meio de cultura alfa-MEM (Cultilab, Brasil).…”

Section: Introductionunclassified

See 1 more Smart Citation

Rendimento de células mesenquimais do ligamento periodontal humano submetidas a diferentes protocolos de criopreservação

Soares¹,

Ginani²,

Barboza³

2012

Rev. odontol. UNESP

View full text Add to dashboard Cite

INTRODUÇÃO: A técnica de criopreservação tem como característica cessar reversivelmente todas as funções biológicas dos tecidos vivos em baixas temperaturas e tem sido aplicada a diversas células humanas, visando à sua utilização posterior. OBJETIVO: Avaliar a proliferação de células mesenquimais do ligamento periodontal humano após a criopreservação por dois diferentes protocolos. MÉTODO: As células do ligamento periodontal foram obtidas a partir de dois dentes (terceiros molares) hígidos, com indicação de remoção cirúrgica. Após o processamento, as células foram cultivadas em placas de Petri e mantidas a 37 °C em 5% de CO2, até atingirem 70-90% de confluência, com troca de meio a cada três dias. Na primeira passagem, as células foram divididas em dois grupos e criopreservadas: Grupo -80 °C - criopreservação em ultrafreezer por 45 dias; Grupo -196 °C - criopreservação em nitrogênio líquido por 45 dias. Decorrido esse tempo, as células dos dois grupos foram descongeladas e plaqueadas para o experimento. A curva de crescimento dos grupos estudados foi traçada a partir de contagem em Câmara de Neubauer e pelo método de ensaio do MTT, nos intervalos de 24, 48 e 72 horas. Os resultados foram analisados por meio do teste de Mann‑Whitney, com nível de significância de 5%. RESULTADO: Verificou-se um crescimento ascendente nos dois protocolos utilizados, porém uma maior taxa proliferativa foi verificada no grupo criopreservado em nitrogênio líquido (p < 0,05). CONCLUSÃO: Ambos os protocolos de criopreservação estudados foram eficazes, porém a criopreservação em nitrogênio líquido (-196 °C) manteve uma maior taxa de proliferação celular em todos os intervalos de tempo.

show abstract

“…Successful autotransplantation and allotransplantation of cryopreserved teeth using this technique have been reported [2,3]. The conventional cryopreservation method has been proven to maintain the membrane integrity, viability, and differentiation capability of PDL cells [3-5]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of vitrification for cryopreservation of teeth

Dissanayake

Zhong

Choi

et al. 2010

J Periodontal Implant Sci

View full text Add to dashboard Cite

PurposeThe aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking.MethodsIn step 1, primary cultured human PDL cells were cryopreserved in 100% conventional cryopreservation media and 100% vitrification media (ESF40 media) in different temperatures for 2 weeks. In step 2, a series of modified vitrification formulae named T1 (75% vitrification media + 25% F-media), T2 (50% vitrification media + 50% F-media) and T3 (25% vitrification media + 75% F-media) were used to store PDL cells for 2 weeks and 4 weeks in liquid nitrogen. MTT assay was performed to examine the viability of PDL cells.ResultsMaximum cell viability was achieved in cells stored in 100% conventional cryopreservation media at -196℃ (positive control group) in step 1. Compared to the positive control group, viability of the cells stored in 100% vitrification media was very low as 10% in all test conditions. In step 2, as the percentage of vitrification media decreased, the cell viability increased in cells stored for 2 weeks. In 4-week storage of cells in step 2, higher cell viability was observed in the T2 group than the other vitrification formulae while the positive control group had the highest viability. There was no statistically significant difference in the cell viability of 2-week and 4-week stored cells in the T2 group.ConclusionsThese observations indicate 100% vitrification media is not successful in PDL cell cryopreservation. Conventional cryopreservation media is currently the most appropriate media type for this purpose while T2 media would be interesting to test for long-term storage of PDL cells.

show abstract

Influence of cryopreservation on human periodontal ligament cells in vitro

Cited by 22 publications

References 29 publications

Influence of cryopreservation on the pulpal tissue of immature third molars in vitro

Influence of cryopreservation on the pulpal tissue of immature third molars in vitro

Rendimento de células mesenquimais do ligamento periodontal humano submetidas a diferentes protocolos de criopreservação

Evaluation of vitrification for cryopreservation of teeth

Contact Info

Product

Resources

About