Influence of corpus luteum and ovarian volume on the number and quality of bovine oocytes

Penitente-Filho, Jurandy Mauro; Jimenez, Carolina Rodrigues; Zolini, Adriana Moreira; Carrascal, Erly; Azevedo, Jovana Luiza de; Silveira, Camila; Oliveira, Fabrício Marques de; Torres, Ciro Alexandre Alves

doi:10.1111/asj.12261

Cited by 23 publications

(12 citation statements)

References 23 publications

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“…Furthermore, the ovarian follicular reserve can also be evaluated through ovarian volume (OV) (Kwee et al, 2007), which varies in accordance with the reproductive stage, the oestrous phase and the gestation period, and can be used as a parameter in the clinical and functional evaluation of the organ (Chacur et al, 2006). Ovarian volume can be used as a reproductive parameter because it has a direct relationship with hormone production during the oestrous cycle (Penitente-Filho et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes

Vasconcelos

Maculan

Cunha

et al. 2020

Zygote

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Summary This study aimed to evaluate the relationship between antral follicular count (AFC) and ovarian volume (OV), preantral follicular population and survival, meiotic progression and ultrastructure of cumulus–oocyte complexes (COCs) after in vitro maturation. In experiment 1, the relationship between AFC and preantral follicle population and survival was evaluated by classical histology. In experiment 2, the relationship among AFC, OV, ability of oocytes to resume meiosis and ultrastructure of in vitro matured bovine COCs was studied. A positive correlation (P < 0.05) between AFC and the numbers of healthy primordial, degenerate and total follicles was observed, as well as with healthy secondary follicles and total follicles. The numbers of grades I and II oocytes in ovaries of high AFC class were higher compared with those with intermediate or lower AFC. After in vitro maturation, COCs from ovaries of high AFC had a higher percentage of oocytes in metaphase II compared with those of intermediate and low AFC (P < 0.0001). Ovaries of intermediate AFC had a higher percentage of oocytes in metaphase II compared with ovaries with low AFC (P < 0.0001). The proportion of oocytes in metaphase I, telophase I and anaphase I in COCs from ovaries of intermediate AFC (26.04%) was higher (P < 0.05) compared with that seen in COCs of ovaries with high (8.55%) and low (14.15%) AFC. No differences in the ultrastructure of oocytes were seen. In conclusion, after in vitro maturation, cow ovaries with high AFC have higher numbers of oocytes that reach in metaphase II (MII), but they also have higher numbers of degenerated primordial and primary follicles.

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Section: Introductionmentioning

confidence: 99%

Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes

Vasconcelos

Maculan

Cunha

et al. 2020

Zygote

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“…In agreement with this notion, Pirestani et al [19] reported that oocytes derived from ovaries containing a large follicle (~20 mm) were less competent compared to embryos derived from ovaries that containing a CL. Similarly, Penitente-Filho et al [20] classified cumulus-oocyte complexes (COCs) under the stereomicroscope and indicated that ovaries with CL yielded a greater number of good quality oocytes than ovaries without CL. However, the oocytes used in the latter study were not subjected to IVP to confirm their developmental competence.…”

Section: Ovarian Morphologymentioning

confidence: 99%

Oocyte Selection for in Vitro Embryo Production in Livestock Species: Non-Invasive Approaches for the New Challenges of Oocyte Competence

Águila¹,

Treulén²,

Therrien³

et al. 2020

Preprint

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The efficiency of producing embryos using in vitro technologies in livestock species rarely exceeds the 30 to 40% threshold, indicating that the proportion of oocytes that fail to develop after in vitro fertilization and culture is considerably large. Considering that the intrinsic quality of the oocyte is one of the main factors affecting blastocyst yield, the precise identification of non-invasive cellular or molecular markers that predict oocyte competence is of major interest to research and practical applications. The aim of this review was to explore the current literature on different non-invasive markers associated with oocyte quality in mammalian species, with an emphasis on the bovine model. Apart from some controversial findings, the presence of cycle-related structures in ovaries, a follicle size between 6 and 10 mm, large number of surrounding cumulus cells, slightly expanded investment without dark areas, large oocyte diameter (&gt;120 microns), dark cytoplasm, and the presence of a round and smooth first polar body have been associated to better competence. In addition, the combination of oocyte and zygote selection by BCB test and spindle imaging have the potential to further optimize the identification of oocytes with better developmental competence for in vitro-derived technologies in livestock species.

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“…Hambatan sekresi estrogen dan inhibin oleh progesteron akan menekan pengaruh negatif folikel dominan terhadap pertumbuhan dan perkembangan folikel subordinat lainnya (Parera dan Lenda, 2019). Selain itu hormon progesteron memungkinkan folikel terekspos untuk periode yang lebih lama sebagai akibat dari rendahnya level LH dan hal ini memungkinkan untuk memperoleh oosit yang berkualitas baik (Penitente-Filho et al, 2015). Sebaliknya pada ovarium tanpa CL, tidak adanya kehadiran CL tidak menyebabkan efek negatif progesteron pada hipofisis anterior sehingga kadar estrogen dan progesteron tetap seimbang yang memungkinkan pertumbuhan folikel (Khandoker et al, 2016).…”

Section: Hasil Dan Pembahasan Pengaruh Status Reproduksi Terhadap Kuaunclassified

Pengaruh Keberadaan Corpus Luteum Terhadap Kualitas Oosit dan Tingkat Maturasi Oosit Kerbau Secara In Vitro

Raharjo¹,

Udin

Hendri

2020

JPI

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Penelitian ini bertujuan untuk mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium kerbau terhadap kualitas oosit dan tingkat maturasi oosit secara in vitro. Sebanyak 30 ovarium yang berasal dari RPH kemudian dikelompokkan berdasarkan keberadaan CL. Setelah itu oosit dikoleksi secara slicing, oosit yang diperoleh dikelompokkan berdasarkan kekompakan sel kumulus dan status sitoplasma. Maturasi pada medium Tissue Culture Medium-199 (TCM-199) yang ditambahkan Penstrep, Pregnant Mare Serum Gonadotrophin (PMSG), Hormone Chorionic Gonadotrophin (hCG) dan 3% BSA. Maturasi in vitro dilakukan pada suhu 38,5 o C selama 24 jam di dalam inkubator dengan konsentrasi CO 2 5%. Evaluasi tingkat kematangan inti diklasifikasikan menjadi germinal vesicle (GV), germinal vesicle break down (GVBD), metafase I (MI), Anafase/telophase (A/T) dan metafase II (MII). Hasil penelitian menujukkan status reproduksi tidak signifikan (P>0,05) mempengaruhi kualias oosit kerbau. Persentase oosit berkualitas baik (Grade A dan B) pada masing-masing kelompok adalah 53,76% dan 50,19%. Selanjutnya hasil analisis statistik menunjukkan tidak terdapat perbedaan nyata (P>0,05) pada tingkat maturasi di antara kedua kelompok ovarium. Persentase oosit yang mencapai tahap MII adalah sebesar 61,22% dan 62,62% pada masing-masing kelompok. Dari penelitian ini dapat disimpulkan kualitas oosit dan tingkat maturasi oosit yang mampu mencapai tahap MII tidak signifikan dipengaruhi oleh status reproduksi ovarium.

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Influence of corpus luteum and ovarian volume on the number and quality of bovine oocytes

Cited by 23 publications

References 23 publications

Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes

Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes

Oocyte Selection for in Vitro Embryo Production in Livestock Species: Non-Invasive Approaches for the New Challenges of Oocyte Competence

Pengaruh Keberadaan Corpus Luteum Terhadap Kualitas Oosit dan Tingkat Maturasi Oosit Kerbau Secara In Vitro

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