Influence of Consistency and Composition of Growth Medium on Surface Physicochemical Properties of Streptomyces

Othmany, Rabha El; Zahir, Hafida; Zanane, Chorouk; Ellouali, Mostafa; Latrache, Hassan

doi:10.22207/jpam.15.3.67

Cited by 2 publications

(3 citation statements)

References 37 publications

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“…The interaction of CTAB with the bacterial membrane affects its viability, as do the pH and osmolarity of the medium, starvation, extreme temperatures, oxygen concentration, amount of glucose, bactericidal agents or the bacterial metabolic state itself [52][53][54][55]. In particular, in the study by Sathya et al [56], the effects of CTAB nanocomposites on the bacterial surface structure were investigated using strains with different surface properties.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Response to the Surface Aging of PLA Matrices Loaded with Active Compounds

et al. 2022

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The use of active components in biomaterials improves the properties of existing ones and makes it possible to obtain new devices with antibacterial properties that prevent infections after implantation, thus guaranteeing the success of the implant. In this work, cetyltrimethylammonium bromide (CTAB) and magnesium particles were incorporated into polylactic acid (PLA) films to assess the extent to which progressive aging of the new surfaces resists bacterial colonization processes. For this purpose, the films’ surface was characterized by contact angle measurements, ToF-SIMS and AFM, and adhesion, viability and biofilm growth of Staphylococcus epidermidis bacteria on these films were also evaluated. The results show that the inclusion of Mg and CTAB in PLA films changes their surface properties both before and after aging and also modifies bacterial adhesion on the polymer. Complete bactericidal activity is exhibited on non-degraded films and films with CTAB. This antibacterial behavior is maintained after degradation for three months in the case of films containing a higher amount of CTAB.

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Section: Discussionmentioning

confidence: 99%

Bacterial Response to the Surface Aging of PLA Matrices Loaded with Active Compounds

et al. 2022

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“…Othmany et al [ 19 ] studied the electron donor and acceptor character of six Streptomyces strains using the contact angle method. They reported that these strains have a stronger electron donor character and a weak acceptor character.…”

Section: Discussionmentioning

confidence: 99%

“…Two methods the contact angle method and the microbial hydrocarbon adhesion method (MATH) were used to determine the hydrophobicity of the bacterial surface of Streptomyces. Compared to MATH, the contact angle method, which consists in estimating the surface energy by measuring the contact angle of the drops deposited on the studied surfaces, requires very elaborate, specifc equipment [18][19][20]. In the case of Streptomyces, the microbial hydrocarbon adhesion method (MATH) was frst used by and more recently by Ding and Lammler [21] and N. Alonso [22] to evaluate the surface hydrophobicity.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Streptomyces Cell Surface by the Microbial Adhesion to Solvents Method

Zanane

Taoufik

2023

International Journal of Microbiology

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The cell surface physicochemical properties of Streptomyces should influencing the dispersal and adsorption of spores and hyphae in soil and should conditioning there interactions with organic or metal substances in the bioremediation of contaminated environment. These properties are concerning surface hydrophobicity, electron donor/acceptor, and charge surface. To date, only hydrophobicity of Streptomyces was studied by contact angle measurements and microbial adhesion to hydrocarbons (MATH). In this work, we studied the electron donor/acceptor character of the Streptomyces cell surface in two ionic strength 10−3 M and 10−1 M of KNO3. Thus, to facilitate the characterisation of the surfaces of microbial cells, we used a simple, rapid, and quantitative technique, the microbial adhesion method to solvents (MATS), which is based on the comparison of the affinity of microbial cells for a monopolar solvent with a polar solvent. The monopolar solvent can be acid (electron acceptor) or basic (electron donor), but both solvents should have a surface tension similar to that of the Kifshitz van der Waals components. At the significant ionic strength of the biological medium, the electron donor character is well expressed for all 14 Streptomyces strains with very significant differences among them ranging from 0% to 72.92%. When the cells were placed in a solution with a higher ionic strength, we were able to classify the donor character results into three categories. The first category is that the weak donor character of strains A53 and A58 became more expressed at 10−1 M KNO3 concentration. The second category is that three strains A30, A60, and A63 expressed a weaker character in a higher ionic strength. For the other strains, no expression of the donor trait was obtained at higher ionic strength. In a suspension with a concentration of 10−3 KNO3, only two strains expressed an electron acceptor character. This character is very important for strains A49, A57, A58, A60, A63, and A65 at 10−1M KNO3. This work has shown that these properties vary greatly depending on the Streptomyces strain. It is important to consider the change in physicochemical properties of surface cells with ionic strength when using Streptomyces in different bioprocesses.

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Influence of Consistency and Composition of Growth Medium on Surface Physicochemical Properties of Streptomyces

Cited by 2 publications

References 37 publications

Bacterial Response to the Surface Aging of PLA Matrices Loaded with Active Compounds

Bacterial Response to the Surface Aging of PLA Matrices Loaded with Active Compounds

Characterization of Streptomyces Cell Surface by the Microbial Adhesion to Solvents Method

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